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Extraction and preparation method by comprehensively utilizing liquorice

A technology of licorice and glycyrrhizic acid, which is applied in the field of preparation of isoliquiritigenin and glycyrrhizinic acid, can solve the problems of toxic organic solvent isoliquiritigenin product quality, damage to glycyrrhizic acid components, increase production costs, etc., to save traditional Chinese medicine resources and improve The effect of comprehensive utilization and low cost

Active Publication Date: 2012-08-15
XINJIANG TEFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The same problems brought about by these two schemes are: ① Since the licorice extract contains licorice flavonoids (mainly glycyrrhizin) and glycyrrhizic acid components, glycyrrhizic acid will also be hydrolyzed to form glycyrrhetinic acid during the hydrolysis of licorice flavonoids , which makes the glycyrrhizinic acid component destroyed and unusable; ②Glycyrrhetinic acid produced after acid hydrolysis of glycyrrhizic acid has a low polarity, which is very close to the polarity of isoliquiritigenin. Therefore, it is often necessary to use Normal phase chromatography such as silica gel column chromatography, while greatly increasing production costs, the use of toxic organic solvents also affects the quality of isoliquiritigenin products

Method used

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  • Extraction and preparation method by comprehensively utilizing liquorice
  • Extraction and preparation method by comprehensively utilizing liquorice
  • Extraction and preparation method by comprehensively utilizing liquorice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Take 5kg of licorice, add water to decoct twice, add water to 8 times the weight of the medicinal material for the first time, decoct for 2 hours, add water for the second time to 6 times the weight of the medicinal material, decoct for 1 hour, combine the decoction, filter, and filtrate Concentrate under reduced pressure at 0.07MPa and 80°C to 2.5L (the amount of crude drug is 0.2g / mL), add ammonia to adjust the pH to 6.0, and pass through 80-100 mesh polyamide (dry weight: 2.5kg) chromatographic column and AB-8 Macroporous resin (dry weight is 2.5kg) chromatographic column;

[0069] Preparation of licorice polysaccharide: collect the effluent, concentrate to 65°C when the relative density is 1.10, add 95% (v / v) ethanol to make the alcohol content reach 80% (v / v), stir, let stand for 12 hours, filter and dry , to obtain licorice polysaccharide 206g, the content is 56.2% (anthrone sulfate colorimetric method);

[0070] Preparation of isoliquiritigenin: The polyamide ch...

Embodiment 2

[0073] Take 5kg of licorice, reflux extraction twice, add 8 times the amount of 50% ethanol for the first time, reflux extraction for 2 hours, add 6 times the amount of 50% ethanol for the second time, reflux extraction for 2 hours, combine the extracts, filter, and the filtrate is at 0.12MPa , Recover ethanol under reduced pressure at 60°C, add water to 2.5L (crude drug amount 0.2g / mL), add 10% sodium hydroxide solution to adjust pH to 7.0, and pass through 80-100 mesh polyamide (dry weight: 10kg) chromatography Column and AB-8 macroporous resin (dry weight is 10kg) chromatographic column;

[0074] Preparation of licorice polysaccharide: collect the effluent, concentrate to 65°C when the relative density is 1.15, add 95% (v / v) ethanol to make the alcohol content reach 80% (v / v), stir, let stand for 24 hours, filter and dry , to obtain 91g of Glycyrrhiza polysaccharide, the content of which is 63.8% (anthrone sulfate colorimetric method);

[0075] Preparation of isoliquiritig...

Embodiment 3

[0078] Take 5kg of licorice, add water to decoct twice, add water to 8 times the weight of the medicinal material for the first time, decoct for 2 hours, add water for the second time to 6 times the weight of the medicinal material, decoct for 1 hour, combine the decoction, filter, and filtrate Concentrate under reduced pressure at 0.10MPa and 70°C to 5L (the amount of crude drug is 0.1g / mL), add ammonia to adjust the pH to 6.0, and pass through 80-100 mesh polyamide (dry weight: 5kg) chromatographic column and AB-8 macroporous Resin (dry weight is 5kg) chromatographic column;

[0079]Preparation of licorice polysaccharide: collect the effluent, concentrate to 65°C when the relative density is 1.15, add 95% (v / v) ethanol to make the alcohol content reach 80% (v / v), stir, let stand for 36 hours, filter and dry , to obtain licorice polysaccharide 183g, the content is 78.6% (anthrone sulfate colorimetric method);

[0080] Preparation of isoliquiritigenin: The polyamide chromatog...

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PUM

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Abstract

The invention discloses an extraction and preparation method by comprehensively utilizing liquorice and belongs to the technical field of traditional Chinese medicine. Isoliquiritigenin, glycyrrhizic acid and liquorice polysaccharide which have high additional values are separated and prepared by a polyamide-macroporous resin coupling technique. The extraction and preparation method has the advantages of simple technical route and green and safe production process, does not need to use toxic and organic solvents and is suitable for mass production, the comprehensive utilization degree of the liquorice is greatly improved, the traditional Chinese medicine resource is saved, and good social benefit and economic benefit are generated.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a method for simultaneously extracting and preparing licorice polysaccharide, isoliquiritigenin and glycyrrhizic acid from licorice. Background technique [0002] Licorice is the root and rhizome of Glycyrrhiza plants in the family Leguminosae. There are 29 species and 6 varieties in the world, of which 18 species and 3 varieties are produced in China, mainly distributed in North China (including Inner Mongolia and Hebei) and Northwest China (including Ningxia, Gansu, Xinjiang, Qinghai, Shaanxi). As a medicinal and ecological plant, licorice resources are becoming more and more valuable, so the comprehensive utilization of licorice is particularly important. [0003] Modern chemical research shows that licorice mainly contains flavonoid glycosides (liquiritin, isoliquiritin, etc.) -5%) had the highest content. In addition, licorice also contai...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07C49/835C07C45/61C07J63/00C07H15/256C07H1/08A61P29/00A61P31/12A61P37/08A61P37/02A61P39/06A61P35/00A61P9/06
Inventor 郑云枫魏娟花彭国平
Owner XINJIANG TEFENG PHARMA
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