The invention relates to a
heparin sodium technique, in particular to a
heparin sodium extracting technique utilizing multi-frequency combination and ultrasonic double-
enzyme reinforcement. Firstly, the scraped
small intestinal mucosa is sucked in a conventional enzymolysis vessel, and refined salt with a proportion of 2.3 percent to 2.8 percent by weight is firstly added thereto before heating, and the pH value is adjusted to 9.0 to 9.2; secondly, when the solution obtained from the first step is heated to 40 DEG C to 45 DEG C, trypsase and
lipase are added, and the proportion is maintained between 10 : 0.5 to 10 : 5, the weight proportion of the double
enzyme in the solution in the first step accounts for 0.3 percent to 0.4 percent, the solution is continuously heated to 50 DEG C to 55 DEG C, the enzymolysis under heat preservation is performed for 2 hours to 5 hours, then the solution is continuously heated to 80 DEG C to 85 DEG C, and the solution is filtered after being insulated for 10 minutes to 20 minutes; and thirdly, the filtered solution obtained from the second step is treated through the conventional steps of adsorbing, washing, eluting, precipitating, dehydrating and
drying, so as to obtain the
heparin sodium product. Compared with the prior art, the invention has the advantages that: firstly, the entire
procedure time is substantially reduced by 33 percent; secondly, the yield of products is increased by 29.6 percent; and thirdly, the
titer is stabilized to be higher than 120 (the
titer of the domestic advanced technology product is between 90 and 110).