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Porous decellularized tissue engineering cartilage support and preparation method thereof

A tissue engineering and decellularization technology, applied in the field of medical materials, can solve the problems of low initial mechanical strength and inability to completely simulate the performance of natural cartilage, and achieve good biomechanical properties, easy operation and simple technical requirements

Active Publication Date: 2015-03-11
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its initial mechanical strength is low, and the phenomenon of "hollow" appears in the process of compound cell culture, which cannot completely simulate the properties of natural cartilage.

Method used

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  • Porous decellularized tissue engineering cartilage support and preparation method thereof
  • Porous decellularized tissue engineering cartilage support and preparation method thereof
  • Porous decellularized tissue engineering cartilage support and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] (1) Take the cartilage of the femoral condyle of fresh adult porcine, remove the surrounding tissue, wash with a sterilized distilled water spray gun under high pressure, and remove blood components and adipose tissue as much as possible.

[0039] (2) The obtained cartilage was made into 5 cylindrical cartilage slices with a diameter of 4 mm and a thickness of 2 mm (see figure 1 ).

[0040] (3) After being stored at -24°C for 24 hours, it was frozen in a -80°C refrigerator for 72 hours and then taken out.

[0041] (4) Soak the prepared tissue engineered cartilage slices in 0.01MPBS buffer solution, and drill holes on the soaked cartilage slices with a carbon dioxide laser puncher. The hole diameter is 300 μm, the hole spacing is 300 μm, and the porosity is 85%.

[0042] (5) After drilling, rinse and soak the porous tissue engineered cartilage sheet prepared in step (4) with sterile three-distilled water, freeze at -20°C for 24 hours, then transfer to -80°C for 72 hours...

Embodiment 2

[0046] Frozen sections of the prepared scaffolds were stained with Hoechst 33258 (see figure 2 ), without nuclear staining, indicating that the cartilage slices were successfully decellularized; HE staining (see image 3 ), toluidine blue staining (see Figure 4 ), showing the microstructure of the tissue engineered cartilage scaffold; scanning electron microscopy shows the internal structure of the tissue engineered cartilage scaffold (see Figure 5 ).

[0047] The prepared tissue engineered cartilage scaffold and the cartilage tissue of the same size were subjected to biomechanical tests, and the compression elastic modulus of the prepared cartilage scaffold was 0.5734 ± 0.021Mpa, which was statistically different from normal articular cartilage (0.6875 ± 0.013Mpa) ( *P Image 6 ).

[0048] Inoculate mouse fibroblast L929 in a 96-well plate, and set up three groups, namely negative control group, test group, positive control group, normal culture medium was added to the n...

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Abstract

The invention discloses a porous decellularized tissue engineering cartilage support, which is prepared through a decellularized method after tissue engineering cartilage materials are subjected to punching treatment, so as to achieve three-dimensional porosity, higher biomechanical property and biocompatibility. The tissue engineering cartilage support is applicable to defect repair of cartilages in loaded parts, and can become a major breakthrough in defect repair of cartilages in tissue engineering.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a novel tissue engineering cartilage support and a preparation method. Background technique [0002] Articular cartilage damage caused by trauma, inflammation, degeneration, tumor resection, etc. is a common clinical disease. The ability of articular cartilage to regenerate and repair is extremely limited. Once damaged, it is difficult to repair itself, and the disease will continue to develop, which will inevitably lead to osteoarthritis. The current treatment methods, such as microfracture, autologous or allogeneic periosteum, perichondrium, and osteochondral block transplantation, all have various defects, which limit the clinical application. With the emergence of tissue engineering technology in the nineteenth century, the application of tissue engineering technology to construct tissue-engineered cartilage or osteochondral complexes is considered the best technic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/36
Inventor 彭江徐小龙王玉王程卢世璧许文静汪爱媛郭全义
Owner GENERAL HOSPITAL OF PLA
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