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Sperm quality fast detection system and detection method based on micro-fluidic chip

A microfluidic chip and sperm quality technology, applied in the field of microfluidics, can solve the problems of lack of portability, non-portable application, single detection method, etc., and achieve the effects of easy on-site detection, low pollution, and low detection cost

Pending Publication Date: 2017-09-19
HEBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chip also needs to add a docking module, a negative pressure module, etc., and the application is not portable.
Application No. 201220566804.4 discloses a semen detection device, including a sample box, a sample card, a light source, a control circuit, a heating unit, and a microscope that can be connected to a camera device. This invention has a simple structure and low cost, but has a single detection method and needs to be connected to a microscope. Also not portable

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Embodiment Construction

[0020] Specific examples of the present invention are given below. The specific embodiments are only used to further describe the present invention in detail, and do not limit the protection scope of the claims of the present application.

[0021] The invention provides a rapid detection system for sperm quality based on a microfluidic chip (see Figure 1-4 , referred to as the system), including a microfluidic chip 1, a reader 2 and an intelligent terminal 3; Reader 2 is used to amplify the semen sample; the intelligent terminal 3 is any device (such as a smart phone, a tablet computer or a PC, etc.) with a camera function for collecting sperm images; the camera of the intelligent terminal Align with the lens barrel 23 of the reader 2;

[0022] The microfluidic chip 1 includes a microfluidic channel unit plate 17 and a substrate 18; the substrate 18 is a flat plate structure; the microfluidic channel unit plate 17 is arranged on the substrate 18, and the substrate 18 and th...

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Abstract

The invention discloses a sperm quality fast detection system and detection method based on a micro-fluidic chip. The system comprises the micro-fluidic chip, a reader and an intelligent terminal, wherein the micro-fluidic chip comprises a micro-fluidic channel unit plate and a substrate; the micro-fluidic channel unit plate is arranged on the substrate; the micro-fluidic channel unit plate comprises an inlet, a micro-fluidic channel, micro cavities and an air channel; one end of the micro-fluidic channel communicates with the inlet; the other end of the micro-fluidic communicates with n micro cavities arranged at equal distance; the air channel and the micro-fluidic channel are positioned on the same plane and are in adjacent arrangement; one end of the air channel is connected with a finger membrane pump; the other end of the air channel is sealed; an umbrella-shaped one-way micro valve is arranged on a trunk path of the air channel; an air overflow hole is formed in the umbrella-shaped one-way micro valve; the micro cavities are adjacent to the air channel; the reader comprises a first lens group, a second lens group, a lens barrel, a focusing screw and a light source; a camera of the intelligent terminal is aligned with the lens barrel of the reader.

Description

technical field [0001] The invention relates to the field of microfluidics, in particular to a microfluidic chip-based rapid sperm quality detection system and detection method. Background technique [0002] Sperm motility refers to the percentage of progressive motility sperm in semen. Since only sperm with progressive motility can have normal viability and fertilization ability, motility is closely related to female conception rate. Sperm survival rate (sperm survival rate) refers to the proportion of live sperm in semen, and the survival rate of sperm is evaluated by testing the integrity of sperm membrane. Eosin is commonly used in medicine for sperm survival test. In the test, the heads of live sperm are white and light pink, and the heads of dead sperm are red and dark pink. The lower limit of the reference value for sperm viability is 58%. Total number of sperm (total number of sperm) refers to the total number of sperm in a complete ejaculation, calculated by mult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/487B01L3/00
CPCG01N33/487B01L3/50273B01L2400/0481B01L2400/0487
Inventor 刘吉晓李博文周一笛杨若曦李铁军
Owner HEBEI UNIV OF TECH
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