The invention discloses a
high performance liquid chromatography (HPLC) standard
fingerprint spectrum of a lucid
ganoderma capsule preparation and a construction method and application of the standard
fingerprint spectrum. The construction method comprises the following steps: preparing a
test solution and a reference substance solution, and carrying out
linear gradient elution, wherein a mobile phase A is
acetonitrile, a mobile phase B is 0.05-0.15 percent (v / v)
phosphoric acid aqueous solution, the detection
wavelength is 252nm, the
column temperature is 25-35 DEG C, the flow velocity is 1.0mL / min; measuring through HPLC, obtaining a lucid
ganoderma capsule fingerprint spectrum, and taking a common characteristic peak of at least 14 batches of fingerprint spectrums as the characteristic peak of the standard fingerprint spectrum, wherein the standard fingerprint spectrum totally has 40 characteristic peaks, and the numbers of characteristic peaks belonging to lucid
ganoderma, bighead atractylodes
rhizome, rhizoma polygonati, the seed of Chinese dodder,
schisandra chinensis, liquorice and the fruit of Chinese wolfberry are respectively 20, 3, 3, 5, 1, 5 and 2. According to the method and the standard fingerprint spectrum, the overall quality of the lucid ganoderma preparation can be comprehensively and accurately evaluated, and the quality and
drug effect of the lucid ganoderma preparation are guaranteed.