Bioreactor for three-dimensional cell perfusion culture

Abstract

The invention relates to a bioreactor for three-dimensional tissue cell perfusion culture. A reaction chamber, a liquid storage tank and a peristaltic pump are sequentially connected through transfusion pipes, wherein the reaction chamber comprises a box cover and a box body comprising lateral walls, a bottom wall, a cut-off device, an overflow insert plate, screen frames, a static pressure channel, a sluice channel and a draining channel; the cut-off device is a gate or a weir flow dam, and a plurality of uniformly arranged water holes are formed in the same plane along the longitudinal section of the cut-off device; an overflowing opening matched with the draining channel is formed in the lateral wall of the static pressure channel; and the screen frames are fixed on two lateral walls of the box body. The invention also relates to a bioreactor with a laminated structure, which can be used for solving the problem of cell death easily caused by non-uniform flow field and non-uniform nutritional transfer in the existing perfusion culture, is suitable for three-dimensional culture of tissue cells, can be used for improving the quality of tissue engineering products, and has the characteristics of good expansibility, strong universality and wide application range.

Description

technical field [0001] The invention belongs to the field of cell culture devices, and relates to a bioreactor suitable for perfusion culture of three-dimensional tissue cells, in particular to a bioreactor for co-culture of various tissues and organs. Background technique [0002] Usually, the engineered tissue (or three-dimensional structure) that has been constructed needs to be cultured in vitro for a period of time, so that it can be transplanted or used for research after it matures. The static culture method of changing the medium, that is, remove the old culture medium first, then add the new culture medium, and then put it in the incubator for static culture. Its advantage is that it is suitable for small batch culture, and the period of changing the medium can vary with the tissues and cells. and adopt corresponding strategies. But its disadvantages are obvious: first, the consumption of nutrient solution decreases progressively, while the metabolites increase con...

AI Technical Summary

Problems solved by technology

Using this kind of bioreactor, due to the surface tension of the culture medium, the pressure head caused by the inconstant and uneven flow rate flowing into the buffer tank is unstable, and the discharge slope of the high overflow dam cannot be absolutely flat, so the culture medium When the buffer tank accumulates potential energy and flows through the discharge slope of the high overflow dam, one or several uneven flow liquids are formed, and what's more, some areas of the discharge slope form a broken flow at a lower flow rate. As a result, an uneven flow field appears when the medium enters the flow field of the culture section, causing cells in some areas to receive little or no nutrients and metabolites cannot be eliminated in time, resulting in damage or death; and the existing Most of the screens are fixed on the bottom wall of the reaction chamber, and a certain bracket must be needed to fix the screen, which will cause a uniform flow field on the back side of the pillars of the fixed screen frame in the flow field direction. Some impact on some cultivation areas

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