34 results about "Dna staining" patented technology

This Invention, a kind of DNA-based integrated circuit, with the DNA dyeing technology, the inlaying principle between anti-cancer medicine and DNA molecules, can change energy gap of DNA molecules to alter the conductivity of DNA molecules. Because that the diameter of DNA molecules is only about 2 nm, this kind of electronic element, which is not made with photolithography technologies, not only avoids the bottleneck of line width in production of photolithography-based ICs, but also limits the line width to 2 nm, much less than the minimum line width (0.13 mum or 130 nm) in semi-conductor production industry. It brings a practical approach to IC design beyond photolithography technologies, and ensures the development of ICs to micro-miniature predicted by the Moore Law.

The present invention is cell DNA staining kit for use in life science and medicine, especially the sole staining of cell nucleus, and its preparation process. The kit includes DNA staining solution, DNA fixing solution and DNA rinsing liquid in the volume ratio of 1.05 to 2 to 1. It is prepared through the following steps: preparing DNA staining solution, preparing DNA fixing solution, preparing DNA rinsing liquid, preparing cell preserving solution, and setting slide glass, the DNA staining solution, the DNA fixing solution, the DNA rinsing liquid and the cell preserving solution into a box to constitute the cell DNA staining kit. The present invention facilitates cell DNA staining and raises the accuracy.

The invention provides a cell DNA damage detection kit and a detection method thereof, relating to the cell DNA damage detection technique. The cell DNA damage detection kit comprises low melting-point agarose, a cell lysis solution, a cell despiralization solution and a DNA staining agent. The detection method comprises the following steps of: evenly mixing the cell DNA damage sample suspension with the low melting-point agarose gel, spotting the mixed liquid into sample holes on a gel board; immersing the gel board into the cell lysis solution for lysis in a dark place; immersing the gel board after lysis into the cell despiralization solution for despiralization, performing electrophoresis after despiralization; and dropwise adding the DNA staining agent for staining after electrophoresis, and analyzing the detection result under a fluorescence microscope. According to the detection method, the board cover of a cell culture board with 96 holes is used as the gel board to substitute for a glass slide for performing single cell gel electrophoresis, and compared with the traditional process in which 2 or 3 layers of gel are laid on a sample glass slide for performing single cell gel electrophoresis, two steps of gel laying and spotting are combined into one step, so that the sample preparation process is simple and easy to operate, the gel can not fall off and the success rate is high.

The invention discloses a cell nucleus DNA (Deoxyribose Nucleic Acid) staining method. The cell nucleus DNA staining method is characterized by comprising the following steps: 1, fixation: sequentially placing sample slices into a BS fixing solution at the temperature of 30-40 DEG C to fix for 20-30 minutes, and rinsing; 2, hydrolyzation: completely fixing the sample slices, hydrolyzing in 5N hydrochloric acid at the temperature of 30-40 DEG C for 20-30 minutes, and rinsing; 3, staining: after the hydrolyzation is completed, staining in a Thionin staining solution at the temperature of 30-40 DEG C for 30-40 minutes, and rinsing; 4, after the staining is completed, dewatering by adopting fast gradient ethanol, and mounting. In conclusion, compared with other Feulgen staining methods, the cell nucleus DNA cell nucleus DNA has the obvious advantages of practicability, stability, efficiency, cost saving, repeatability, compatibility of automated equipment and the like and can meet the requirement of clinical DNA quantitative cytology on fast diagnosis.

The invention provides a fluorescence in situ hybridization detection method of IncRNA in esophageal squamous carcinoma tissues. The method is characterized by comprising the following steps of preparing frozen esophageal squamous carcinoma tissue slices; performing fixation, digestion and penetration; using IncRNA probes for detection; performing DNA dyeing and chip sealing; using confocal microscopy detection imaging or fluorescence microscope detection imaging. The fluorescence in situ hybridization detection method of IncRNA in the esophageal squamous carcinoma tissues provided by the invention has the advantages that the cost is low; the operation is simple; the time and the labor are saved. The method can be used for conveniently detecting the expression level of the IncRNA in the esophageal squamous carcinoma tissues; the positioning conditions of the IncRNA in sub cells in the esophageal squamous carcinoma tissues can be directly shown.

The invention discloses a gauging method of milk cell quantity, which comprises the following steps: (1) adding the color-developing agent of random commissioning ratio in the crude milk; displaying color of crude milk in the boiling-water in the strong acidic condition; preparing the color-developing agent based on DNA dyeing agent; (2) placing the crude milk with color in the colorimetric device; adapting D65 as standard light source to make the distance between sample and standard light source as 45 cm; obtaining the color information of sample through image intaking equipment; 3) counting the color information to gain the average value of corresponding color system component; 4) calculating through the algorithm formula.

Disclosed herein are multiplex methods for co-detecting a B cell marker, TP53 nucleic acid, and Chromosome 17 centromere DNA in a single sample. Samples stained for the B cell marker (e.g., CD79a protein), TP53 nucleic acid, and Chromosome 17 centromere DNA may allow for the identification of the subtype of chronic lymphocytic leukemia (CLL) with the 17p deletion. The methods feature staining the B cell marker (e.g., CD79a) a first distinct color, TP53 in a second distinct color, and chromosome 17 centromere DNA in a third distinct color. Further disclosed are nucleic acid probes specific for 19q12, INSR, ATM, DLEU2, TP53, and 13q12.

The invention provides a fluorescence in-situ hybridization detection method for lncRNA in lung cancer tissue. The method comprises the steps of preparation of a lung cancer frozen section, tissue fixation, digestion and permeation, probe hybridization detection of lncRNA, DNA staining, section sealing and fluorescence detection imaging. Expression conditions of lncRNA in the lung cancer tissue can be detected accurately and directly, and subcellular localization of lncRNA in the lung cancer tissue is shown in situ. The fluorescence in-situ hybridization detection method for lncRNA in the lung cancer tissue is simple to operate and easy to popularize and has important medical application value.

A sperm chromatin structure detection kit and application thereof are provided. The kit includes an A reagent and a B reagent, with the A reagent including HCl, Tween-20 and a phosphate buffer solution, and the B reagent including acridine orange and water. The kit is used in human sperm chromatin DNA staining to determine the chromatin DNA fragmentation level and to calculate the ratio of spermswith DNA fragmentation, and can analyze the ratio of immature sperms through the degree of combination between chromatin and protein.

The invention discloses a cell nucleus DNA staining method. The method comprises the following steps: preparing an AF stationary liquid, a hydrolysate, a rinsing liquid and an eosin staining liquid; preparing a cell DNA staining solution which is composed of a cell DNA staining solution A and a cell DNA staining solution B in a volume ratio of 1: 1; placing a pathological sample into the AF stationary liquid to be fixed; washing the sample with running water, and then placing the sample into a hydrolysate to hydrolyze; washing the sample with running water, and then placing the sample in the cell DNA staining solution for staining; washing the sample with running water, and then placing the sample in a rinsing liquid for rinsing; and carrying out dehydrating with ethanol step by step, placing the sample into an eosin staining solution, and carrying out decolorizing with absolute ethyl alcohol, airing, and sealing. According to the method, dyeing time is greatly shortened, the hydrolysate is composed of non-precursor chemicals and belongs to an environment-friendly reagent, compared with a traditional stationary liquid, the AF stationary liquid does not include acetic acid and lesssimulates the human body, and the cell DNA staining solution is simple to prepare, does not need to be heated and boiled, has the validity period longer than 18 months, is ready-to-use and is suitablefor commercial popularization.

The invention relates to a method for dyeing DNA in cesium chloride density gradient mixed liquor through GelGreen dye, and belongs to the field of molecular biological technologies. The method includes the steps that step1, an environmental sample is collected, and the environmental total DNA is extracted; step2, environmental total DNA-cesium chloride mixed liquor is prepared, and the GelGreen dye is added into the environmental total DNA-cesium chloride mixed liquor; step3, ultrahigh-speed centrifugation is performed on the mixed liquor with the GelGreen dye to obtain the density gradient mixed liquor; step4, layering is performed on the density gradient mixed liquor under irradiation of a fluorescence transmission instrument with the excitation wavelength of 488 nm, and the density gradient mixed liquor in corresponding layers is extracted through an injector; step5, bacterium 16S rRNA gene real-time fluorescence quantified PCR analysis is performed on the DNA in the density gradient mixed liquor in all the layers. By dyeing the DNA in the cesium chloride density gradient mixed liquor based on the GelGreen dye, the detection sensitivity of the environmental DNA in the cesium chloride density gradient mixed liquor obtained after ultrahigh-speed density gradient centrifugation is greatly improved.

The invention discloses a comfrey extract, a preparation method and application thereof. After many years of hard work, the inventor discovered for the first time that comfrey extract and comfrey alkaloids can act on DNA chromosome complement (DNA base repair), and comfrey extract can strongly promote the uptake of glucose by liver cancer cell HepG2, and Both it and comfrey alkaloids have the effect of inhibiting aldose reductase. It shows that it has great potential in lowering blood sugar, treating diabetes and preventing and treating diabetic neuropathy. The comfrey extract and alkaloids of the present invention have the characteristics of rapid onset of action, short course of treatment, small side effects and the like in the treatment of diabetes. Moreover, the preparation method of the medicine of the present invention is convenient for large-scale production, and has important practical significance for the development of new enteric-coated oral preparations for treating diabetes.

The invention relates to a pathological automatic DNA (deoxyribonucleic acid) staining system. The system comprises a staining platform, a bearing frame, a shifting device and a mechanical arm, wherein a row of liquid tanks are embedded into the staining platform; the bearing frame is vertically connected with the staining platform; the shifting device is connected with the bearing frame in parallel; the mechanical arm is connected with the moving device in a sliding manner, and a mounting device is connected to the mechanical arm; a plurality of staining frames are hung at the lower end of the mounting device; a vent hole is formed in the bearing frame; each device is controlled to work by a microcomputer system, and a control window of the microcomputer system is formed in the side surface of the staining platform; and an air exhaust system and a power-off protection system are arranged in the pathological automatic staining system. By utilizing the technical scheme provided by the invention, the automatic operation of multiple sets of staining methods is realized, the staining is simple and stable, and the number of sheets stained once can be selected according to requirements, thus being suitable for requirements of different users.

The invention relates to a method for dyeing DNA in cesium chloride density gradient mixed liquor through GelGreen dye, and belongs to the field of molecular biological technologies. The method includes the steps that step1, an environmental sample is collected, and the environmental total DNA is extracted; step2, environmental total DNA-cesium chloride mixed liquor is prepared, and the GelGreen dye is added into the environmental total DNA-cesium chloride mixed liquor; step3, ultrahigh-speed centrifugation is performed on the mixed liquor with the GelGreen dye to obtain the density gradient mixed liquor; step4, layering is performed on the density gradient mixed liquor under irradiation of a fluorescence transmission instrument with the excitation wavelength of 488 nm, and the density gradient mixed liquor in corresponding layers is extracted through an injector; step5, bacterium 16S rRNA gene real-time fluorescence quantified PCR analysis is performed on the DNA in the density gradient mixed liquor in all the layers. By dyeing the DNA in the cesium chloride density gradient mixed liquor based on the GelGreen dye, the detection sensitivity of the environmental DNA in the cesium chloride density gradient mixed liquor obtained after ultrahigh-speed density gradient centrifugation is greatly improved.

The invention discloses a DNA staining and eosin staining comparative analysis method and system.The comparative analysis method comprises the steps of placing a DNA staining slide in an optical scanner through a slide loader, generating a digital image through a controller, and transmitting the image to a server; segmenting the digital picture through a first artificial intelligence algorithm, and classifying cell nucleuses in the digital picture into epithelial cell nucleuses, neutrophil nucleuses and lymphocyte nucleuses; recognizing epithelial cell nucleuses of which the DNA indexes are greater than a set threshold value, and storing the positions of the epithelial cell nucleuses in the whole slide; carrying out DNA eosin staining on the slide again, scanning the slide again, and showing the cytoplasm of the cell nucleus through a staining agent; cutting the epithelial cell nucleus of which the DNA index is greater than a set threshold value from the whole picture; and performing redetermination verification on the cut epithelial cell nucleus through a second artificial intelligence algorithm in the server to determine whether the epithelial cell nucleus is the epithelial cell nucleus. According to the method, the analysis accuracy can be improved.

The purpose of the present invention is to provide Agrobacterium with improved gene targeting efficiency. According to the present invention, provided is agrobacterium which includes a T-DNA nuclear transport ability similar to a wild type, and for which the T-DNA chromosome insertion ability is lost or reduced when compared to a wild type.

The invention relates to the technical field of biology, in particular to a DNA ploidy staining solution as well as a preparation method and a staining method thereof, and the prepared DNA staining solution can solve the problems that the background is easy to co-stain, the staining time is relatively long and slices are easy to fade in flaking, so that a reliable histochemical staining technologyis provided for tumor cell nucleus DNA content image determination. Alcohol with the concentration of 95% is used for fixing cell nucleuses, the situation that in the dyeing hydrolysis process of unfixed cell nucleuses, DNA can form small fragments and dissociate out of the cell nucleuses, the DNA measurement accuracy is affected is avoided, aldehyde groups generated before hydrolysis can be sealed through fixation, and false positive products are reduced. Meanwhile, the BS stationary liquid can be used for changing the space structure of DNA molecules, so that the sensitivity to acid hydrolysis is influenced, and the situation that chromatin coloring is relatively light due to improper selection of the stationary liquid or too long tissue fixing time and relatively large difference in strength of dyeing reaction is avoided. Through gradient alcohol dehydration, the DNA staining result is clear, and the background is cleaner.

The invention discloses a cancerous cell detection method and system. The detection method comprises the following steps: constructing a model; constructing a DNA staining cell nucleus recognition model and an eosin staining cell nucleus recognition model; executing a cancerous cell recognition step; selecting suspected cancer cells by using the DNA staining cell nucleus recognition model, and correcting the suspected cancer cells by using the eosin staining cell nucleus recognition model. According to the cancerous cell detection method and system provided by the invention, the cancerous cell detection accuracy can be improved, and meanwhile, the cancerous cell detection efficiency can be improved.

The invention relates to a DNA fragment staining kit. The DNA fragment staining kit comprises a carrier groove, a sealing cover, a reagent storage bottle, a guide pipe, a detection sensor, an illumination light source, a control circuit and a peristaltic pump, wherein the carrier groove and the sealing cover form a closed cavity structure, the interior of the carrier groove is divided by a partition plate into at least one carrier cavity and at least one detection cavity, one carrier cavity and one detection cavity form a detection group, both the detection sensor and the illumination light source are installed in each detection cavity, the reagent storage bottle is embedded in the carrier cavity and communicated with the guide pipe by virtue of the peristaltic pump, the peristaltic pump is embedded into the carrier groove and electrically connected with a control circuit, the control circuit is embedded into the sealing cover, and the sealing cover corresponding to the control circuitis provided with an operation interface. By adopting the DNA fragment staining kit, a DNA staining reagent can be effectively carried and positioned, and the DNA staining observation operation efficiency and accuracy can be greatly improved.

The present invention relates to a DNA logic integrated circuit and its production method. It uses double-chain DNA as substrate of semiconductor device, utilizes the DNA staining technique and mosaic principle of anticancer medicine and DNA between them to change energy gap of DNA molecule and further change the conductivity of DNA molecule. The diameter of DNA molecule is about 2 nm, and its minimum wire width is 2 nm only, and is far less than minimum wire width 0.13 micrometer (130 nm) of existent semiconductor technique, so that it does not use etching technique to make integrated circuit, and can make integrated circuit more miniature.

The invention discloses a method for rapidly identifying hybrids of gray feathers of Chinese goose species in the field of goose species detection. S1: collecting biological samples of gray feather individuals to be detected; S2: extracting genomic DNA from the biological samples of the individuals to be detected; S3: Using the extracted genomic DNA as a template, the designed and synthesized PCR primer combination is used as primers for conventional PCR reaction; S4: Use 1% agarose gel electrophoresis to separate PCR products, use DL2000 as DNAmarker, and Goldview I as DNA staining S5: Observe the amplified product under ultraviolet light or gel imaging system, if there is an amplified band at about 300bp, it is judged that the detected individual is a heterozygous individual, otherwise, if there is no amplified band at about 300bp, Then it is judged that the detected individual is a homozygous gray feather individual. Quickly and accurately identify gray feather heterozygous individuals, so as to eliminate them from the reserved breeding geese. Only one generation of detection is needed to make the breeding group achieve homozygous gray feather phenotype, improve breeding efficiency, and increase the purity of the population. Identify the method provided.

The invention discloses a GSTM1 typing test method based on a quantitative PCR technique. The typing test method adopts GSTM1 gene-specific primers, SEQ1 and SEQ2, to amplify a GSTM1 gene segment, and meanwhile a GSTM1 gene-specific TAQman probe, SEQ3-Fam, is designed in an amplified region which is defined by the GSTM1 gene-specific primers; ALbumin-specific PCR primers, SEQ4 and SEQ5, are adopted to amplify an Albumin gene segment, and meanwhile an ALbumin gene-specific TAQman probe, SEQ6-Vic, is designed in an amplified region which is defined by the ALbumin gene-specific primers. The typing test method can effectively prevent PCR product pollution, avoid contamination by poisonous DNA colouring agents, greatly simplify test and result analyzing processes, and increase test throughput.

The invention relates to the technical field of DNA polyhedron dyeing and recombination, in particular to a graph dyeing and recombination method of an addressable DNA polyhedron topological structure, which can solve the problem of conflict between repeatability and addressability in the DNA polyhedron topological structure consisting of repeated components. The graph dyeing method of the addressable DNA polyhedron topological structure comprises the steps that 1, each edge of a DNA polyhedron is dyed through two colors, the number of the edges of the two colors is equal, the edge of each color corresponds to one addressable assembly, and the addressable assemblies comprise the first addressable assembly and the second addressable assembly; 2, a plurality of first addressable assemblies and / or a plurality of second addressable assemblies are / is utilized to synthesize a DNA staining chain, and the DNA staining chain corresponds to a serial number, wherein the plurality of DNA stainingchains are in one-to-one correspondence with a plurality of numbers; and 3, the plurality of DNA staining chains are optimized to obtain a standard DNA staining chain. The method is used for graph dyeing and recombination of the addressable DNA polyhedron topological structure.

This Invention, a kind of DNA-based integrated circuit, with the DNA dyeing technology, the inlaying principle between anti-cancer medicine and DNA molecules, can change energy gap of DNA molecules to alter the conductivity of DNA molecules. Because that the diameter of DNA molecules is only about 2 nm, this kind of electronic element, which is not made with photolithography technologies, not only avoids the bottleneck of line width in production of photolithography-based ICs, but also limits the line width to 2 nm, much less than the minimum line width (0.13 mum or 130 nm) in semi-conductor production industry. It brings a practical approach to IC design beyond photolithography technologies, and ensures the development of ICs to micro-miniature predicted by the Moore Law.