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Detection kit for sperms and check method thereof

A detection kit and semen technology, which is applied in the field of medicine, can solve the problems of inability to comprehensively or easily detect multiple indicators of male sperm and single detection indicators, and achieve the effects of saving time, simple operation, and convenient use

Inactive Publication Date: 2011-02-02
上海淦峰生物技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the quality of male sperm involves many indicators such as pH value, semen volume, liquefaction time, and sperm density. The existing technologies or methods have single detection indicators or require specific equipment, which cannot be more comprehensive or easier to detect male sperm. indicators

Method used

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  • Detection kit for sperms and check method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A test kit for semen, which includes a measuring cup with a measuring scale, a cell staining plate, and a bottle of methylene blue with a concentration of 1.33×10 -5 mg / ml, pH test paper (pH6.1~10.0), a bottle of PBS and a dropper, wherein the cell staining plate includes a shell and a substrate, and there are control holes and more than one test hole in the center of the shell, and the substrate is There are water-absorbing material layer, filter membrane and standard color code. The water-absorbing material layer and filter membrane are located above the substrate and below the test hole of the shell; the absorbent paper is located below the filter membrane; the standard color code is located above the substrate and below the control hole. The color of the standard color code The color of the test result of adding cell staining solution to a sperm sample with a concentration of 20 million / ml.

Embodiment 2

[0027] A test kit for semen, which includes a condom with measuring scale, a cell staining plate, a bottle of toluidine blue (1.33×10 -5 mg / ml), pH test paper (pH6.4-8.0), a liquefaction cup with chymotrypsin dry powder attached to the inner wall, a bottle of PBS and two droppers, wherein the cell staining plate includes a shell, a substrate, and a control in the center of the shell hole and more than one test hole, the substrate has a water-absorbing material layer, a filter membrane and a standard color code, the water-absorbing material layer and the filter membrane are located above the substrate, and below the test hole of the shell; the water-absorbing paper is located below the filter membrane; the standard color code Located above the substrate and below the control well, the color of the standard color scale is the color of the test result of adding the cell staining solution to the sperm sample with a concentration of 20 million / ml.

Embodiment 3

[0029] 505 users were tested according to the following steps:

[0030] Precautions for semen collection and experimental preparation;

[0031] 1. Users need to abstain from sex for 3-7 days before the test (too long or too short abstinence time will affect the accuracy and repeatability of the results), and the following methods are optional:

[0032] 2. How to collect semen: couples having intercourse, use the special condom in the kit to collect; or masturbation method, collect all the semen in the sample cup, after the liquefaction test is completed or skip the liquefaction test, the liquefaction agent in the rapid liquefaction capsule can be Pour into the sample, stir the dropper gently and let it stand for 15 minutes. The test can only be started after the semen is completely liquefied;

[0033] 3. The test should be carried out within 2 hours after semen collection, and it is best not to leave it for more than 12 hours

[0034] Testing method;

[0035] 1. Observe the...

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Abstract

The invention relates to the technical field of medicaments, in particular to a detection kit for sperms and a check method thereof. The invention discloses the detection kit for the sperms and the check method thereof. Many indexes of the sperms such as pH value, sperm volume, liquefying time, sperm density and the like can be quickly and accurately detected by adopting a sample so as to comprehensively reflect the sperm quality; meanwhile, compared with the traditional method, the method saves much time and has simple operation and convenient use; and the detection kit is purchased in a pharmacy and used at home, and is convenient for a detected person to know many indexes of own sperms.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a semen detection kit and a detection method thereof. Background technique [0002] In general, almost all animals, from fish to humans, have shown a decline in fertility over the past few decades. This decline is attributed to the pollution of the world environment, global warming, this decline is real, but the reason is not clear. [0003] Regardless of strain, several techniques are available to test for fertility in female mates, including physical examination, hormone testing, incubation of eggs, and other tests, but there are only two methods for determining fertility in male mates: "sperm count" and chemical examine. As of now, most low-fertility males are judged by sperm counts. This makes it difficult to detect chemical defects in males without concomitant sperm count problems. To be determined in a "sperm count", some magnification is done to increase the apparent ...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N33/487G01N1/30
Inventor 施惠娟范代娣陆明兰袁瑶王健王会蔡玲琍
Owner 上海淦峰生物技术发展有限公司
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