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Detection method for fingerprint spectrum of instant momordica grosvenori solid beverage

A solid beverage and fingerprint technology, which is applied in the direction of measuring devices, material separation, and material analysis, can solve the problem of few quality detection methods for instant Luo Han Guo solid beverages, and achieve the effects of simple methods, good reproducibility, and avoiding one-sidedness

Pending Publication Date: 2022-05-13
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few quality detection methods for instant Luo Han Guo solid beverage

Method used

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  • Detection method for fingerprint spectrum of instant momordica grosvenori solid beverage
  • Detection method for fingerprint spectrum of instant momordica grosvenori solid beverage
  • Detection method for fingerprint spectrum of instant momordica grosvenori solid beverage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 A kind of detection method of fingerprint of instant Luo Han Guo solid beverage comprises the following steps:

[0047] Step 1, the preparation of instant Momordica grosvenori solid beverage test solution:

[0048] Get 8 batches of instant Luo Han Guo solid beverage clear cream samples 25g (3.25g of Luo Han Guo, 2.5g of ginseng, 2.5 parts of bellflower, 2 parts of mulberry leaves, 2 parts of chrysanthemum, 2 parts of reed root, 1.25 parts of light bamboo leaves, 1.25 parts of gardenia, Put 1.25 parts of dandelion, 1.25 parts of dried ginger, 1.75 parts of coix seed, 1.5 parts of orange peel, 1 part of licorice, and 1 part of hawthorn) into a 500mL round bottom bottle, add 300mL of methanol solution, ultrasonically extract for 30min, and centrifuge the extract at 12000 rpm for 5min , take the supernatant, and pass through a 0.45 μm microporous membrane to obtain the test solution;

[0049] Step 2, preparation of mixed reference solution:

[0050] Take accu...

Embodiment 2

[0058] Methodology research of embodiment 2 fingerprint spectrum detection

[0059] 1. Methodological investigation

[0060] 1.1 Inspection of precision

[0061] Get instant Luo Han Guo solid beverage sample 25g (composition and proportioning with the medicinal taste of embodiment 1), according to the preparation method of the test sample in the above-mentioned embodiment 1, prepare the test solution, continuous sampling 6 times, each injection volume 10 μ L , detect according to above-mentioned embodiment 1 chromatographic condition, measure its HPLC chromatogram, investigate 14 common fingerprint peaks in chromatogram, the result shows that the retention time RSD of common fingerprint peak is all less than 0.9%, and the common peak peak area RSD is all less than 1.6%, indicating that the precision of the instrument is better.

[0062] 1.2 Stability investigation

[0063] Get instant Momordica grosvenori solid beverage sample 25g (composition and proportioning with embodim...

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Abstract

The invention discloses a method for detecting a fingerprint spectrum of an instant momordica grosvenori solid beverage. The method comprises the following steps: step 1, preparing a test solution; step 2, preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording a chromatogram map; step 4, exporting an instant momordica grosvenori solid beverage fingerprint spectrum instrument, importing into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of instant momordica grosvenori solid beverages as common peaks; generating a control fingerprint spectrum of the instant momordica grosvenori solid beverage by using an average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing by using a mixed standard product spectrum, and identifying main component peaks. The fingerprint spectrum established by the invention can comprehensively and objectively characterize the quality of the instant momordica grosvenori solid beverage. And the detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.

Description

technical field [0001] The invention relates to a method for detecting the instant solid beverage of traditional Chinese medicine, in particular to a method for detecting the fingerprint of the instant Luo Han Guo solid beverage. Background technique [0002] With the development of industrialization, air pollution is more serious, which greatly threatens the human lungs. In addition, respiratory diseases also aggravate lung damage. Therefore, the key to preventing virus infection and clearing lung pollution is to regulate the function of the respiratory tract and lungs. This invention starts with clearing the lungs, clearing the phlegm, heat and stagnation of fire in the lungs, and also has the effect of nourishing yin and restoring yang, and at the same time enhances the immune function of the body , resist virus attack. [0003] Luo Han Guo, sweet and cool, is a good product for clearing lung heat, relieving cough and moistening dryness as the main medicinal material; c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/02G01N30/06G01N30/14G01N30/34G01N30/74
CPCG01N30/8686G01N30/02G01N30/06G01N30/14G01N30/34G01N30/8634G01N30/8679G01N30/74G01N2030/146
Inventor 李国维金俊杰刘爱国
Owner NANJING HAICHANG CHINESE MEDICINE GRPCO LTD
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