The invention relates to a method for biological detoxication of pretreated lignocellulose biomass, which comprises the following steps: step one, performing pretreatment for preparation of raw materials; and step two, performing biological detoxication and fermentation: inoculating fungi Amorphotheca resinae ZN1 by using PDA as a medium, followed by cultivation at a temperature of 20 to 30 DEG C; performing elution to obtain A. resinae ZN1 spores, inserting the A. resinae ZN1 spores into the pretreated lignocellulose biomass raw materials, and carrying out cultivation until the growing of mycelia, so that a first order seed is obtained; and inserting the first order seed into the raw materials, wherein the water content is 10 to 90%, adjusting the pH of the raw materials to 4 to 7, and then carrying out cultivation for 1 to 7 days at a temperature of 20 to 40 DEG C. The method for biological detoxication of the pretreated lignocellulose biomass provided in the invention has advantages of simple operation and low requirement for equipment. Furthermore, various mainly inhibitors produced during the pretreatment processing can be degraded. Therefore, the continuity of simultaneous saccharification and fermentation of high-solid content can be ensured, processing steps can be reduced, and working procedure time can be shorten and working procedure cost can be reduced.