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50results about How to "Effectively Characterize Quality" patented technology

Construction method of wolfberry fruit polysaccharide multi-element fingerprint spectrum and wolfberry fruit polysaccharide standard fingerprint spectrum

The invention provides a construction method of a wolfberry fruit polysaccharide multi-element fingerprint spectrum and a wolfberry fruit polysaccharide standard fingerprint spectrum, belonging to the technical field of traditional Chinese medicine and functional food raw materials and a fingerprint spectrum of the product. The construction method comprises the following steps: pretreating wolfberry fruits, extracting wolfberry fruit polysaccharide, determining a UV standard fingerprint spectrum, determining an HPSEC standard fingerprint spectrum, analyzing an IR light spectrum fingerprint and determining the standard fingerprint spectrum, completely hydrolyzing, deriving a hydrolyzed product, and analyzing a PCD-HPLC fingerprint and determining the standard fingerprint spectrum. The pretreatment, extraction of the wolfberry fruit polysaccharide and construction method of the multi-element fingerprint spectrum of 16 types of wolfberry fruits in different production areas are the same as above and can be operated simultaneously and respectively. The construction method of the wolfberry fruit polysaccharide multi-element fingerprint spectrum has the advantages of simplicity in operation, stability, flexibility, high precision, good repeatability and the like; and the quality condition and production area resources of the wolfberry fruit polysaccharides can be grasped from a whole characteristic appearance of a chromatogram and a novel scientific method is provided for wolfberry fruit quality control and true and false identification.
Owner:CHINA PHARM UNIV

Method for detecting fingerprint spectrum of Guigui zhugan decoction

The invention discloses a method for detecting a fingerprint spectrum of Guigui zhugan decoction. The fingerprint spectrum is established by the following steps: S1, preparing a Guigui zhugan decoction test sample solution; S2, preparing a reference solution; S3, absorbing the test sample solution and the reference solution precisely and respectively and injecting the solutions into a liquid chromatograph, and recording a chromatogram map; and S4, outputting a Guigui zhugan decoction fingerprint spectrum obtained in the S3 into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks exiting in all chromatogram maps of Guigui zhugan decoction with different batches as common peaks and generating a contrast fingerprint spectrumof the Guigui zhugan decoction based on an average value calculation method, and calculating relative retention time and relative peak areas of all common peaks. According to the invention, the detecting method having advantages of high stability and precision, and high reproducibility and the like is capable of analyzing the effective components in the Guigui zhugan decoction accurately and hasthe important significance in evaluating the quality of the Guigui zhugan decoction comprehensively and objectively.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +2

Shenqi hypoglycemic preparation HPLC standard finger print and construction method thereof

The present invention discloses a Shenqi hypoglycemic preparation HPLC standard finger print and a construction method thereof. The construction method comprises the following steps: preparing test sample solutions and a reference solution, measuring the test sample solutions and the reference solution by using the HPLC method and the linear gradient elution process, so as to obtain the Shenqi hypoglycemic preparation finger prints, wherein the chromatographic condition is that a mobile phase A is acetonitrile, a mobile phase B is 0.1% (v / v) phosphoric acid solution, the detection wavelength is 203nm, the column temperature is 25-35 DEG C and the flow rate is 0.8-1.2mL / min. The common characteristic peaks of at least ten finger prints are used as the characteristic peaks of the standard finger print; the standard finger print comprises 38 characteristic peaks, and the numbers of the characteristic peaks of saponin of ginseng stem and leaf, astragalus, Chinese magnoliaving, rehmannia root and raspberry are 13, 4, 7, 3 and 4 respectively. The Shenqi hypoglycemic preparation HPLC standard finger print and the construction method thereof can comprehensively and accurately evaluate the whole quality of the Shenqi hypoglycemic preparation, and facilitate to ensure the quality and the clinical curative effect of the Shenqi hypoglycemic preparation.
Owner:广东万年青制药股份有限公司

Method for detecting amino acid component in Xiasangju preparation

The related detection method for amino acid content in Xiasangju preparation comprises: (a) preparing reference solution; (b) preparing sample solution; (c) chromatogram condition: using octadecylsilicane chemically bonded silica as filler, using the gradient elution liquid with 0.05~0.10mol / L NaAc solution buffer and 20-80% acetonitrile solution as mobile phase with pH value as 4.50-5.05, 30-50Deg temperature, activation wavelength 250nm, emission wavelength 395nm, flow rate as 0.5-1.5mL .min-1, and 30-80min; (d) obtaining the fingerprint spectrum with HPLC. This invention is simple and stable, and has well repeatability and precision.
Owner:GUANGZHOU XINGQUN PHARMA

Establishment of Xiasangju preparations fingerprint pattern and fingerprint pattern thereof

This invention relate to the found of summer mulberry chrysanthemum preparation finger print, refer methodical control of quality of Chinese traditional medicine preparation. The invention is a method of founding HPLC finger print to detect alcohol extracted thing in summer mulberry chrysanthemum preparation. The method include;(a) preparation of control article solution;(b) preparation of examining solution; (c) chromatographic condition; chromatographic column take octadecyl silane bonded silica gel as padding;adopt gradient elution, mobile phase is gradient elution liquid composed by 0.1 to 3 percent acetic acid and methanol; column temperature;25 to 50 deg; ultraviolet detecting wavelength is 285 to 295 nm;velocity of flow;0.5 to 1.5 ml per min; time;30 to 80 min;(d) measuring; high efficiency liquid chromatography to gain finger print. This invention can validly characterize quality of summer mulberry chrysanthemum preparation, in favor of monitoring product quality; possess merit of method handiness, stable, high degree of precision, good reappearance; the invention can quickly and exactly discriminate the product.
Owner:GUANGZHOU XINGQUN PHARMA

HPLC fingerprint spectrum determination method for cannabis sativa

The invention provides an HPLC fingerprint spectrum determination method for cannabis sativa. The method is characterized by comprising the steps of (1) preparing a test solution: taking the cannabissativa medicinal material, crushing, sieving and baking the cannabis sativa medicinal material, precisely weighing baked cannabis sativa medicinal material powder, adding an extracting agent, carryingout ultrasonic extraction, carrying out standing until the room temperature is reached, passing through a microporous filter membrane, and taking subsequent filtrate; (2) preparing a reference solution: taking cannabidiolic acid, cannabidivarin, cannabigerol, cannabidiol, delta 9-tetrahydrocannabinol, cannabichromene, tetrahydrocannabinol and geranylflavone A reference substances, and adding methanol to dissolve the reference substances so as to prepare the reference substance solution; and (3) performing determination: precisely sucking the reference solution and the test solution into an HPLC, performing determination, and recording a chromatogram.
Owner:HANYI BIO TECH CO LTD

Establishment of lucid ganderma spore and lucid ganderma spore oil finger print atlas and standard finger print atlas

The invention is related to method for controlling quality of traditional Chinese medicinal materials and productions. Comparing fingerprints of high performance liquid chromatography HPLC for ten batches of ganoderma lucidum spore, oil of ganoderma lucidum spore determines mutual characters so as to obtain standard fingerprints. There are 15 mutual peaks in fingerprints. There are 4 peaks with its area exceeding 5% of total peak area. Features of the invention are: simple, stable, high accuracy, good reproducibility, easy of mastering. The invention discloses total new method for controlling quality of ganoderma lucidum spore, and oil of ganoderma lucidum spore, and distinguishing true or false products.
Owner:GUANGZHOU HANFANG PHARMA

High performance liquid chromatography (HPLC) standard fingerprint spectrum of lucid ganoderma capsule preparation and construction method and application of standard fingerprint spectrum

The invention discloses a high performance liquid chromatography (HPLC) standard fingerprint spectrum of a lucid ganoderma capsule preparation and a construction method and application of the standard fingerprint spectrum. The construction method comprises the following steps: preparing a test solution and a reference substance solution, and carrying out linear gradient elution, wherein a mobile phase A is acetonitrile, a mobile phase B is 0.05-0.15 percent (v / v) phosphoric acid aqueous solution, the detection wavelength is 252nm, the column temperature is 25-35 DEG C, the flow velocity is 1.0mL / min; measuring through HPLC, obtaining a lucid ganoderma capsule fingerprint spectrum, and taking a common characteristic peak of at least 14 batches of fingerprint spectrums as the characteristic peak of the standard fingerprint spectrum, wherein the standard fingerprint spectrum totally has 40 characteristic peaks, and the numbers of characteristic peaks belonging to lucid ganoderma, bighead atractylodes rhizome, rhizoma polygonati, the seed of Chinese dodder, schisandra chinensis, liquorice and the fruit of Chinese wolfberry are respectively 20, 3, 3, 5, 1, 5 and 2. According to the method and the standard fingerprint spectrum, the overall quality of the lucid ganoderma preparation can be comprehensively and accurately evaluated, and the quality and drug effect of the lucid ganoderma preparation are guaranteed.
Owner:INFINITUS (CHINA) CO LTD

Method for evaluating quality of loquat leaf medicinal materials

The invention relates to a method for building a fingerprint of loquat leaf medicinal materials and a fingerprint thereof. The method comprises the following steps of: firstly collecting the loquat leaf medicinal materials for over 10 batches, preparing test sample solution and a reference product solution, implementing measurement by referring to and applying a high-efficiency liquid chromatography method and a high-efficiency capillary electrophoresis method, and finally implementing analysis by applying a computation software of the similarity between the fingerprint of traditional Chinese medicines (which is recommended by the State Pharmacopoeia Committee). The method of the invention has the advantages of simplicity, stability, high precision, good reproducibility and easy grasp, can grasp the variety and the quality condition of the loquat leaves from the integral characteristic face and the high profile of chromatogram, and can be used as one of the methods for evaluating the quality of the loquat leaves and distinguishing the loquat leaves between true and false.
Owner:PUTIAN INST OF TRADITIONAL CHINESE MEDICINE

Establishment method of compound rheum officinale Qingyi decoction fingerprint

The invention provides an establishment method of a compound rheum officinale Qingyi decoction fingerprint. The establishment method comprises the following steps: preparation of a sample solution fortest, a single-flavor ingredient medicinal material sample solution for test and a reference substance solution, determination of chromatographic conditions of an HPLC (high performance liquid chromatography), and determination of a fingerprint, wherein the conditions of the HPLC are described as follows: a column is UItimate LP-C18 (250mm*4.6mm, 5mum), the flow velocity is 1.0mL / min, the columntemperature is 30 DEG C, the detection wavelength is 237nm, acetonitrile (A)-0.1% phosphoric acid solution (B) is adopted, and gradient elution is adopted. In the establishment method, the compound rheum officinale Qingyi decoction fingerprint established in 'Traditional Chinese Medicine Chromatographic Fingerprint Evaluation System 2012' is adopted, and the fingerprint is formed by chromatographyinformation of 42 common peaks; through comparison with the retention time of medicinal materials of all compositions and the reference substance and an ultraviolet spectrogram, the common peaks attributed and part chromatographic peaks are identified, so that a technical means of quality control by a single ingredient or various effective ingredients is changed.
Owner:ZUNYI MEDICAL UNIVERSITY

Glossy privet fruit fingerprint spectrum detection method

The invention discloses a glossy privet fruit fingerprint spectrum detection method. Building of glossy privet fruit fingerprint spectrums includes the following steps that 1, a test solution is prepared; 2, a mixed comparison product solution is prepared; 3, the test solution are accurately sucked to be injected into a liquid chromatograph, and chromatogram maps are recorded; and 4, the glossy privet fruit fingerprint spectrums obtained in the step 3 are subjected to instrument guiding-out, a traditional Chinese medicine chromatography fingerprint spectrum similarity evaluation system is guided in, chromatographic peaks existing in the chromatography maps of glossy privet fruits in different batches are selected as common peaks; comparison fingerprint spectrums of the glossy privet fruitsare generated by an average calculation method; and the relative retention time and the relative peak area of all common peaks are calculated. By means of glossy privet fruit fingerprint spectrums, the quality of the glossy privet fruits can be comprehensively and objectively represented. The fingerprint spectrum detection method has the advantages of the simple method, stability, high precision,good reproducibility and the like.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1

Establishment of Fingerprints of Herbal Jelly and Its Fingerprints

The invention discloses an establishment of a mesona blume medicinal material finger print, and a finger print thereof, and relates to a quality control method for traditional Chinese medicinal materials, specifically relates to a method for detecting an extracting solution of the mesona blume medicinal material through establishing HPLC finger print. The method comprises the following steps: preparing a reference substance solution; preparing a tested sample solution; adopting octadecylsilane chemically bonded silica gel as a filling for a chromatographic column; adopting gradient elution, wherein a mobile phase is a gradient eluent comprising 1.0-3.0% of formic acid and acetonitrile, a column temperature is 20-40 DEG C, an ultraviolet detection wavelength is 300-360 nm, a detection time is 90-120 minutes; carrying out high performance liquid chromatography to detect the finger print. According to the present invention, the quality of the mesona blume medicinal material can be effectively characterized so as to monitor the quality of the medicinal material; the method has characteristics of simplicity, stability, high precision, good reproducibility, and the authenticity of the medicinal material can be quickly and accurately identified.
Owner:DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE

Establishment of lucid ganderma spore and lucid ganderma spore oil finger print atlas and standard finger print atlas

The invention is related to method for controlling quality of traditional Chinese medicinal materials and productions. Comparing fingerprints of high performance liquid chromatography HPLC for ten batches of ganoderma lucidum spore, oil of ganoderma lucidum spore determines mutual characters so as to obtain standard fingerprints. There are 15 mutual peaks in fingerprints. There are 4 peaks with its area exceeding 5% of total peak area. Features of the invention are: simple, stable, high accuracy, good reproducibility, easy of mastering. The invention discloses total new method for controlling quality of ganoderma lucidum spore, and oil of ganoderma lucidum spore, and distinguishing true or false products.
Owner:GUANGZHOU HANFANG PHARMA

Construction method and quality detection method of UPLC feature map of Hangzhou chrysanthemum medicinal material

The invention relates to a construction method and a quality detection method of a UPLC feature map of a Hangzhou chrysanthemum medicinal material. The construction method comprises the following steps: sample solution preparation; taking the Hangzhou chrysanthemum medicinal material, crushing, adding a solvent for extraction and filtering, wherein a filtrate is taken as a sample solution; reference solution preparation: taking chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, galuteolin and linarin and adding a solvent for dissolution, wherein an obtained solution isa reference solution; ultra performance liquid chromatography detection: absorbing the sample solution and the reference solution, injecting into a liquid chromatograph and detecting to obtain the UPLC feature map of the Hangzhou chrysanthemum medicinal material. The UPLC feature map is rich in characteristic peak information, can fully reflect the chemical composition of the Hangzhou chrysanthemum medicinal material and can also effectively characterize the qualities of a Hangzhou chrysanthemum decoction piece and standard decoction and identify the product authenticity.
Owner:GUANGDONG YIFANG PHARMA

Detection method for fingerprint of radix puerariae assorted Chinese herbal tea

The invention discloses a detection method for the fingerprint of a radix puerariae assorted Chinese herbal tea. The detection method comprises the following steps: 1, preparing a radix puerariae assorted Chinese herbal tea test solution; 2, preparing a mixed reference substance solution; 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording chromatogram maps; and 4, exporting the radix puerariae assorted Chinese herbal tea fingerprint, importing the fingerprint into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks existing in chromatograms of different batches of radix puerariae assorted Chinese herbal tea as common peaks, generating a contrast fingerprint spectrum of the radix puerariae assorted Chinese herbal tea by using an average value calculation method, calculating the relative retention time and the relative peak area of each common peak, comparing the fingerprint of the radix puerariae assorted Chinese herbal tea with the spectrum of the mixed standard substance, and identifying main component peaks. The radix puerariae assorted Chinese herbal tea fingerprint provided by the invention can comprehensively and objectively characterize the quality of the radix puerariae assorted Chinese herbal tea. And the detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1

Detection method for fingerprint spectrum of kidney-tonifying pregnancy-assisting granules

The invention discloses a detection method of a fingerprint spectrum of kidney-tonifying pregnancy-assisting granules. The detection method comprises the following steps: step 1, preparing a kidney-tonifying pregnancy-assisting granule test solution; step 2, preparing a mixed reference substance solution; step 3, precisely sucking the mixed reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and recording chromatograms; step 4, exporting a fingerprint spectrum instrument of the kidney-tonifying pregnancy-assisting granules, importing the fingerprint spectrum instrument into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of kidney-tonifying pregnancy-assisting granules as common peaks; generating a reference fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules by usingan average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing the fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules with the spectrum of a mixed standard substance, and identifying the peak of main components. The fingerprint spectrum of the kidney-tonifying pregnancy-assisting granules provided bythe invention can comprehensively and objectively represent the quality of the kidney-tonifying pregnancy-assisting granules. And the detection method has the advantages of simplicity, convenience, stability, high precision, good reproducibility and the like.
Owner:JIANGSU PROVINCIAL HOSPITAL OF TCM

Finger-print quality determination method on wuji powder preparation and intermediate thereof

The invention discloses a finger-print quality determination method on wuji powder preparation and intermediate thereof. The finger-print quality determination method includes the following steps: quantitative wuji powder preparation or intermediate thereof is weighed, at least one of solvents of water, methyl alcohol or ethyl alcohol is used for extraction, constant volume is carried out, so as to obtain test solution; the test solution is sucked and injected into a liquid chromatograph, and high efficiency liquid chromatography and gradient elution are adopted for carrying out determination, so as to obtain the finger-print of wuji powder preparation or intermediate thereof; wherein the chromatographic conditions of the high efficiency liquid chromatography include that the filler of chromatographic column is octadecylsilane chemically bonded silica, mobile phase A is methyl alcohol, mobile phase B is phosphoric acid aqueous solution, the volume ratio of the mobile phase A and the mobile phase B is gradually increased along with time, and detection wavelength is 285-305nm. The invention has the characteristics that method is simple and stable, has high precision and good reproducibility and is easy to grasp.
Owner:JIUZHITANG

Five magic skin healthy ointment fingerprint detection method

The invention discloses a five magic skin healthy ointment fingerprint detection method which includes the steps: 1 preparing five magic skin healthy ointment test solution; 2 preparing reference solution; 3 precisely sucking quantitative test solution and reference solution, injecting the solution into a liquid chromatograph and recording a chromatogram; 4 leading out a five magic skin healthy ointment fingerprint instrument obtained in the step 3, leading the chromatogram into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks of chromatograms of five magic skin healthy ointments in different batches as common peaks, generating the reference fingerprint of the five magic skin healthy ointments by an average computing method, and calculating relative retention time and relative peak area of the common peaks. The researched five magic skin healthy ointment fingerprint detection method has the advantages that the method is simple, clear in fingerprint, high in repeatability and precision and the like. The quality of the five magic skin healthy ointments can be comprehensively and objectively represented.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1

Detection method of fingerprint spectrum of double-flower sangju drink

The invention discloses a detection method of a fingerprint spectrum of a double-flower sangju drink. The detection method comprises the following steps: step 1, preparing a test solution of the double-flower sangju drink; step 2 preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording a chromatogram map; step 4, exporting the fingerprint spectrum instrument of the double-flower sangju drink in the step 3, importing the fingerprint spectrum instrument into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of double-flower sangju drink as common peaks; generating a contrast fingerprint spectrum of the double-flower sangju drink by using an average value calculation method; and step 5, comparing the fingerprint spectrum of the double-flower sangju drink with the spectrum of the mixed standard substance, and identifying main components. The fingerprint spectrum of the double-flower sangju drink can comprehensively and objectively characterize the quality of the double-flower sangju drink. The fingerprint detection method provided by the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Owner:南京南医堂健康产业发展有限公司

Establishment of mesona blume medicinal material finger print, and finger print thereof

The invention discloses an establishment of a mesona blume medicinal material finger print, and a finger print thereof, and relates to a quality control method for traditional Chinese medicinal materials, specifically relates to a method for detecting an extracting solution of the mesona blume medicinal material through establishing HPLC finger print. The method comprises the following steps: preparing a reference substance solution; preparing a tested sample solution; adopting octadecylsilane chemically bonded silica gel as a filling for a chromatographic column; adopting gradient elution, wherein a mobile phase is a gradient eluent comprising 1.0-3.0% of formic acid and acetonitrile, a column temperature is 20-40 DEG C, an ultraviolet detection wavelength is 300-360 nm, a detection time is 90-120 minutes; carrying out high performance liquid chromatography to detect the finger print. According to the present invention, the quality of the mesona blume medicinal material can be effectively characterized so as to monitor the quality of the medicinal material; the method has characteristics of simplicity, stability, high precision, good reproducibility, and the authenticity of the medicinal material can be quickly and accurately identified.
Owner:DONGGUAN MATHEMATICAL ENG ACAD OF CHINESE MEDICINE GUANGZHOU UNIV OF CHINESE MEDICINE

Detection method for fingerprint spectrum of instant spina date seed heart-calming drink solid beverage

The invention discloses a detection method for the fingerprint spectrum of an instant spina date seed heart calming solid beverage. The method comprises the following steps of: step 1, preparing a test solution; step 2, preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording chromatograms; step 4, exporting the fingerprint spectrum of the test solution from an instrument, importing the fingerprint spectrum into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of instant spina date seed heart-calming drink solid beverages as common peaks; generating a control fingerprint spectrum of the instant spina date heart-calming drink solid beverages by using an average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing with the spectrum with the spectrum of the mixed standard product, and identifying main component peaks. The fingerprint spectrum can comprehensively and objectively characterize the quality of the instant spina date seed heart-calming drink solid beverage. The detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1

Detection method for fingerprint spectrum of instant momordica grosvenori solid beverage

The invention discloses a method for detecting a fingerprint spectrum of an instant momordica grosvenori solid beverage. The method comprises the following steps: step 1, preparing a test solution; step 2, preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording a chromatogram map; step 4, exporting an instant momordica grosvenori solid beverage fingerprint spectrum instrument, importing into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of instant momordica grosvenori solid beverages as common peaks; generating a control fingerprint spectrum of the instant momordica grosvenori solid beverage by using an average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing by using a mixed standard product spectrum, and identifying main component peaks. The fingerprint spectrum established by the invention can comprehensively and objectively characterize the quality of the instant momordica grosvenori solid beverage. And the detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1

A kind of detection method of anti-lung cancer sea Mu recipe fingerprint

The invention discloses a method for detecting the fingerprint of anti-lung cancer sea mulberry recipe, comprising the following steps: step 1: preparation of a reference peak solution; step 2: preparation of a test solution of anti-lung cancer ha mu recipe; step 3: separately precise Inject the quantitative reference peak solution and the test solution into the liquid chromatograph, and record the chromatogram; Step 4: Export the Haimu Fang fingerprint instrument obtained in step 3, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system, select different The chromatographic peaks that exist in the chromatograms of the batches of Haimufang tested are regarded as common peaks, and the average value calculation method is used to generate the control fingerprint of Haimufang, and the relative retention time and relative peak area of ​​each common peak are calculated. The fingerprint spectrum of the Haimu prescription provided by the invention can comprehensively and objectively characterize the quality of the Haimu prescription, and is beneficial to comprehensively monitor the quality of the medicine. The fingerprint detection method provided by the invention has the advantages of simple method, stability, high precision, good reproducibility and the like.
Owner:QINGDAO MARINE BIOPHARMACEUTICAL RES INST +1

Detection Method of Fingerprint of Xiaoer Ganmaoshu Granules

The invention discloses a method for detecting the fingerprint of Xiaoer Ganmaoshu Granules. The method comprises the following steps: step 1, preparation of the test solution of Xiaoer Ganmaoshu Granules; step 2, preparation of a single-standard reference substance solution; step 3, precision Draw the test solution into the liquid chromatograph, and record the chromatogram; step 4, export the fingerprint instrument of Xiaoermaoshu granules obtained in step 3, and import it into the similarity evaluation system of Chinese medicine chromatographic fingerprints, and select different batches of Xiaoermaoshu The chromatographic peaks that exist in the chromatograms of Shu granules are taken as common peaks; the control fingerprint of Xiaoermaoshu granules is generated by the average value calculation method; the relative retention time and relative peak area of ​​each common peak are calculated. The fingerprint of Xiaoer Ganmaoshu granules provided by the present invention can comprehensively and objectively characterize the quality of Xiaoer Ganmaoshu granules. The fingerprint detection method provided by the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Owner:JIANGSU HONGDIAN RES INST OF TRADITIONAL CHINESE MEDICINE IND CO LTD +1

A kind of establishment method of dendrobium characteristic fingerprint

The invention relates to a method for establishing a characteristic fingerprint of Dendrobium, and the steps are as follows: 1. Preparation of standard sample solution: taking Dendrobium, pulverizing, sieving, adding methanol to ultrasonic, filtering, filtrate was concentrated to dryness by rotary evaporation, dissolved in methanol solvent, and finally determined (2) Preparation of reference substance solution: Precisely weigh a certain amount of isoschavtoside, dissolve and dilute it with methanol to prepare a reference substance solution; (3) Determination of standard fingerprint: put the standard sample solution into The solution and the reference solution were injected into the high-performance liquid chromatograph respectively, and the chromatographic peak of schaffertoside was used as the reference peak of the fingerprint to calculate the relative retention time and retention peak area of ​​each characteristic peak of the standard sample, and to formulate the standard fingerprint. In the method for constructing a fingerprint spectrum, the invention qualitatively characterizes the fingerprint characteristic peaks with a relative peak area greater than 5%, breaks through the excessive ambiguity of the previous fingerprint spectrum, further clarifies the main components of the fingerprint spectrum, and improves the integrity of the Dendrobium fingerprint spectrum. and ingredient specificity.
Owner:北京蓝标一成科技有限公司

Detection Method of Fingerprint of Lingguizhugan Decoction

The invention discloses a method for detecting fingerprints of Linggui Zhugan Decoction. The establishment of fingerprints includes the following steps: step 1, preparation of Linggui Zhugan Decoction test solution; step 2, preparation of reference solution; step 3, precise absorption respectively The test solution and the reference solution are injected into the liquid chromatograph, and the chromatogram is recorded; step 4, the Linggui Zhugan Decoction fingerprint instrument obtained in step 3 is exported, and imported into the Chinese medicine chromatographic fingerprint similarity evaluation system, and different batches are selected. The chromatographic peaks that all exist in the chromatograms of Linggui Zhugan Decoction of the second time are used as common peaks, and the control fingerprint of Linggui Zhugan Decoction is generated by the average value calculation method; the relative retention time and relative peak area of ​​each common peak are calculated. The detection method of the fingerprint of Linggui Zhugan Decoction provided by the present invention has the advantages of good stability, high precision, good reproducibility, etc., can accurately analyze the active ingredients in Linggui Zhugan Decoction, and can comprehensively and objectively evaluate Linggui Zhugan Decoction. quality is of great significance.
Owner:NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +2
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