Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of exogenous selenium-molybdenum composition in alleviating chromium stress in tobacco

A technology of exogenous selenium and composition, applied in the field of agriculture, can solve problems such as harming the health of smokers, unclear mechanism, and threat to the safety of cigarette products, so as to improve the antioxidant system, alleviate the inhibition of photosynthesis, and enhance the resistance to heavy metal stress effect of ability

Active Publication Date: 2022-07-26
HENAN AGRICULTURAL UNIVERSITY +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the heavy metal chromium will also pose a threat to the safety of cigarette products. Tobacco is easy to accumulate chromium, and the chromium in cigarette leaves can enter the human body with the mainstream smoke during smoking, thereby endangering the health of smokers.
[0004] In existing studies, the response mechanism of exogenous selenium and molybdenum alone to alleviate tobacco heavy metal stress has been studied, but the mechanism of exogenous selenium and molybdenum combination to relieve chromium stress in tobacco seedlings is still unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of exogenous selenium-molybdenum composition in alleviating chromium stress in tobacco
  • Application of exogenous selenium-molybdenum composition in alleviating chromium stress in tobacco
  • Application of exogenous selenium-molybdenum composition in alleviating chromium stress in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0033] Selection of raw materials: Tobacco variety Yunyan 87, which is widely grown in the country, was selected. Chromium is potassium dichromate with a concentration of 50 μmol / L.

[0034] Source of reagents: Sodium selenite, sodium molybdate, potassium dichromate solid powder were purchased from Zhengzhou Aikemu Chemical Co., Ltd., analytically pure reagents with a content of 99%.

[0035] Cultivation method: (1) Pretreatment of tobacco seeds: first disinfect with 10% sodium hypochlorite solution for ten minutes, then rinse with ultrapure deionized water, and soak the seeds for 8 hours; In the deionized water seedling tray of the grid sponge, 2-3 seeds per grid point, and then placed in an incubator at 28°C for germination in the dark; (3) About three weeks after the seeds germinate, use tweezers to germinate them. Tobacco seedlings with 2 cotyledons were transplanted into commercially available vermiculite boxes (the chemical composition of vermiculite is SiO 2 Al 2 O ...

Embodiment 1

[0050] The effect of the application of exogenous selenium and molybdenum composition on the biomass of tobacco seedlings under chromium stress, see for details figure 1 . The plant height, root length, leaf dry weight and root dry weight of the above five groups of tobacco seedlings were measured by the above-mentioned methods of agronomic traits and biomass determination and fresh weight of each part of tobacco samples.

[0051] Depend on figure 1 It can be seen that Cr stress significantly reduced plant height, root length, leaf dry weight and root dry weight, while the use of Se, Mo and Se+Mo all alleviated the inhibitory effect of Cr on the growth of tobacco seedlings. Compared with CK, the Cr treatment significantly decreased plant height and root length by 16.8% and 42.6%, and leaf dry weight and root dry weight of tobacco seedlings decreased by 28.0% and 38.9%, respectively. The addition of Se and Mo significantly promoted the growth of tobacco seedlings under chromi...

Embodiment 2

[0054] The effect of the application of exogenous selenium and molybdenum composition on the chlorophyll content in tobacco seedlings under Cr stress, see for details figure 2 . Using the above-mentioned physiological and biochemical assay methods, the chlorophyll content of the tobaccos of the above five groups was measured respectively.

[0055] Photosynthesis and other physiological processes are highly sensitive to heavy metals, and low concentrations of Cr can also inhibit photosynthesis in plants. like figure 2 As shown, chromium stress significantly reduced the chlorophyll content of tobacco seedlings by 41.0%, 30%, and 38%, respectively. Studies have shown that the addition of selenium and molybdenum alone can increase the content of chlorophyll under chromium stress. The addition of Se and Mo significantly increased the content of chlorophyll a by 26.7% and 36.2%, and the content of chlorophyll b by 34.7% and 101.1%, respectively. The content of T was significant...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of an exogenous selenium-molybdenum composition in relieving the chromium stress of tobacco seedlings, and belongs to the technical field of agriculture. Through indoor hydroponic experiments, the effects of selenium-molybdenum combination under Cr stress on biomass, chlorophyll content, antioxidant enzyme activity, rhizome ultrastructure, leaf tissue structure, and Cr subcellular distribution in tobacco seedlings were analyzed. The invention confirms that the combination of selenium and molybdenum can improve the growth status of tobacco under chromium stress, enhance the ability of tobacco to resist heavy metal stress, and provide an effective reference for exploring tobacco and for safe production of other crops.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to the application of an exogenous selenium-molybdenum composition in relieving the chromium stress of tobacco. Background technique [0002] Chromium (Cr) is a plant toxic metal. Excessive deposition of chromium in plant tissues can cause severe phytotoxicity such as reduced growth and biomass, chlorophyll degradation, delayed nutrient uptake, overproduction of reactive oxidative species (ROS), disturbance of antioxidant defense mechanisms and cellular ultrastructure Chromium toxicity also inhibits the function and regulation of various proteins in plants and causes chromosomal aberrations in plant tissues. [0003] Chromium is a heavy metal pollutant, which can directly affect the migration of free water in tobacco cells, thereby causing water loss and wilting of tobacco plants. Influence of tobacco quality. At the same time, heavy metal chromium will also pose a threat to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N59/00A01P21/00
CPCA01N59/00
Inventor 许嘉阳曲利利李翔郝浩浩韩丹贾玮许自成王迅唐培培汪雪雷
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products