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Bacillus cereus specificity color biochemical fast detection kit and detection method

A technology of Bacillus cereus and detection kits, applied in biochemical equipment and methods, methods based on microorganisms, measurement/inspection of microorganisms, etc., can solve the problem of high technical requirements, inability to distinguish live bacteria from dead bacteria Meet the detection needs and other issues, achieve a wide range of application prospects, save detection costs and time effects

Active Publication Date: 2008-07-09
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies also have certain defects, such as high technical requirements, professional technicians are required to operate, and the detection cost is expensive.
The PCR method needs to enrich bacteria first, and cannot distinguish live bacteria from dead bacteria, which is prone to false positives; immunological methods also need to prepare high-purity antibodies, so these technologies are difficult to meet the needs of enterprises and government regulators for the detection of a large number of food samples

Method used

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  • Bacillus cereus specificity color biochemical fast detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Chromogenic biochemical rapid detection kit for detection of Bacillus cereus in rice.

[0060] 1. Detection kit composition

[0061] (1) Basic components of chromogenic medium: peptone 8g, tryptone 4g, yeast powder 7g, phosphate 2.5g, sodium pyruvate 9g, magnesium chloride 0.8g, chromogenic base X-GLU 0.35g, agar 11g, Na 2 CO 3 1g, pH7.0±0.2;

[0062] (2) Medium supplement: Tween80;

[0063] (3) Antibiotic solution:

[0064] 10ml of polymyxin sulfate solution sterilized by membrane filtration: contains polymyxin B sulfate (100,000IU);

[0065] (4) Sample diluent: 0.85% sterilized physiological saline.

[0066] 2. Preparation of chromogenic medium:

[0067] Weigh 43.65g of the basic components of the chromogenic medium, dissolve it in 1000ml of distilled water, then add 800.1ml of Tween, oscillate, and mix well. Autoclave at 121°C for 15 minutes, cool to 40-50°C, add antibiotic solution, mix well, pour plate, and set aside.

[0068] 3. Artificial contamination o...

Embodiment 2

[0075] Chromogenic biochemical rapid detection kit for testing pork samples in the market.

[0076] 1. Kit composition:

[0077] (1) Basic components of chromogenic medium: peptone 10g, tryptone 5g, yeast powder 8g, phosphate 3g, sodium pyruvate 10g, magnesium chloride 1g, chromogenic base X-GLU 0.4g, agar 12g, Na 2 CO 3 1.5g, pH7.0±0.2;

[0078] (2) Medium supplement: Tween80;

[0079] (3) Antibiotic solution: 10ml of polymyxin sulfate solution sterilized by membrane filtration: contains polymyxin B sulfate (100,000IU);

[0080] (4) Sample diluent: 0.85% sterilized physiological saline.

[0081] 2. Preparation of chromogenic medium:

[0082] Weigh 50.9g of chromogenic medium dry powder, dissolve in 1000ml of distilled water, then add 0.2ml of Tween80, shake and mix well. Autoclave at 121°C for 15 minutes, cool to 40-50°C, add antibiotic solution, mix well, pour plate, and set aside.

[0083] 3. Sample testing process:

[0084] Aseptically weigh 25g of pork, shred it, ...

Embodiment 3

[0088] Chromogenic biochemical rapid detection method for the detection of cold salad (yuba) in supermarkets.

[0089] 1. Kit composition

[0090] (1) Basic components of chromogenic medium: peptone 12g, tryptone 6g, yeast powder 9g, phosphate 3.5g, sodium pyruvate 11g, magnesium chloride 1.2g, chromogenic base X-GLU 0.55g, agar 13g, Na 2 CO 3 2g, pH7.0±0.2;

[0091] (2) Medium supplement: Tween80;

[0092] (3) Antibiotic solution:

[0093] 10ml of polymyxin sulfate solution sterilized by membrane filtration: contains polymyxin B sulfate (100,000IU);

[0094] (4) Sample diluent: 0.85% sterilized physiological saline.

[0095] 2. Preparation of chromogenic medium:

[0096] Weigh 58.25g of chromogenic medium dry powder, dissolve it in 1000ml of distilled water, then add 0.3ml of Tween80, oscillate, mix well, adjust the pH to 7.0±0.2, autoclave at 121°C for 15min, cool to 40-50°C, add antibiotic solution , mix well and pour into a flat plate for later use.

[0097] 3. Sa...

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Abstract

The invention provides a coloring biochemical rapid detecting kit for bacillus cereus with specificity and a detecting method and relates to a method for detecting and identifying microbes through using specific enzyme of the microbes. The kit comprises basic components of chromogenic culture medium, adjunct enhancer of the culture medium, antibiotics solution and sample dilution. The method which uses the kit in coloring biochemical rapid detecting for bacillus cereus with specificity comprises following steps: using the components in the kit to prepare the chromogenic culture medium, coating the sample on the chromogenic culture medium according to required procedures, inspecting, and analyzing results. The coloring biochemical detecting method and the kit which are provided by the invention can detect and identify the bacillus cereus in the food and environment thoroughly, systematically and accurately with sensitive detection, short cycle and strong operability, are applied to handle samples with high flux, and can be widely applied in the fields of food hygiene, environmental monitoring and the like.

Description

technical field [0001] The invention relates to a detection method for microorganisms, in particular to a kit and a detection method for rapid detection and identification of microorganisms using specific enzymes of microorganisms. Background technique [0002] "Food is the most important thing for the people", food eats the material basis for human survival, and food safety is a major issue related to human health and social development. In recent years, vicious incidents of food safety at home and abroad have occurred continuously, especially foodborne diseases caused by Salmonella, enterohemorrhagic Escherichia coli, Listeria monocytogenes, Vibrio parahaemolyticus, and Bacillus cereus. According to statistics, about 1 / 3 of people in developed countries suffer from food-borne diseases every year, and there are about 76 million cases of food-borne diseases in the United States every year. Food-borne diseases have become a common concern at home and abroad. [0003] Bacillu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/085
Inventor 张淑红吴清平张菊梅郭伟鹏
Owner GUANGDONG INST OF MICROORGANISM
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