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Detection Method of Fingerprint of Lingguizhugan Decoction

A fingerprint and detection method technology, which is applied in the detection field of traditional Chinese medicine prescriptions, can solve the problems of less research on detection methods, and achieve the effect of good reproducibility, high precision and good stability

Active Publication Date: 2021-04-06
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on the fingerprint detection methods of Lingguizhugan Decoction

Method used

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  • Detection Method of Fingerprint of Lingguizhugan Decoction
  • Detection Method of Fingerprint of Lingguizhugan Decoction
  • Detection Method of Fingerprint of Lingguizhugan Decoction

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1, a kind of detection method of Linggui Zhugan Decoction fingerprint, is characterized in that, comprises the following steps:

[0044] Step 1, the preparation of Linggui Zhugan Decoction test solution:

[0045] Precisely weigh 10 batches of Poria cocos, Guizhi, Atractylodes macrocephala and licorice, including 40g of Poria cocos, 30g of cinnamon twig, 20g of Atractylodes macrocephala and 20g of licorice. Add 1L of water for ultrasonic extraction, collect the medicinal solution by filtration, evaporate the medicinal solution to dryness, add 4 times the amount of 95% ethanol and heat under reflux for extraction for 3 hours, filter the supernatant, evaporate to dryness in a water bath, add 20mL methanol solution to dissolve, pass through 0.45μm Pore ​​filter membrane, get 10 batches of need testing solution;

[0046] Step 2, preparation of reference substance solution:

[0047] , the preparation method of the reference substance solution in step 2 is as follows: take acc...

Embodiment 2

[0064] Embodiment 2 methodological investigation

[0065] 1. Precision experiment

[0066] Precisely draw the reference substance solution under embodiment 1, and continuously inject samples 6 times by the chromatographic conditions under embodiment 1, the RSD of its main common peak retention time is all less than 0.95%, and the relative peak area RSD is all less than 2.44%, showing the precision of the instrument The degree is good. The specific experimental results are shown in Table 6 and Table 7.

[0067] Table 6 relative retention time

[0068] serial number peak 1 peak 2 peak 3 peak 4 peak 5 Peak 6 1 30.228 31.127 33.156 38.209 46.377 61.193 2 30.507 31.096 33.253 38.185 46.182 61.137 3 30.103 31.224 33.087 38.311 46.134 61.353 4 30.862 31.034 33.076 38.409 46.138 61.527 5 30.130 31.525 33.174 38.244 46.271 61.556 6 30.246 31.431 33.109 38.139 46.193 61.468 average value 30.346 31...

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Abstract

The invention discloses a method for detecting fingerprints of Linggui Zhugan Decoction. The establishment of fingerprints includes the following steps: step 1, preparation of Linggui Zhugan Decoction test solution; step 2, preparation of reference solution; step 3, precise absorption respectively The test solution and the reference solution are injected into the liquid chromatograph, and the chromatogram is recorded; step 4, the Linggui Zhugan Decoction fingerprint instrument obtained in step 3 is exported, and imported into the Chinese medicine chromatographic fingerprint similarity evaluation system, and different batches are selected. The chromatographic peaks that all exist in the chromatograms of Linggui Zhugan Decoction of the second time are used as common peaks, and the control fingerprint of Linggui Zhugan Decoction is generated by the average value calculation method; the relative retention time and relative peak area of ​​each common peak are calculated. The detection method of the fingerprint of Linggui Zhugan Decoction provided by the present invention has the advantages of good stability, high precision, good reproducibility, etc., can accurately analyze the active ingredients in Linggui Zhugan Decoction, and can comprehensively and objectively evaluate Linggui Zhugan Decoction. quality is of great significance.

Description

technical field [0001] The invention relates to a detection method of a traditional Chinese medicine prescription, in particular to a detection method of a fingerprint of Linggui Zhugan Decoction. Background technique [0002] Fingerprint refers to certain complex substances, such as traditional Chinese medicine, the DNA of a certain organism or a certain tissue or cell, and after the protein is properly processed, the chromatogram or spectrum that can mark its chemical characteristics is obtained by using certain analysis methods. . Traditional Chinese medicine fingerprint is a comprehensive and quantifiable means of identification. It is based on the systematic research on the chemical components of traditional Chinese medicine, and is mainly used to evaluate the authenticity, goodness and stability of the quality of Chinese medicinal materials and traditional Chinese medicine preparations. Traditional Chinese medicine and its preparations are multi-component complex sys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/06
Inventor 蔡宝昌陈海超李伟东金俊杰秦昆明李国维张金龙孙戡平
Owner NANJING HAICHANG CHINESE MEDICINE GRPCO LTD
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