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Cell DNA staining kit and its prepn process

A deoxyribonucleic acid and dyeing reagent technology, applied in the field of kits, can solve problems such as inaccuracy and cumbersome dyeing, and achieve the effects of solving cumbersome and inaccurate, simplified preparation process and stable effect.

Inactive Publication Date: 2007-10-03
SOUTHEAST UNIV
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Problems solved by technology

[0003] Technical problem: The purpose of the present invention is to solve the cumbersome and inaccurate problems of staining in the diagnosis of cell DNA in the field of life science and human medicine, to achieve simple, accurate and standardized detection of the DNA in the nucleus of the exfoliated cells of the organism. The target of specific staining, providing a cell deoxyribonucleic acid staining kit and its preparation method

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  • Cell DNA staining kit and its prepn process
  • Cell DNA staining kit and its prepn process
  • Cell DNA staining kit and its prepn process

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preparation example Construction

[0019] The preparation steps of the DNA fixative solution are as follows: 320ml of methanol, 60ml of formaldehyde, 20ml of glacial acetic acid, and then put into a 500ml bottle for packaging.

[0020] The preparation steps of the DNA rinse solution are as follows: add 1 g of sodium metabisulfite, add 2 ml of 5 N hydrochloric acid, add distilled water to 200 ml, put it in a 300 ml bottle and seal it.

[0021] The preparation steps of the cell preservation solution are as follows: 50% ethanol is added with 1% dithiothreitol (2:1) to add a total of 10 ml (2:1) into a 20 ml bottle for packaging.

[0022] The preparation steps of the cell preservation solution are as follows: 50% ethanol is added with 1% dithiothreitol (2:1) to add a total of 10 ml (2:1) into a 20 ml bottle for packaging.

[0023] Working principle: The aldehyde group of the oxygen molecule hydrolyzed by the DNA in the biological cell in an acidic environment combines with the dye to form a colored chemical reactio...

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Abstract

The present invention is cell DNA staining kit for use in life science and medicine, especially the sole staining of cell nucleus, and its preparation process. The kit includes DNA staining solution, DNA fixing solution and DNA rinsing liquid in the volume ratio of 1.05 to 2 to 1. It is prepared through the following steps: preparing DNA staining solution, preparing DNA fixing solution, preparing DNA rinsing liquid, preparing cell preserving solution, and setting slide glass, the DNA staining solution, the DNA fixing solution, the DNA rinsing liquid and the cell preserving solution into a box to constitute the cell DNA staining kit. The present invention facilitates cell DNA staining and raises the accuracy.

Description

technical field [0001] The invention is a kit for biological cell DNA specific staining applied in the field of life science and human medicine, especially directly applied to the single staining of cell nuclei in biological cells. Background technique [0002] The current cell DNA staining uses Schiff's reagent as the main component. Because the Schiff's reagent itself is also acidic, it will also cause the hydrolysis of DNA, resulting in inaccurate results. The quality of Schiff's reagent can not guarantee that the staining results of biological specimens can be effectively repeated, resulting in DNA detection results that cannot correctly reflect objective facts, thus causing human cognition in the field of life sciences and human medicine. hindered. At present, no identical or similar products have been found after searching the database of the State Food and Drug Administration. Contents of the invention [0003] Technical problem: The purpose of the present inventi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30C12Q1/68
Inventor 龚文涛姚斌
Owner SOUTHEAST UNIV
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