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Application of isoliquiritigenin as cancer-differentiating inducer

A technology of differentiation inducer and isoliquiritigenin, which can be applied in the field of medicine and can solve problems such as adverse reactions

Inactive Publication Date: 2010-01-20
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cytotoxic antineoplastic drugs widely used in clinical practice can kill rapidly proliferating malignant tumor cells and also kill rapidly proliferating normal tissue cells in the body, resulting in serious adverse reactions.

Method used

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  • Application of isoliquiritigenin as cancer-differentiating inducer
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  • Application of isoliquiritigenin as cancer-differentiating inducer

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0028] Experimental example 1 Isoliquiritigenin induces differentiation of human leukemia cells

[0029] 1.1 Experimental materials

[0030] Isoliquiritigenin was purchased from Jiangxi Bencao Tiangong Technology Co., Ltd., with a purity of 99.8% (as determined by HPLC). HL-60 human promyelocytic acute leukemia cell line was purchased from China Type Culture Collection Center (Wuhan University Collection Center), cultured in DMEM containing 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin base (GIBCO).

[0031] 1.2 Determination of cell viability by MTT method and trypan blue exclusion method

[0032] Take the HL-60 cells in the logarithmic growth phase, stain with Pan blue to count the viable cells, and adjust the cell concentration to 1×10 5 / ml, add 100 μl of cell suspension to each well of a 96-well culture plate, place in 5% CO 2 , and cultured in a 37°C incubator for 24h. HL-60 cells were divided into the following groups: ①Normal control group: cul...

experiment example 2

[0054] Experimental example 2: Isoliquiritigenin induces differentiation of human liver cancer cells

[0055] 2.1 Experimental materials

[0056] Isoliquiritigenin was purchased from Jiangxi Bencao Tiangong Technology Co., Ltd., with a purity of 99.8% (as determined by HPLC). Bel-7402 human liver cancer cell line was purchased from China Type Culture Collection Center (Wuhan University Collection Center), cultured in DMEM medium (GIBCO) containing 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin . AFP immunohistochemistry kit was purchased from Beijing Zhongshan Biotechnology Co., Ltd., alpha-fetoprotein (AFP) and albumin (ALB) radioimmunoassay kits were purchased from China Institute of Atomic Energy, tyrosine-α-ketoglutarate aminotransferase ( TAT) and γ-glutamyl transpeptidase (γ-GT) detection kits were purchased from Shanghai Changzheng Medical Science Co., Ltd.

[0057] 2.2 Determination of cell viability by MTT method and trypan blue exclusion method ...

experiment example 3

[0077] Experimental Example 3: Differentiation of malignant melanoma cells induced by isoliquiritigenin

[0078] 3.1 Experimental materials

[0079] Isoliquiritigenin was purchased from Jiangxi Bencao Tiangong Technology Co., Ltd., with a purity of 99.8% (as determined by HPLC). B16 mouse melanoma cell line, purchased from China Type Culture Collection Center (Wuhan University Collection Center), cultured in DMEM medium (GIBCO) containing 10% fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin At 37°C, 5% CO 2 , a carbon dioxide incubator with saturated humidity.

[0080] 3.2 Determination of cell viability by MTT method and trypan blue tie-staining method

[0081] Take the B16 cells in the logarithmic growth phase, stain with trypan blue to count the viable cells, and adjust the cell concentration to 1×10 5 / ml, take two 96-well culture plates, add 100 μl cell suspension to each well, place in 5% CO 2 , and cultured in a 37°C incubator for 24h. Each well of th...

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Abstract

The invention belongs to the technical field of medicines, and discloses application of isoliquiritigenin to prepare medicaments of inducing a tumor to be differentiated. Tests prove that when the concentration of the isoliquiritigenin is low, the isoliquiritigenin cannot kill the tumor cells, but has the action of reversing the tumor cells in leukemia, liver cancer, malignant melanoma and the like into the cell with normal functions. Based on the characteristics, the isoliquiritigenin is mixed with other medical auxiliary materials to prepare an oral or injectable preparation; when the oral or the injectable preparation is clinically used for treating various malignant tumors, the preparation has the advantages of confirmed curative effect, no obvious toxic and side effects, and the suitability for long-period and continuous medicine-taking; and the medicament prepared by the isoliquiritigenin is obviously better than the prior medicament clinically used for treating the tumors,.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new application of isoflavone compound extracted from licorice plants—isoliquiritigenin, in particular to its application as a cancer differentiation inducer in the preparation of antitumor drugs. Background technique [0002] Isoliquiritigenin (2,4,4-trihydroxychalcone, isoliquiritigenin) is an isoflavone compound isolated and purified from Glycyrrhiza. It can be extracted by supercritical carbon dioxide extraction technology, chloroform extraction, etc. Isoliquiritigenin was separated and purified by pore adsorption resin technology and column chromatography (Northeast Forestry University, Research on Extraction and Purification Technology of Isoliquiritigenin and Glycyrrhizic Acid, 2005). The chemical structure and physical and chemical properties of isoliquiritigenin have been described in detail by the Phytochemistry Laboratory of Shanghai Institute of Materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P35/00
Inventor 王振华李德芳陈红梅付伟刘婷张玉柯李忌郑秋生
Owner SHIHEZI UNIVERSITY
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