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Establishment method of compound rheum officinale Qingyi decoction fingerprint

A technique for fingerprinting and establishing methods, which is applied in the field of compound rhubarb Qingyi Decoction fingerprinting, can solve problems such as the internal quality of difficult compound preparations, and achieve the effects of accurate authenticity, high precision, and good reproducibility

Active Publication Date: 2018-03-30
ZUNYI MEDICAL UNIVERSITY
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Problems solved by technology

However, at present, only the "Chinese Pharmacopoeia" uses thin-layer chromatography and HPLC to identify the medicinal materials and detect the content of the main medicinal ingredients, so it is difficult to comprehensively evaluate the internal quality of the compound preparation

Method used

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  • Establishment method of compound rheum officinale Qingyi decoction fingerprint
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  • Establishment method of compound rheum officinale Qingyi decoction fingerprint

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Embodiment Construction

[0041] The embodiments of the present invention will be described in detail below in conjunction with the examples. If no specific conditions are indicated in the examples, it will be carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0042] The method for establishing the fingerprint of compound rhubarb clear pancreas soup comprises the following steps:

[0043] Step 1: Preparation of reference substance solution: respectively prepare geniposide, paeoniflorin, paeonol, aloe-emodin, rhein, emodin, chrysophanol, emodin methyl ether, honokiol, magnolol, woody hydrocarbon lactone, dehydrocorylidene, and tetrahydropalmatine reference substance stock solution; then take the above reference substance stock solution precisely, and add methanol solution to prepare each 1 mL containing geniposide, p...

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Abstract

The invention provides an establishment method of a compound rheum officinale Qingyi decoction fingerprint. The establishment method comprises the following steps: preparation of a sample solution fortest, a single-flavor ingredient medicinal material sample solution for test and a reference substance solution, determination of chromatographic conditions of an HPLC (high performance liquid chromatography), and determination of a fingerprint, wherein the conditions of the HPLC are described as follows: a column is UItimate LP-C18 (250mm*4.6mm, 5mum), the flow velocity is 1.0mL / min, the columntemperature is 30 DEG C, the detection wavelength is 237nm, acetonitrile (A)-0.1% phosphoric acid solution (B) is adopted, and gradient elution is adopted. In the establishment method, the compound rheum officinale Qingyi decoction fingerprint established in 'Traditional Chinese Medicine Chromatographic Fingerprint Evaluation System 2012' is adopted, and the fingerprint is formed by chromatographyinformation of 42 common peaks; through comparison with the retention time of medicinal materials of all compositions and the reference substance and an ultraviolet spectrogram, the common peaks attributed and part chromatographic peaks are identified, so that a technical means of quality control by a single ingredient or various effective ingredients is changed.

Description

technical field [0001] The invention relates to a method for establishing the fingerprint of compound rhubarb clear pancreas soup and the fingerprint spectrum of compound rhubarb clear pancreas soup established by the method. Background technique [0002] Traditional Chinese medicine fingerprints can more comprehensively reflect the information of chemical components in medicines, and are widely used in the quality control of Chinese medicinal materials or Chinese patent medicines. Traditional Chinese medicines and their preparations belong to multi-component complex systems, and the quality evaluation should adopt corresponding detection methods that can provide rich identification information. At the present stage, when most of the active ingredients of traditional Chinese medicine are not clear, the fingerprint of traditional Chinese medicine is of great significance for effectively controlling the quality of Chinese medicinal materials or Chinese patent medicines. Tradi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王森欧水平郑小翠王玉和周旭美
Owner ZUNYI MEDICAL UNIVERSITY
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