Preparation method of anti-infectious lower biomaterial of small intestinal mucosa
A technology of small intestinal submucosa and biomaterials, applied in medical science, prosthesis, etc., to achieve the effect of enhancing digestion
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[0059] The invention provides a method for preparing an anti-infection small intestinal submucosa biological material, comprising the following steps:
[0060] 1) Initial treatment: take the small intestine of the pig within 5 hours after slaughter, cut it into sections, each section is 3-30 cm long, then cut it into slices along the central axis, and rinse it repeatedly with clean water (no requirement, subjective judgment is enough) ), using mechanical methods to remove the mucosal layer, muscular layer and serosal layer to obtain submucosal tissue SIS, and then rinse with water for 5-10 times;
[0061] 2) Degreasing: The submucosa tissue obtained in step 1) is degreased with a degreasing agent for 30-90 minutes, and after degreasing, rinse with water for 5-10 times.
[0062] In the above step 2), the mixed degreasing agent can be composed of a trisodium phosphate solution with a mass concentration of 1-3% and a surfactant with a mass concentration of 0.1-1.0%; the trisodium...
Embodiment 1
[0086] This embodiment provides a method for preparing an anti-infection SIS biomaterial, comprising the following steps:
[0087] Primary treatment: take the small intestine of the pig within 5 hours after slaughter, cut it into small sections with a length of 8 cm, cut it longitudinally into slices, wash it repeatedly with clean water, remove the mucosal layer, muscular layer and serosa layer by mechanical means, and obtain the submucosa tissue of the small intestine SIS; rinse with water 5 times, 20 minutes each.
[0088] Degreasing: use a mixed degreasing agent consisting of 1% sodium bicarbonate solution, 3.5% sodium hydroxide solution and 0.5% Triton X-100 for 30 minutes. After degreasing, rinse with water 10 times, 3 minutes each time. The mass ratio of SIS to mixed degreasing agent is 1:5.
[0089] Virus inactivation: use a virus inactivation solution composed of tributyl phosphate solution with a mass concentration of 0.3% and Tween 80 with a mass concentration of 2...
Embodiment 2
[0097] This embodiment provides a method for preparing an anti-infection SIS biomaterial, comprising the following steps:
[0098] Initial treatment: take pig small intestine within 5 hours after slaughter, cut it into 6 cm long pieces, cut it longitudinally into slices, wash it repeatedly with clean water, remove the mucosal layer, muscular layer and serosal layer by mechanical means, and obtain the submucosa tissue SIS ; Rinse with water 10 times, 10 minutes each time.
[0099] Degreasing: using a mixed degreasing agent consisting of 5% sodium bicarbonate solution, 1.5% sodium hydroxide solution and 1.0% Triton X-100 for 90 minutes. After degreasing, rinse with water 5 times, 8 minutes each time. The mass ratio of SIS to mixed degreasing agent is 1:10.
[0100]Virus inactivation: use a virus inactivation solution composed of tributyl phosphate solution with a mass concentration of 0.5% and sodium cholate with a mass concentration of 0.5%, soak for 10 hours to inactivate th...
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