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Detection Method of Fingerprint of Ming Ge Shu Qing Drink

A technology of fingerprint spectrum and detection method, which is applied in the detection field of traditional Chinese medicine, can solve the problems of few quality detection methods of Mingge Shuqing Drink, and achieve the effects of simple method, good reproducibility and high precision.

Active Publication Date: 2022-02-18
陈斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few quality testing methods for Ming Ge Shu Qing Yin

Method used

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  • Detection Method of Fingerprint of Ming Ge Shu Qing Drink
  • Detection Method of Fingerprint of Ming Ge Shu Qing Drink
  • Detection Method of Fingerprint of Ming Ge Shu Qing Drink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 A method for detecting the fingerprint of Minggeshu Qingyin, comprising the following steps:

[0041] Step 1, preparation of Mingge Shuqing drink test solution:

[0042] Take 8 batches of Minggeshuqingyin traditional Chinese medicine samples (each batch is composed of: hawthorn 15g, cassia seed 10g, kudzu root 25g, chrysanthemum 15g, lotus leaf 10g, raw Pu'er 15g, wolfberry 10g) into a 1000mL round bottom bottle, add methanol solution 500mL, Soxhlet extraction (continuous reflux extraction method) at a temperature of 75°C for 2 hours, filtered, the filtrate was placed in a 1000mL volumetric flask, added methanol to constant volume, and passed through a 0.45μm microporous membrane to obtain the test solution;

[0043] Step 2, preparation of mixed reference solution:

[0044] Take accurately weighed reference substances of chlorogenic acid, cryptochlorogenic acid, puerarin, and aurantine cassiatin, place them in a volumetric flask, use methanol to set the vo...

Embodiment 2

[0050] Legal research of embodiment 2 fingerprint spectrum detection method:

[0051] 1. Precision research

[0052] According to the method of Example 1, Mingge Shuqing Drink (Chinese medicine composition is hawthorn 15g, cassia seed 10g, kudzu root 25g, chrysanthemum 15g, lotus leaf 10g, raw Pu'er 15g, wolfberry 10g) is prepared into numbering as S1 test solution, according to embodiment 1 The chromatographic conditions for detection and analysis, parallel injection 6 times, the injection volume is 10 μ L, with chlorogenic acid, cryptochlorogenic acid, puerarin, and orange cassiacin as reference peaks, by analyzing the peaks of the common peaks of the sample HPLC fingerprint The area and retention time were calculated and the RSD value was calculated. The results are shown in Table 1. The results show that the parallel injection precision of this equipment is good.

[0053] Table 1 Precision study peak area and retention time

[0054]

[0055] 2. Stability research

[...

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Abstract

The invention discloses a method for detecting fingerprints of Mingge Shuqing Drink, the method comprising the following steps: step 1, preparation of Mingge Shuqing drink test solution; step 2, preparation of a mixed reference solution; step 3, precise absorption of the mixed control The product solution and the test solution are injected into the liquid chromatograph, and the chromatograms are recorded; step 4, the Ming Ge Shu Qing drink fingerprint instrument obtained in step 3 is exported, and imported into the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints, and different batches of fingerprints are selected. The chromatographic peaks that all exist in the chromatogram of Geshu Qingyin are used as common peaks; the control fingerprint of Minggeshu Qingyin is generated by the average value calculation method; the fingerprint of Minggeshu Qingyin obtained in step 5 and step 3 is compared with the mixed standard spectrum , to identify the main components. The fingerprint of Minggeshu Qingyin provided by the invention can comprehensively and objectively characterize the quality of Minggeshuqingyin; and has the advantages of simple, stable, high precision, good reproducibility and the like.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine, in particular to a detection method of the fingerprint of Mingge Shuqing drink. Background technique [0002] Mingge Shu Qingyin is a kind of recuperating tea drink that contains the theory of traditional Chinese medicine developed by summarizing decades of clinical experience of Professor Xie Yingbiao, a famous Chinese medicine doctor in Nanjing, with the strong support of Nanjing Nanyitang. This product is mainly composed of hawthorn, cassia seed, kudzu root, chrysanthemum, lotus leaf, raw pu'er, wolfberry and other ingredients. It has the effects of lowering blood pressure, promoting dampness and reducing fat, and is beneficial for hypertensive patients to regulate the body. It is suitable for regulating blood pressure and dampness. Lipid-lowering, can effectively protect the cardiovascular and cerebrovascular, improve myocardial ischemia, and at the same time can lower blo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809G01N2030/884
Inventor 谢英彪侯俊于照峰陈斌
Owner 陈斌
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