Biological membrane and preparation method thereof

Abstract

The invention relates to the technical field of tissue engineering, in particular to a biofilm and a preparation method thereof. This method has simple process, low cost, less damage to the collagen fibers of membrane tissue, and good decellularization effect. The obtained biofilm retains the natural double-layer structure of animal membrane tissue and can guide tissue regeneration. The clinical application of the membrane has created conditions. The embodiment of the present invention provides a method for preparing a biofilm, comprising: step 1) removing the fatty tissue on the surface of the mammalian membrane tissue to obtain the membrane tissue after the preliminary degreasing; step 2) treating the membrane tissue after the preliminary degreasing with alkali solution soaking for decellularization to obtain decellularized membrane tissue; step 3) soaking the decellularized membrane tissue in an organic solvent, and performing degreasing treatment under vibration to obtain degreased membrane tissue; step 4) degreasing The final membrane tissue was flattened, dried, and sterilized to obtain a biofilm.

Description

technical field [0001] The invention relates to the technical field of guided tissue regeneration, in particular to a biofilm and a preparation method thereof. Background technique [0002] Guided tissue regeneration is an ideal technique to promote periodontal new attachment; this technique is to cover the periodontal tissue defect with a guided tissue regeneration film to prevent fast-growing epithelial cells and fibroblasts from growing into the defect area. Thereby providing a certain space for the growth of periodontal ligament cells and bone cells and promoting the regeneration of periodontal tissue. Therefore, an ideal guided tissue regeneration membrane should have a double-layer structure with a dense layer and a loose layer, the dense layer can block the invasion of soft tissues, and the porous structure of the loose layer is more conducive to the attachment and growth of bone cells. [0003] The existing guided tissue regeneration membranes are mainly obtained th...

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Problems solved by technology

[0003] The existing guided tissue regeneration membranes are mainly obtained through two methods. One method is artificial synthesis, which prepares a dense layer and The loose layer, or compounding hyaluronic acid, calcium salt, etc. on foreign degradable materials, is integrated into a two-layer or three-layer structure guided tissue regeneration film through cross-linking or electrospinning technology, which is obtained by artificial synthesis. The guided tissue regeneration membrane needs to introduce foreign degradable materials, and the products of degradable materials are acidic after degradation, which will cause inflammation. Therefore, the biocompatibility and safety of the guided tissue regeneration membrane are poor; making the guided tissue regeneration The mechanical strength of the membrane becomes poor, and it is prone to collapse, thereby affecting the growth of new tissues; another method is to obtain a biofilm containing only extracellular matrix by decellularizing and degreasing the animal membrane tissue. Layer structure, when the biofilm is used as a guiding tissue regeneration film for periodontal tissue defects, no foreign degradable materials are introduced, and the biocompatibility, safety, and mechanical strength are relatively excellent; however, in the prior art, all Pancreatin, EDTA, and polyethylene glycol octylphenyl ether are mainly used for decellularization of the above-mentioned animal membrane tissues. The decellularization effect of these reagents is not obvious and the efficiency is low. Enzymes have greater damage to the collagen fiber structure of the material

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