Quantitative detection method for multiple components of Qiju Dihuang Oral Liquid
A technique for rehmannia oral liquid and a detection method, which is applied in the detection field of traditional Chinese medicine, can solve problems such as the inability to comprehensively and objectively evaluate the quality of Qiju Dihuang oral liquid, and achieve the effects of simple method, avoiding one-sidedness, and good reproducibility
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Embodiment 1
[0046] Embodiment 1 A kind of detection method of Qiju Dihuang oral liquid multi-component quantification comprises the following steps:
[0047] Step 1, the preparation of Qiju Dihuang Oral Liquid need testing solution:
[0048] Take 10 mL of Qiju Dihuang oral liquid from the above three batches in Table 1, measure them accurately, place in an evaporating dish, evaporate to dryness in a water bath, add 5 ml of methanol for ultrasonic treatment (power 150W, frequency 20kHz) for 30 minutes, let cool, filter to obtain clear liquid, and set Make up to 10mL, pass through a 0.45μm microporous membrane to obtain the test solution.
[0049] Step 2, preparation of acid mixed reference substance solution:
[0050] Take an appropriate amount of chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, salvianolic acid B, and paeonol, weigh them accurately, and add methanol to make each 1 mL of green A mixed reference solution of 90u...
Embodiment 2
[0059] Embodiment 2 methodological investigation
[0060] 1. Precision research
[0061] The sample batch number prepared by the method of Example 1 is 140401 need testing solution, analyzed according to the detection method of Example 1, parallel injection 6 times, the injection volume is 10 μ L, with 5-HMF, morroniside, chlorogenic acid, RSD ( %) values were 1.32, 2.58, 3.45, 2.01, 1.94, 1.53, 1.49, 2.05, 2.53, 2.41, 0.98, 2.40, 2.58. The results showed that the parallel injection precision of the equipment was good.
[0062] 2. Stability research
[0063] The batch number of the sample prepared according to the method of Example 1 is 140401 test solution, analyzed according to the detection method of Example 1, using 0, 2, 6, 12, 18, and 24 hours for sample injection analysis, and the injection volume is 10 μL , with 5-HMF, morroniside, chlorogenic acid, cryptochlorogenic acid, loganin, paeoniflorin, verbascoside, luteolin, isochlorogenic acid B, isochlorogenic acid A,...
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