Preparation method of compound oral patch for oral mucosal epithelial cells

Abstract

The invention discloses a method for preparing an oral patch of composite oral mucosal epithelial cells, which comprises the following steps: preparing the oral patch by using the submucosa of the small intestine, cultivating the oral mucosal epithelial cells by a subculture method, and inoculating the oral mucosal epithelial cells into the oral cavity The patched cell culture dishes were cultured at 37°C in a 5% CO2 incubator. After the culture was terminated, the oral patch of composite oral mucosal epithelial cells was obtained. The preparation method of an oral patch of composite oral mucosal epithelial cells provided by the present invention has sufficient material sources, can make oral mucosal epithelial cells proliferate in large quantities in the oral patch, and provides a new method for the repair and reconstruction of large-area defects in the oral mucosa in clinical practice. way.

Description

technical field [0001] The invention relates to a method for preparing an oral patch made of composite oral mucosa epithelial cells, belonging to the field of cell biology technology. Background technique [0002] Oral mucosa, as the first line of defense of the human body's biological barrier, plays a vital role in daily life, but the location of the oral mucosa and its surrounding environment lead to a variety of factors that can cause loss of oral mucosal tissue, such as oral and maxillofacial surgery Tumor resection, trauma, and alveolar surgery before repair often have large oral mucosal defects, which are often repaired clinically by skin grafting or flap transfer, but skin grafting often causes oral cavity narrowing and affects function due to contracture, and local flaps Metastasis often causes local swelling and poor shape, which is difficult for patients to accept. Moreover, autologous tissue transplantation is a treatment mode of "repairing damage by trauma" at...

AI Technical Summary

Problems solved by technology

[0002] Oral mucosa, as the first line of defense of the human body's biological barrier, plays a vital role in daily life, but the location of oral mucosa and its surrounding environment lead to a variety of factors that can cause loss of oral mucosal tissue, such as oral and maxillofacial surgery Tumor resection, alveolar surgery before trauma and repair often have large oral mucosal defects, which are often repaired clinically with skin grafts or flap transfers, but skin grafts often cause oral cavity stenosis and affect function due to contracture, and local flaps Metastasis often causes local swelling and poor shape, which is difficult for patients to accept

Moreover, autologous tissue transplantation is a treatment mode of "repairing damage by trauma" at the expense of normal tissue, which not only increases new trauma, but also increases the probability of surgical complications, and the limited source of autologous tissue limits its clinical application

In recent years, with the rise of biomaterials, clinical oral mucosal defect repair has achieved relatively satisfactory therapeutic effects, but for large-area oral mucosal defects, there are still major limitations, and it is difficult to achieve the expected therapeutic effect