102 results about "Small intestinal mucosa" patented technology

The invention provides a method for preparing an SIS tissue restoring material. The method comprises the following steps: carrying out sterilizing treatment, degreasing treatment, cell free treatment and absterging treatment according to an arbitrary sequence at the lower layer of small intestinal mucosa; and finally preparing the material through freezing and drying. The SIS tissue restoring material can be used as an independent and common support vector for a growth factor, a cell, a drug and the like, and becomes a tissue engineering material through in-vitro culture. The material is a natural and nontoxic biological material without antigenicity and immunity. The SIS tissue restoring material can be used for restoring hernia, defect of skin, urinary system tissue defect, mucosa defect and other periphery and internal parenchyma defects.

The invention relates to a preparation and using method of medical biocompatible stent material. The medical biocompatible stent material is prepared by applying the small intestine submucosal and the tendon tissue of people or animals as the raw materials. The invention has good biocompatibility, appropriate porosity and mechanical strength and stable spatial structure, and the invention contains various growth factors to induce the tissue for regeneration and reconstruction, thereby improving the success rate of the repair of the various tissue defects and the reconstruction of tissue structures, therefore, the invention is an ideal tissue engineering material. The medical biocompatible stent material which is provided by the invention is applicable to the preparation of various tissue engineering equipments and the application in the repair of various tissue defects and injuries, including skin, blood vessels, nerves, tendons, bones, cartilages, abdominal walls, urinary tracts, oral mucosa and so on, and the invention can be used in the treatment of trauma, abdominal external hernia, anal fistula, rectovaginal fistula, rectum anterior protrusion, bone and cartilage defects, vascular injuries, nerve injuries, tendon injuries, oral mucosal injuries, relaxed pelvic floor, urinary tract injuries and other diseases.

Compositions suitable for use as adjuvants in the preparation of vaccines, particularly those vaccines useful in the treatment of cancer, are provided. Methods for inhibiting tumor growth in an animal are also disclosed. Methods for immunizing an animal against cancer, such as prostate cancer, are also described. The adjuvants described are comprised of an extracellular matrix material, such as small intestinal submucosal (SIS) tissue. The preparations may take the form of sheets, gels, liquids (injectable), trocar, or other solid or semi-solid preparation. The invention provides for enhanced tumor inhibition of 2-fold or greater, compared to vaccine preparations without the extracellular matrix material, or from 4- to 5-fold, compared to preparations without the adjuvant promoting extracellular materials.

The invention provides a preparation method for a novel biomimetic matrix type biological wound healing material. According to the method, small intestinal submucosa with a three-dimensional scaffold structure is compounded with platelet-rich blood plasma containing a specific proportion of bioactive factors, and a specific process is adopted to carry out modification to form the novel material containing biological induction factors, wherein the novel material has a three-dimensional biomimetic structure. With the biomimetic matrix type biological wound healing material prepared by the method of the present invention, the healing can be promoted, the physiological repair can be promoted, the pathological repair can be inhibited, and the regeneration repair efficiency and the repair quality of the damaged tissue can be improved. The novel biological wound healing material of the present invention can be used for repairs of various human soft tissues, membranous tissues or cavity walls, and for cavity filling, and can be used as the ideal extracellular matrix biomimetic material and the cell scaffold material for wound repairs of the tissues and the organs, promotion of wound healing, prevention of adhesion and the like.

The invention discloses a composite biological patch based on pig small intestinal submucosa and a preparation method and application thereof. The composite biological patch is characterized in that the accellular pig small intestinal submucosa is compounded with an additive so as to obtain the composite biological patch based on the pig small intestinal submucos; the additive is selected from one or two of growth factors and medicines. The composite biological patch based on the pig small intestinal submucos has the advantages that the composite biological patch is a good biological stent material, the structure component and the space three-dimensional structure are very similar, the good biological compatibility is presented in the clinical experiment, the healing of wounds is promoted, and various tissues are regenerated and remolded; the biological stent material has effective restoration effect on the healing of skin wounds, and has important meaning on the regeneration of the skins.

The invention relates to a preparation method of an anti-infectious lower biomaterial of small intestinal mucosa. The method comprises the following steps: primarily treating; degreasing; inactivatingvirus; decellularizing; loading antibacterial components; forming; freezing drying or soaking in a storing solution; packing; sterilizing. The lower biomaterial prepared according to the method is good in decellularizing effect; the damage to the structure of a raw material is reduced; an SIS material subjected to decellularizing is complete and uniform in a collagenous fiber structure by observing through a light microscope; the collagenous fiber beam is complete; extremely few cells are residual; complete cells cannot be observed; the collagenous fibers of the material are of three-dimensional net shaped structures by being observed under a scanning electron microscope, and cells are not observed, and few broken cell pieces are residual; after the antibacterial components are loaded tothe SIS, the antibacterial effect is obvious; the clinical demand can be met.

The invention discloses a method for extracting sodium heparin and co-producing amino acid, comprising the following steps: adding refined salt accounting for 1-1.8 weight percent of an intestinal mucosa into small intestinal mucosa; regulating the pH value of a solution to 9.0-9.2; adding fresh porcine pancreas to carry out zymolysis and filtering; carrying out resin absorption, elution and deposition; and drying to obtain the sodium heparin crude product; adding parenzyme into a filtering liquid, keeping the temperature to carry out the zymolysis and filtering to obtain an amino acid solution. The method for extracting sodium heparin and co-producing amino acid co-produces the amino acid solution on the basis of extracting the sodium heparin, thereby improving the economic effect, reducing the waste liquid and lightening the environment pollution.

The invention discloses a liquemin bullion producing method, which comprises the following steps: extracting small intestine mucosa liquid in the reactor tank; using industrial alkali to adjust pH value between 9.5 and 10.5; adding 3-5 percent NaCl; putting enzyme 10 g per 100 bars; heating until 56-81 deg.c; stirring 1-3 min to produce sediment; collecting solidity through physical or chemical method; condensing the supernatant disebrin through micro-filter membrane condenser or resin; collecting one twentieth bulk to primary supernatant; dehydrating; drying; adding 85-90 alcohol in the condensed liquid to 40-50 degree; drying the sediment to produce liquemin bullion.

The invention relates to a heparin sodium technique, in particular to a heparin sodium extracting technique utilizing multi-frequency combination and ultrasonic double-enzyme reinforcement. Firstly, the scraped small intestinal mucosa is sucked in a conventional enzymolysis vessel, and refined salt with a proportion of 2.3 percent to 2.8 percent by weight is firstly added thereto before heating, and the pH value is adjusted to 9.0 to 9.2; secondly, when the solution obtained from the first step is heated to 40 DEG C to 45 DEG C, trypsase and lipase are added, and the proportion is maintained between 10 : 0.5 to 10 : 5, the weight proportion of the double enzyme in the solution in the first step accounts for 0.3 percent to 0.4 percent, the solution is continuously heated to 50 DEG C to 55 DEG C, the enzymolysis under heat preservation is performed for 2 hours to 5 hours, then the solution is continuously heated to 80 DEG C to 85 DEG C, and the solution is filtered after being insulated for 10 minutes to 20 minutes; and thirdly, the filtered solution obtained from the second step is treated through the conventional steps of adsorbing, washing, eluting, precipitating, dehydrating and drying, so as to obtain the heparin sodium product. Compared with the prior art, the invention has the advantages that: firstly, the entire procedure time is substantially reduced by 33 percent; secondly, the yield of products is increased by 29.6 percent; and thirdly, the titer is stabilized to be higher than 120 (the titer of the domestic advanced technology product is between 90 and 110).

A diverticular remodeling technique comprises the introduction of a remodeling agent into the diverticulum to promote permanent closure of the diverticular sac. In disclosed exemplary embodiments, the diverticulum is accessed non-invasively and optionally cleaned to remove unwanted matter therefrom. The remodeling agent is introduced into the diverticular interior and the neck of diverticulum is closed (as necessary) to prevent egress of the remodeling agent. The remodeling agent can be any of a variety of materials, including small intestine submucosa (SIS) collagen or other absorbable fibrosis materials in gel, liquid or solid form. An endoscopic device may be used that includes a multi-lumen flexible catheter having a distal end adapted to be steered through a patient and a proximal end that remains outside the patient's body.

The invention discloses a preparation method of porcine small intestine antibacterial peptides PR39, which comprises the following specific steps of: 1, taking porcine small intestinal mucosa and flushing; 2, adding a mixture of acetic acid and ice water with the volume fraction of 4-5%, and smashing to be made into a homogenate; 3, carrying out a boiling water bath for 10min, and cooling to 0-10 DEG C; 4, centrifuging, cracking a supernatant by ultrasonic waves for 30s, stirring and extracting, centrifuging, and taking the supernatant; 5, mixing a precipitate with acetic acid with the volume fraction of 4-5%, stirring and extracting, centrifuging, and taking the supernatant; 6, combining the supernatants, centrifuging and taking the supernatant; 7, sampling the supernatant to a Sephadex G2100 gel column for chromatography, and collecting an eluate; and 8, detecting the antibacterial property of liquid in each collection tube by an agarose diffusion method, collecting the eluent with an antibacterial effect to obtain the porcine small intestine antibacterial peptides PR39, and storing by freezing and drying in a vacuum. The process is simple, the cost is low, most of impure proteins are removed, and the extraction rate is high.

The invention discloses a method for preparing a biological nanopatch constructed by astragaloside induced BMSC compound polylactic acid-glycolic acid nano-particle modified small intestinal submucosa matrix. The method comprises the following steps: preparing polylactic acid-glycolic acid nano-particles (PLGA) by adopting a multiple emulsion method; preparing a small intestinal submucosa matrix (SIS) having a natural three-dimensional ultra micro-structure by adopting a decellation technology to adsorb the PLGA nano-particles on the surface of SIS and favor adsorption and growth of host cells by means of the SIS after PLGA nano-particle surface modification; inducing BMSC by astragaloside to promote proliferation and differentiation of BMSC and enable the BMSC to compound and grow on the PLGA nano-particle surface modified small intestinal submucosa matrix (PLGA-SIS) of pigs. Therefore, the AS-BMSC-PLGA-SIS biological patch has stable physicochemical property, good biocompatibility and high mechanical strength, and is beneficial to tissue regeneration.

The utility model relates to a small intestinal mucosa scraping device, and relates to an intestinal scraping machine. A rectangular structure is enclosured by two horizontal bars and two longitudinalbars, one horizontal bar has a plurality of fixing clamps, and each longitudinal bar is externally provided with a ring sleeve; the two horizontal bars are connected with both ends of a ball screw, the ball screw is connected with a stepping motor, and the stepping motor is connected with the horizontal bars; the ball screw has a ball screw mother, and rectangular frames exist between the ball screw mother and the two ring sleeves; the inner bottom face of each rectangular frame is connected with a lower scraper, the inner top face of each rectangular frame is connected with an upper scraperthrough a spring, and the upper scrapers are matched with the lower scrapers correspondingly. Through holes are formed in the middle part of the upper end of each rectangular frame, and thread holes corresponding to the through holes are formed in the middle of each upper scraper; the lower ends of limit screws separately interstitially pass through the corresponding through holes and then are connected with the corresponding thread holes in a threaded manner. The problem that the prior art cannot simultaneously scrape mucosa and maintain intestinal integrity is solved.

The invention relates to an otology biological material which can be used for repairing ear wounds including ear drum membrane injury. The material is prepared in the mode that young stock tissue suchas small intestinal submucosa of weaned piglets is used, and tender tissue of young stock is subjected to decellularization under the low-temperature condition through mild plant-derived reagents such as saponin. According to the obtained otology biological material, an ECM three-dimensional structure of the otology biological material is complete, the content of bioactive components is obviouslyhigher than that of an existing product, especially the content of hyaluronic acid is high, cell chemotaxis, growth and proliferation can be better promoted, and otology wound surface healing is facilitated; meanwhile, the otology biological material is easy to degrade and absorb and has an anti-infection effect; the defects of few active ingredients, much ECM structure damage, weak healing promoting capability and slow degradation of the existing product are overcome; and in addition, the dosage form of the otology biological material can be further made into different shapes such as powder,and thus use is more flexible, even simpler, more convenient and painless.

The invention discloses a method for producing heparin sodium by using a membrane and a resin column, and belongs to the technical field of heparin sodium production. In order to solve the problems oflong consumed time, low product yield, difficult treatment of enzymatic hydrolysate and the like in the existing heparin sodium enzymolysis process, the method comprises the following steps: carryingout enzymolysis on small intestinal mucosa of pigs, coarsely filtering the enzymatic hydrolysate, and then filtering by using a microfiltration membrane to obtain intestinal residues, trapped fluid and dialysate; filtering the dialysate by using a nanofiltration membrane to obtain trapped fluid and dialysate; adsorbing the trapped fluid by using resin, and washing and desorbing the resin by usingsaline water after adsorption to obtain adsorbed residual liquid, washing liquid and desorbed liquid; filtering the desorbed solution by using a nanofiltration membrane to obtain trapped fluid and dialysate; and precipitating the trapped fluid with ethanol, collecting the precipitate, dehydrating, and drying to obtain heparin sodium. According to the method, heparin is adsorbed faster and more, adsorption adopts a resin column for adsorption, and compared with adsorption of an adsorption tank, the method has the advantages of less resin consumption, short adsorption time and high adsorption rate.

The present invention provides a degradable spherical artificial bladder, which is a medical in situ implantable degradable spherical artificial bladder lined with small intestinal submucosa. The artificial ureteral loop and the artificial urethral loop are reticular pipe structures, and the artificial bladder body is a reticulated spherical structure. The artificial urethral loop, the artificial ureteral loop, and the artificial bladder body are integrally formed, and the small intestinal submucosa is lined inside the three. The invention has a reasonable structural design and fully utilizes the advantages of natural-artificial composite materials. The mesh outer support plays a role of mechanical support in the early stage of implantation, facilitates the suture operation of the surgical port, and can maintain the structure of the urinary bladder for temporary urine storage. functions as well as facilitate fixation to pelvic tissues. The invention is applied in cystectomy operation to replace the resected diseased bladder, and finally form an organ similar to the autologous bladder.

The invention discloses an orally-taken tuckahoe polysaccharide sulfate-chitosan nanoparticle and a preparation method thereof. The preparation method comprises the following steps of: slowly adding a tuckahoe polysaccharide sulfate solution into a chitosan solution with stirring and continuously stirring, wherein at the same time, a mixed solution becomes turbid and a stable nano colloid solution is obtained; and spraying and drying the nano colloid solution by adopting a spraying and drying method at the high temperature of 70-120DEG C to obtain the orally-taken tuckahoe polysaccharide sulfate-chitosan nanoparticle. The nano-particle prepared by the preparation method is high in drug-loading rate, is small in particle size and is high in stability under the acidic environments of which the pH value is smaller than 7.5 and the temperature is about 37DEG C; and the nano-particle can be affined with small intestine and penetrates through the small intestine at the duodenum part in a form of complete nanoparticle and the medicament slowly releases in the blood.

The present invention relates to a method for preparing a biocompatible small intestinal submucosa hydrogel with a controllable in-vivo degradation period, more particularly to a method for preparing a small intestinal submucosa hydrogel which is formed in a solution phase by forming chemical crosslinkages by solubilizing a biocompatible small intestinal submucosa powder and allows the degradation period of a gel formed during in-vivo injection by controlling the degree of crosslinking and a method for preparing a small intestine submucosa drug carrier or a small intestine submucosa support using the hydrogel.

The invention discloses a Chinese herbal compound for preventing and treating porcine epidemic diarrhea virus, comprising: preparing Herba Phyllanthi Urinariae, Herba Origani Vulgaris and Herba Salviae Plebeiae into powder according to a weight ratio of 3:2:1, mixing the powder and feed according to a weight ratio of 1:100, and feeding a diseased pig; or adding distilled water 10 times as heavy as the powder, soaking for 1 h before 1 h of heating and refluxing, filtering with gauze while temperature is high, adding water 8 time as heavy as residue and water 6 times as heavy as the residue respectively to the residue for 1 h of heating and refluxing, combining three filtrates, concentrating under reduced pressure to obtain liquid medicine 1 g / mL in concentration, and intramuscularly injecting the diseased pig. TFF3 content can be significantly increased, thus improving mucosal defensive function of animal small intestines, decreasing NO level, improving intestinal peristalsis and intestinal juice electrolyte balance, enhancing animal physique, and decreasing bodily urea level can be decreased; ALB level can be significantly increased to improve bodily liver metabolism, GLO level can be significantly increased to enhance body immunity.

The invention provides a synchronous separation-extraction technique for an intestinal mucous byproduct, relating to the technical field of biological pharmacy and feed production. The synchronous separation-extraction technique comprises the following steps: firstly extracting heparin sodium through salt decomposition, separating heparin from proteins, and extracting crude product heparin; and activating proteins produced during the production by virtue of fresh pancreas, and carrying out enzymolysis, so as to produce protein peptide. The product contains rich small peptide easily digested and absorbed by livestock and is a novel animal derived feed protein rich in short peptide chains and balanced amino acids. By utilizing the product, the production performance and health level of animals, particularly young animals, can be enhanced; and amino acids existing in a short peptide manner and free amino acids have relatively high biological activities and can be taken as protein feed sources of multiple animals.

The invention discloses a method for preparing an oral patch of composite oral mucosal epithelial cells, which comprises the following steps: preparing the oral patch by using the submucosa of the small intestine, cultivating the oral mucosal epithelial cells by a subculture method, and inoculating the oral mucosal epithelial cells into the oral cavity The patched cell culture dishes were cultured at 37°C in a 5% CO2 incubator. After the culture was terminated, the oral patch of composite oral mucosal epithelial cells was obtained. The preparation method of an oral patch of composite oral mucosal epithelial cells provided by the present invention has sufficient material sources, can make oral mucosal epithelial cells proliferate in large quantities in the oral patch, and provides a new method for the repair and reconstruction of large-area defects in the oral mucosa in clinical practice. way.

The invention discloses a preparation method of lithium heparin, porcine small intestinal mucosa is used as a raw material for extracting a refined heparin sodium product, and ion exchange resin is used for realization of exchange of a heparin sodium salt and a heparin lithium salt; the preparation method comprises the following steps: preparation of the refined heparin sodium product, lithium exchange and freeze drying. According to the preparation method, after the porcine small intestinal mucosa is used as the raw material for extracting the refined heparin sodium product, the ion exchange resin is used for realization of the exchange of the heparin sodium salt and the heparin lithium salt; raw material sources are wide, and the preparation cost is low.

The invention provides a method for infecting a porcine small intestinal mucosa epithelial cell line with porcine epidemic diarrhea virus, and belongs to the technical field of biotechnology. The method comprises the following steps of mixing small intestinal mucosa epithelial cells with porcine epidemic diarrhea virus liquid, performing adsorbing, removing the liquid, mixing a left substance witha liquid culture medium, and carrying out culturing so as to achieve infection of porcine small intestinal mucosa epithelial cell line by the porcine epidemic diarrhea virus. By adopting the method for culturing the porcine epidemic diarrhea virus in vitro provided by the invention, the porcine epidemic diarrhea virus can be cultured in vitro on the porcine small intestinal mucosa epithelial cellline, and the problem that the porcine epidemic diarrhea virus is difficult to culture on an in vitro target cell is solved; in addition, the copy number of the virus is increased from 106.26 copies / [mu]L to 107.51 copies / [mu]L in the culture process, so that a proper biological material is provided for researching the pathogenesis of the porcine epidemic diarrhea virus.

A biological material with composite extracellular matrix component, in which decellularized small intestinal submucosa (SIS) is treated as the interlayer and decellularized urinary bladder matrix (UBM) is treated as superior and inferior surface layers. The interlayer is totally encapsulated by the mentioned superior and inferior surface layers, forming a sandwich structure with advantages of integrating UBM and SIS to have high bioactivity with bionic structure, UBM isolates the immunogenicity of SIS and direct contact with host tissue, and after implantation the basic type of inflammatory interaction in the host-implant marginal zone is the same as that of pure UBM, with high biocompatibility; effective endotoxin removal optimize the biosafety of the material after implantation; feasibility for industrial large-scale production; the stiffness of the material can be maintained even after hydration, with good handling feel and fit condition, beneficial for the suture fixation and also shorten the fixation or surgery time.

The invention provides a method for preparing crude heparin sodium from porcine small intestine mucosa with low salt. The method comprises the following steps: carrying out low-salt composite enzymolysis to obtain an enzymatic hydrolysate, concentrating the enzymatic hydrolysate to obtain a concentrated solution, carrying out ion exchange to obtain a crude heparin sodium eluent, carrying out alcohol precipitation, and performing drying to obtain the crude heparin sodium, to be specific, adding saturated casingsaline water in thelow-salt composite enzymolysis; and adjusting the salt concentration of the mucous membrane solution to 0.8-1.0% by mass. According to the method for preparing the crude heparin sodium from the porcine small intestine mucous membrane in the low-salt mode, enzymolysis and adsorption processes of the crude heparin sodium can be achieved under the low-salt condition, the salt concentration in discharged wastewater can be reduced while the yield of the crude heparinsodium is guaranteed, the wastewater treatment cost is reduced, environmental pollution is reduced, and comprehensive economic benefits are increased.

The invention discloses an application of notoginsenoside R1 in preparation of drugs for treating small intestinal mucosal injury, intestinal inflammatory cell infiltration and increase in microvessel permeability caused by ischemia / reperfusion. The application is realized by a way that the notoginsenoside R1 inhibits I[kappa]B[alpha] degradation and metastasis of NF-[kappa]BP65 translocates to cell nuclei, and then inhibits inflammatory cytokine release; the application is realized by a way that the notoginsenoside R1 inhibits leukocytes to swim out of microvessels, so as to avoid infiltration in tissues; the application is realized by a way that the notoginsenoside R1 inhibits cell apoptosis; the application is realized by a way that the notoginsenoside R1 inhibits degradation of vascular intercellular tight-junction proteins and inhibits plasma albumin leakage; and the application is realized by a way that the notoginsenoside R1 regulates ATP5D, increases the content of ATP in intestinal canal tissues, and promotes repair of intestinal mucosa epithelial cells.

The invention belongs to the technical field of chicken cultivation and in particular relates to a feeding method capable of improving growth performance of broiler chicken 1-21 days old. The feeding method comprises the following concrete steps: (1) feeding young chicken with feed everyday, namely feeding the young chicken with feed for five times everyday; and (2) feeding the young chicken with nutrient solution everyday, namely feeding the young chicken with the nutrient solution twice everyday. The feeding method provided by the invention has the advantages that content of proteins, DNA and RNA of intestinal mucosae is increased, intestinal villi are heightened, intestinal walls are thickened, small intestinal mucosa enzyme activity is improved, intestinal development is further promoted, growth of the intestinal villi is promoted, villi are heightened, and recesses are shallowed, so that quantity of mature cells of intestinal epitheliums is increased, and maturation rate is increased, capability of digesting and absorbing nutrient substances is enhanced, feed utilization rate is improved, and the broiler chicken can be grown rapidly; meanwhile, metabolism of microorganisms is accelerated, structure of a flora is adjusted, intestinal microecological balance is effectively maintained, intestinal conditioned pathogens are inhibited, nutrition is provided, body immunity is enhanced, and robust growth of the young chicken is promoted.

The invention discloses a method for purifying a crude product of heparin sodium. The method adopts the small intestinal mucosa of a healthy pig as a main raw material and obtains a crude product of heparin sodium by the steps of dissociation (enzymolysis), filtration, protein enzymolysis, protein removal and precipitation, dehydration and drying. The method has the advantages of short production cycle, low production cost and high yield, does not generate toxic substances, and is good for environmental protection.

The invention discloses a skin injury repair material. A small intestinal mucosa lower membrane serves as a substrate and is compounded with adipose mesenchymal stem cells to form the repair material.The repair material is good in repair effect and clinical application prospect.

The invention provides a method for preparing copper / zinc mesoporous biological glass-loaded small intestinal submucosa wound surface dressing through a low-temperature three-dimensional printing technique. The method is characterized by comprising the following steps: (1) preparing a small intestinal submucosa (SIS) matrix material; (2) preparing copper and zinc mesoporous biological glass (Cu-MGB / Zn-MGB); (3) compositing SIS with Cu-MGB / Zn-MGB; (4) preparing a wound surface dressing based on the low-temperature three-dimensional printing technique; and (5) carrying out after-treatment process. The SIS wound surface dressing has the advantages that the SIS wound surface dressing has excellent efficacies of resisting bacteria, stopping bleeding, promoting vascularization and accelerating the regeneration rate of the wound surface; the customization design and production can be realized according to different clinic demands; the cost is low, the manufacturing process is stable and controllable, and the large-scale production can be realized; and the wound surface dressing can be stored for a long time and is convenient to use.