62results about How to "Characterizing quality" patented technology

The invention discloses an establishment method of a curcuma aromatica medicine fingerprint map and the fingerprint map thereof, and particularly, provides establishment of a HPLC fingerprint map for controlling the quality of curcuma aromatica medicine. The method includes the steps of: 1) preparation of a reference substance solution: preparing the reference substance solutions of curdione, curcumadiol, procurcumenol; 2) preparation of a sample solution: extracting the curcuma aromatica medicine and filtering an extract liquid; 3) HPLC detection to obtain the fingerprint map: performing HPLC detection with octadecylsilane chemically bonded silica as a filler and an acetonitrile-tetrahydrofuran-0.1% phosphoric acid water system as a mobile phase in a chromatographic column in a gradient elution manner, wherein column temperature is 30 DEG C, ultraviolet detection wavelength is 230-250 nm and time is 60-65 min; and 4) similarity evaluation: introducing the obtained fingerprint map into "fingerprint similarity evaluation system of traditional Chinese medicine chromatography" (2004A version) similarity software for performing similarity evaluation. The method is simple, quick and accurate, has good repeatability, and provides powerful guarantee for comprehensive and effective control on quality of the curcuma aromatica medicine.

The invention discloses a Lanqin oral solution fingerprint spectrum establishment method, a fingerprint spectrum of a Lanqin oral solution and application of the fingerprint spectrum. A test solutionis prepared from the Lanqin oral solution, a reference substance solution is a solution which dissolves adenosine, epigoitrin, phellodendrine chloride, chlorogenic acid, magnoflorine, geniposide, benzoic acid, berberine hydrochloride, baicalin, wogonoside, baicalein and wogonin, and the liquid chromatography is measured by using a high-performance liquid chromatograph; the obtained liquid chromatography is introduced into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system for analysis, and the Lanqin oral solution fingerprint spectrum is obtained after multi-point correction, data matching and analysis. According to the Lanqin oral solution fingerprint spectrum establishment method and the fingerprint spectrum of the Lanqin oral solution, the quality information of the Lanqin oral solution can be comprehensively reflected, and uniform and stable product quality is ensured.

The invention relates to the field of detection methods of active ingredients of functional foods, and discloses a propolis supercritical CO2 extract gas chromatography-mass spectrometer (GC-MS) fingerprint and a construction method thereof. The propolis supercritical CO2 extract GC-MS fingerprint has 8 common peaks, wherein the total length of the fingerprint is 60 minutes. The construction method comprises the following steps of: placing a propolis sample in a supercritical CO2 extraction kettle to extract so as to obtain a supercritical CO2 extract of the propolis; weighing the propolis sample and placing in a sample bottle to perform solid-phase micro-extraction; performing GC-MS analysis; and finally comparing the GC-MS fingerprints of propolis supercritical CO2 extracts in different regions, and extracting the common peaks to form the GC-MS fingerprint of the propolis supercritical CO2 extract. The invention has the advantages that: the fingerprint is convenient and stable, has high precision and repeatability, is easy to master and the like. The time for detecting the sample is short; and the obtained propolis supercritical CO2 extract contains more than 120 volatile components.

The invention discloses a method for establishing a fingerprint spectrum of rhizoma alismatis decoction and the fingerprint spectrum. The method comprises the following steps: (1) preparing rhizoma alismatis decoction by adopting rhizoma alismatis and bighead atractylodes rhizomes with different producing areas and different batch numbers, and extracting rhizoma alismatis water decoction by utilizing a methanol solution to obtain a test solution; dissolving atractylodes macrocephala lactone I, atractylodes macrocephala lactone II, atractylodes macrocephala lactone III, alisol A, alisol B and 23-acetyl alisol B by using a methanol solution to prepare a reference substance solution; and (2), carrying out ultra-high liquid chromatography analysis on the test solution and the reference solution, and recording the chromatogram. The rhizoma alismatis decoction fingerprint spectrum established by the method provided by the invention can effectively characterize the quality of the rhizoma alismatis decoction, and provides a basis for monitoring the quality of medicinal materials. Meanwhile, the method has the advantages of being simple, rapid, stable, good in reproducibility, high in precision and the like.

The invention provides an establishment method of a compound rheum officinale Qingyi decoction fingerprint. The establishment method comprises the following steps: preparation of a sample solution fortest, a single-flavor ingredient medicinal material sample solution for test and a reference substance solution, determination of chromatographic conditions of an HPLC (high performance liquid chromatography), and determination of a fingerprint, wherein the conditions of the HPLC are described as follows: a column is UItimate LP-C18 (250mm*4.6mm, 5mum), the flow velocity is 1.0mL / min, the columntemperature is 30 DEG C, the detection wavelength is 237nm, acetonitrile (A)-0.1% phosphoric acid solution (B) is adopted, and gradient elution is adopted. In the establishment method, the compound rheum officinale Qingyi decoction fingerprint established in 'Traditional Chinese Medicine Chromatographic Fingerprint Evaluation System 2012' is adopted, and the fingerprint is formed by chromatographyinformation of 42 common peaks; through comparison with the retention time of medicinal materials of all compositions and the reference substance and an ultraviolet spectrogram, the common peaks attributed and part chromatographic peaks are identified, so that a technical means of quality control by a single ingredient or various effective ingredients is changed.

The invention is related to method for controlling quality of traditional Chinese medicinal materials and productions. Comparing fingerprints of high performance liquid chromatography HPLC for ten batches of ganoderma lucidum spore, oil of ganoderma lucidum spore determines mutual characters so as to obtain standard fingerprints. There are 15 mutual peaks in fingerprints. There are 4 peaks with its area exceeding 5% of total peak area. Features of the invention are: simple, stable, high accuracy, good reproducibility, easy of mastering. The invention discloses total new method for controlling quality of ganoderma lucidum spore, and oil of ganoderma lucidum spore, and distinguishing true or false products.

The invention discloses a detection method of a fingerprint spectrum of kidney-tonifying pregnancy-assisting granules. The detection method comprises the following steps: step 1, preparing a kidney-tonifying pregnancy-assisting granule test solution; step 2, preparing a mixed reference substance solution; step 3, precisely sucking the mixed reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and recording chromatograms; step 4, exporting a fingerprint spectrum instrument of the kidney-tonifying pregnancy-assisting granules, importing the fingerprint spectrum instrument into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of kidney-tonifying pregnancy-assisting granules as common peaks; generating a reference fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules by usingan average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing the fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules with the spectrum of a mixed standard substance, and identifying the peak of main components. The fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules provided bythe invention can comprehensively and objectively represent the quality of the kidney-tonifying pregnancy-assisting granules. And the detection method has the advantages of simplicity, convenience, stability, high precision, good reproducibility and the like.

The invention relates to a determination method for a fingerprint chromatogram of a HuangShiXiangShengWan preparation and a standard fingerprint chromatogram thereof. The determination method comprises the steps of preparing a test article solution, preparing a reference product solution, injecting the test article solution and the reference product solution into a gas chromatograph to carry out determination, and on the basis of the standard fingerprint chromatogram, judging whether the quality of the obtained HuangShiXiangShengWan is qualified data by using the determination method. The determination method is simple, fast, accurate, stable and reliable, can be used for controlling the quality of a HuangShiXiangShengWan sample, can be used for evaluating the quality of the HuangShiXiangShengWan in a comprehensive, objective and scientific manner and can guarantee effectiveness of the preparation.

The invention relates to a determination method for the HPLC-ELSD fingerprint spectrum of Lotrimin Sol Lotrimin. The determination method comprises the following steps of: preparing a sample solution;preparing a control sample solution; injecting the solutions into a liquid chromatograph (evaporative light scattering detector) to determine; and judging whether the quality data of the Lotrimin SolLotrimin obtained by the determination method is qualified or not on the basis of the standard fingerprint spectrum. The method is simple, rapid, accurate, stable and reliable, can be used for the quality control of a Lotrimin Sol Lotrimin sample, and can comprehensively, objectively and scientifically evaluate the quality of the Lotrimin Sol Lotrimin so as to guarantee the effectiveness of the preparation.

The invention discloses a method for detecting fingerprints of Mingge Shuqing Drink, the method comprising the following steps: step 1, preparation of Mingge Shuqing drink test solution; step 2, preparation of a mixed reference solution; step 3, precise absorption of the mixed control The product solution and the test solution are injected into the liquid chromatograph, and the chromatograms are recorded; step 4, the Ming Ge Shu Qing drink fingerprint instrument obtained in step 3 is exported, and imported into the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints, and different batches of fingerprints are selected. The chromatographic peaks that all exist in the chromatogram of Geshu Qingyin are used as common peaks; the control fingerprint of Minggeshu Qingyin is generated by the average value calculation method; the fingerprint of Minggeshu Qingyin obtained in step 5 and step 3 is compared with the mixed standard spectrum , to identify the main components. The fingerprint of Minggeshu Qingyin provided by the invention can comprehensively and objectively characterize the quality of Minggeshuqingyin; and has the advantages of simple, stable, high precision, good reproducibility and the like.

The invention provides a method for detecting quality of medicinal water extracts of Qipi oral liquid based on an HPLC (High Performance Liquid Chromatography) fingerprint spectrum. The method comprises the following steps of: preparing a comparison product solution and a tested sample solution; performing HPLC measurement; establishing a standard fingerprint spectrum; and detecting the sample. According to the method, a freezing technology and quality of the medicinal water extracts of the Qipi oral liquid can be effectively represented, energy source is saved and labor hour is reduced; the method is beneficial to monitoring the quality of products; and the method has the advantages of simple method, excellent stability, high precision, excellent repeatability, and the like.

The invention relates to a method for constructing an HPLC fingerprint of Xiaoerfeike granules, which belongs to the field of medicine. According to the HPLC fingerprint of Xiaoerfeike granules, the invention optimizes the conditions of the mobile phase, detection wavelength, gradient elution procedure and the like, and establishes the detection conditions of the fingerprint. Based on multiple batches of samples, a standard fingerprint of Xiaoerfeike Granules was established, which identified 24 common peaks, and carried out the identification of medicinal materials and components of the related common peaks. The method of the invention can comprehensively control the quality of Xiaoer Feike Granules, so as to better ensure the quality stability, consistency and controllability of Xiaoer Feike Granules, thereby ensuring the safety and effectiveness of Xiaoer Feike Granules . The present invention comprehensively analyzes the status of each component in Xiaoer Feike Granules through systematic identification of components and the attribution of single medicinal materials with common peaks in different batches of samples, which can provide an important reference for the quality control of Xiaoer Feike Granules.