Detection method of fingerprint spectrum of double-flower sangju drink
A technology of fingerprints and detection methods, which is applied in the detection field of fingerprints of Shuanghua Sangju Decoction, can solve the problems of few quality detection methods of Shuanghua Sangju Decoction, etc., and achieve the possibility of reducing the possibility of manual processing, good reproducibility, avoid one-sided effects
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Embodiment 1
[0043] Embodiment 1 A kind of detection method of fingerprint of Shuanghua Sangju Drink comprises the following steps:
[0044] Step 1, the preparation of Shuanghua Sangju drink test solution:
[0045] Take 8 batches of Shuanghua Sangju Decoction samples (made from 30 parts of honeysuckle, 35 parts of mulberry leaves, 10 parts of chrysanthemum, 10 parts of mint, and 15 parts of cassia seed) 5g into a 100mL round bottom bottle, add 50mL of methanol solution, and reflux extraction 2h, filter, put the filtrate in a 100mL volumetric flask, add methanol to constant volume, and pass through a 0.45μm microporous membrane to obtain the test solution;
[0046] Step 2, preparation of mixed reference solution:
[0047] Take accurately weighed reference substances of chlorogenic acid, rutin, luteolin, cryptochlorogenic acid, and arurinine cassiatin, place them in a volumetric flask, set the volume to the mark with methanol, and shake well to prepare 0.0674mg / mL Chlorogenic acid, 0.0902 mg...
Embodiment 2
[0054] Legal research of embodiment 2 fingerprint spectrum detection method:
[0055] 1. Precision research
[0056] Get Shuanghua Sangju drink sample (30 parts of honeysuckle, 35 parts of mulberry leaves, 10 parts of chrysanthemum, 10 parts of peppermint, 15 parts of cassia seed are made), the sample number prepared by the method of embodiment 1 is S1 need testing solution, according to implementation Analysis of the detection method in Example 1, 6 parallel injections, the injection volume is 10 μL, with chlorogenic acid, rutin, luteolin, cryptochlorogenic acid, and orange cassiatin as reference peaks, by analyzing the HPLC fingerprint of the sample The peak area and retention time of the common peaks were calculated and the RSD value was calculated. The results are shown in Table 2. The results show that the parallel injection precision of the equipment is good.
[0057] Table 2 Precision Study Peak Area and Retention Time
[0058]
[0059] 2. Stability research
[00...
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