64 results about "Somatic cell count" patented technology

The invention relates to methods for producing a fermentation product from a lignocellulose-containing material comprising i) pre-treating lignocellulose-containing material; ii) hydrolysing pre-treated lignocellulose-containing material; iii) fermenting using a fermenting organism; wherein fermentation is initiated and carried out at: a) a fermentation organism cell count in the range from 10-250×1010 cells per L fermentation medium; or b) a fermentation organism concentration in the range from 2-90 g dry weight fermenting organism per L fermentation medium.

The present invention provides methods for treating an animal having a high somatic cell count. The methods include administering compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 10.0 endotoxin units per milliliter.

An on-line somatic cell analyser and a method for measuring a somatic cell count (SCC) are provided. A flow cell is connected to a milking hose and admits a constant volume of sampled milk into the flow chamber. A probe with two electrodes is positioned in a zone of optimal sensing inside the flow chamber, and provides a modulated signal according to the number of sodium ions present in the sample. A control unit receives the modulated signal, generates an ion count, and compares same with a plurality of SCC thresholds for classifying the sample in a quality category. A set of parameters characterizing the respective quality category, as well as the presence of mastitis in the animal, are finally displayed.

The invention discloses application of a cordyceps strain fermentation product to reduce somatic cell count in cow's milk, discloses application of the cordyceps strain fermentation product to improve cow milk yield and prepare forages for preventing cow mastitis. The application comprises that the cordyceps strain fermentation product is used to feed lactation cows. The cordyceps strain fermentation product is obtained by performing liquid fermentation or solid fermentation on a cordyceps fungus. Compared with a contrast group, a forage containing a cordyceps forage additive and used to feed lactation cows is capable of substantially improving cow milk yield, the produced cow's milk has substantially improved fat content and protein content and substantially reduced somatic cell count, and after feeding is stopped, the somatic cell count in the produced cow's milk still keeps a relatively low level. The cordyceps forage additive is shown to be capable of effectively change the health status of cows and improve the quality of the produced cow's milk, and is capable of effectively preventing cow mastitis, reducing somatic cell count in cow's milk and keeping the somatic cell count at a relatively low level for a long time.

Disclosed is a milk somatic cell counting method based on the computer vision. The method comprises the steps of dropping a coloring agent into milk, coloring somatic cells with the coloring agent, dropping the milk into a slide, and acquiring cell images by using a digital microscope, wherein the cell images are color images; preprocessing the cell images; and performing the cell image counting process: setting the somatic cell occupied pixel area range S1-S2; scanning cell binaryzation images progressively, and probing the size S of a communication area where the pixel is positioned by using a recursion method if a white pixel is found, wherein, the communication area to which the white pixel belongs is somatic cells if S1<=S<=S2, 1 is added to the somatic cell number count; otherwise, the somatic cell number count does not change; and obtaining the somatic cell number count in the cell images. According to the milk somatic cell counting method based on the computer vision, human factor influences are avoided effectively, the detection efficiency is high and the accuracy is good.

The invention provides a device and a method for flow cytometric fractionation of particles contained in a fluid stream, wherein sections of the fluid stream, especially droplets if the fluid stream is a droplet stream, are irradiated with a laser. The laser disposed for the irradiation of the sections of the fluid stream can have a wavelength which is absorbed by the fluid and can have a sufficient radiation duration and radiation intensity to deflect sections of the fluid stream.

The invention relates to a milk somatic cell counting device and a milk somatic cell counting method based on an intelligent terminal and a micro-fluidic chip. The milk somatic cell counting device is provided with the intelligent terminal for collecting an image of a fresh milk sample, a miniature optical amplification circuit with one end arranged correspondingly to a camera in the intelligent terminal and used for amplifying the fresh milk sample, and the micro-fluidic chip arranged correspondingly to the other end of the miniature optical amplification circuit and used for counting the somatic cells of the fresh milk sample in the image data. The milk somatic cell counting method comprises the following steps: diluting the fresh milk sample by 5-50 folds, carrying out staining treatment, dropwise adding 5-50 mu L of the sample into an inlet of the micro-fluidic chip; amplifying an image collection area on the micro-fluidic chip by utilizing the miniature optical amplification circuit, and photographing the image collection area by utilizing the camera of the intelligent terminal; and counting the somatic cells in the image data by utilizing matched APP application program. With the milk somatic cell counting device and the milk somatic cell counting method, somatic cells within the field of view are guaranteed to be positioned in a focal plane of the same height, focusing in the imaging process is facilitated, and a clear microscopic image is obtained.

A method for determining a concentration of at least one component of milk includes measuring impedance values between two electrodes associated with a cell containing a milk sample at each of at least three frequencies, and estimating a concentration of the at least one component from a polynomial expression in which the impedance values at the at least three frequencies are variables. A system for determining a concentration of at least one component of milk includes a sampling cell including a milk sample, electrodes operative to electrify the milk sample with excitation signals at a plurality of frequencies including at least three frequencies, a signal generator for generating the excitation signals, circuitry for determining a relationship between an amplitude and phase of reflected and incident signals obtained from the signal generator, and a processor that receives the relative amplitude and phase and estimates a concentration of at least one component of milk based on a polynomial expression relating measured impedance values at a plurality of frequencies to concentrations of at least one component of milk.

The invention relates to a cloud computing and RFID (radio frequency identification) based cow milk quality monitoring system which comprises a detection terminal, a milk detection device, a communication base station and a monitoring service cloud platform. The milk detection device comprises a milk antibiotic detection instrument, a milk somatic cell counting instrument and a milk aflatoxin M1 detection instrument; the detection terminal and the milk detection device are both arranged in each milk collection point of the milk cow pasture; the detection terminal communicates with the milk detection device through a bus interface and used for reading the detection data acquired when the milk detection device is used to detecting the milk quality and transmitting the detection data to the communication base station through a ZigBee wireless sensing network; the communication base station is arranged in the milk cow pasture and used for transmitting the diction data to the monitoring service cloud platform in the mobile communication way; and the monitoring service cloud platform is used for storing the detection data after receiving the detection data sent by the communication base station so that the user can further manage and use.

An apparatus for cleaning and pre-milking a teat of a milking animal prior to the milking animal being milked comprises a teat cleaning and pre-milking device (51) having cleaning means and pre-milking means, wherein, during cleaning and pre-milking, the teat of the animal is exposed to the cleaning and pre-milking means. The apparatus comprises further means (60; 84) for establishing that milk is actually extracted from the teat of the milking animal during the cleaning and pre-milking, means (62; 84) for measuring a quality, preferably a somatic cell count value, of the milk extracted from the teat of the animal during the cleaning and pre-milking, means for comparing the measured quality of the milk with a reference quality value, and means for indicating, depending on the comparison, whether or not milk drawn from the teat of the animal during a subsequent milking should be mixed with milk drawn from another animal.

The invention provides a feed additive for controlling recessive mastitis of a cow and reducing somatic cells and a preparation method thereof. The feed additive comprises Chinese herbal medicines, vitamins, minerals and probiotics and concretely, comprises 100 to 150g of licorice root, 50 to 100g of radix angelicae sinensis, 80 to 120g of radix astragali, 80 to 120g of pericarpium citri reticulatae, 50 to 100g of dandelion, 80 to 100g of fructus arctii, 50 to 100g of ligusticum wallichii, 100 to 150g of motherwort, 50 to 80g of Chinese violet, 60 to 100g of seed of cowherb, 50 to 80g of acanthopanax root, 50 to 80g of myrrh, 50 to 80g of white paeony root, 80 to 120g of honeysuckle, 80 to 120g of Houttuynia cordata, 50 to 100g of red sage root, 50 to 100g of pilose asiabell root, 30 to 50g of vitamin A (500000 IU / g), 20 to 30g of vitamin D3, 30 to 50g of vitamin E (5000000 IU / g), 150 to 200g of zinc hydroxymethionine, 10 to 20g of yeast selenium, 80 to 120g of saccharomycetes (20 billion cfu / g) and 10 to 20g of Bacillus licheniformis (20 billion cfu / g). The feed additive can improve the physiological functions of dairy cows, reduce the incidence of mastitis, improve the anti-oxidant capacity, reduce somatic cells and improve the quality of milk.

The invention provides an automatic smear system of a milk somatic cell counting instrument, the automatic smear system comprises a sheet inlet device, a sample inlet device, a sampling and smear device and a sheet outlet device; a path for pushing and sending the glass slide of the sheet inlet device and the path for sending a sample of the sample inlet device are orthogonal, and the path for sending the sample is positioned below the path for sending the sheet; a smear working position table is arranged at the tail end of the stroke of pushing and sending the glass slide of the sheet inlet device; and a sampling port is arranged at the smear working position table, and the tail end of the stroke of sending the sample of the sample inlet device is positioned below the sampling port. The automatic smear system reduces the motion and the corresponding motion mechanism of the sampling and smear device through the design of the upper and the lower staggered layers and the design of the same working position of the sample inlet device and the sheet inlet device, thereby not only leading the structure of the automatic smear system to be more simple, but also improving the working efficiency of the sampling and smear device; the needed sampling speed can still be met even in the situation of not adopting multiple working heads; furthermore, the automatic smear system simultaneously reduces the operation position of placing the milk sample and leading the operation of an operator to be more convenient.

The invention provides a counting method and a counting system for milk body cells based on mobile equipment. The method comprises the following steps: sample processing: marking the milk body cells for a to-be-tested milk sample; induced fluorescent amplification treatment: carrying out fluorescence enhancement on the marked milk body cells; and acquiring cell images by using mobile equipment and processing by virtue of a counting algorithm to identify the milk body cells and carry out statistics on quantity, and displaying, transmitting or printing, wherein the mobile equipment acquires images by virtue of photographing and counts the milk body cells of the images. The processing method sequentially comprises the following steps: carrying out RGB to gray processing on the images; converting the grayscale image to a binary image; carrying out breadth first search and counting; and displaying a counting result. The method and the system provided by the invention have the characteristics of small size, portability, high analysis efficiency and good precision, can be used for field detection of a milk plant and the like.

The invention relates to a single nucleotide polymorphism sequence of a dairy cattle FGF2 (Fibroblast Growth Factor 2) gene and a detection method of the single nucleotide polymorphism sequence, belonging to the technical field of genetic engineering. The single nucleotide polymorphism sequence of the dairy cattle FGF2 gene comprises a single nucleotide polymorphism sequence of which the 11646th site of a first intron of the dairy cattle FGF2 gene is A or G. The detection method comprises the following steps of: with dairy cattle genome DNA as a template and a primer pair P as a primer, carrying out PCR (Polymerase Chain Reaction) amplification on the dairy cattle FGF2 gene; and sequencing a purified PCR product to determine that the single nucleotide polymorphism 11646th site of the dairy cattle FGF2 is A or G. According to the single nucleotide polymorphism sequence, the SNP (Single nucleotide Polymorphism) site of the dairy cattle FGF2 gene is related to butterfat production, butterfat percentage, somatic cell count and reproductive capacity; and found through genotyping an externally-fertilized embryo, the SNP site is related to the embryo survival rate; and through association analysis study on FGF2 genetic polymorphism and character, the SNP site can be used for dairy cattle molecular mark assisted breeding and increase of the externally-fertilized embryo survival rate.

The invention involves using a simple colorimetric method for a quantitative test to measure white blood cells in milk samples. The invention includes a new reagent system, a new analysis method, and a new apparatus which permits in-line colorimetric analysis.

The present invention relates to a method for preparing a gene knock-out canine with use of somatic cell cloning technology, in particular relates to a method for preparing a gene knock-out canine with use of somatic cell cloning technology using a fusion liquid of low osmotic pressure together with autologous embryo transplanting.

A simple calorimetric in-line quantitative test to measure white blood cell counts in milk samples using a liquid reagent system that simplifies quantitative in-line SCC measurements using a reflectance measuring mode, and a new apparatus, which permits in-line colorimetric analysis.

The invention provides an eu-commin intragastric administration agent for cows and a production method thereof. The production method comprises the following steps: dry eucommia leaves are extracted twice continuously, and each time extraction time is 1.5-2 h; an extract liquid is subjected to stilling and precipitating by a natural clarifying agent, supernate is pumped out, lower suspension is centrifugalized, and filtrate is mixed with the supernate; an ultrafiltration membrane is separated, then a dialyzate over-wind type nanofiltration membrane is separated and condensed, dialyzate is pumped in an extracting tank for recycling, and trapped fluid is condensed till the concentration is 20-30%; nanofiltration trapped fluid is pumped in a high-temperature instant sterilization machine for sterilization, and enters into a filling machine for filling under the sterile condition. The eu-commin intragastric administration agent can enhance the immunity of the organism of the cows, effectively controls the recessive mastitis and the clinical mastitis, can reduce the somatic cell counts of the cows remarkably, improves the tendency of milk production, and has no remarkable influence on the fermentation function of cow rumen, the butter-fat content and the milk protein rate. Meanwhile, the eu-commin intragastric administration agent is an aqueous solution, is convenient to use, and has no side effects.

The invention discloses a milk somatic cell counting device based on computer vision. The device comprises a slide moving device, an image capture device, an image analyzing device and a light source which is matched with the image capture device, wherein the slide moving device comprises an upper layer moving platform and a lower layer moving platform, the upper layer platform is driven by a first stepping motor to move to the left and right, the lower layer platform is driven by a second stepping motor to move back and forth, and both the upper layer moving platform and the lower layer moving platform are arranged on an orbit; the image capture device comprises a digital microscope and a supporting fixing part of the digital microscope; the image analyzing device comprises an industrial personal computer, and the industrial personal computer is provided with an image analyzing module; and the digital microscope is connected with the industrial personal computer, the first stepping motor, the second stepping motor and the light source are connected with a single chip microcomputer, and the single chip microcomputer is connected with the industrial personal computer. According to the device, the movement of the side is controlled through control of the movement of the moving platforms, during the moving process, amplified images of milk somatic cells are collected and then analyzed, and the quantity of the milk somatic cells is obtained.

The invention discloses a gauging method of milk cell quantity, which comprises the following steps: (1) adding the color-developing agent of random commissioning ratio in the crude milk; displaying color of crude milk in the boiling-water in the strong acidic condition; preparing the color-developing agent based on DNA dyeing agent; (2) placing the crude milk with color in the colorimetric device; adapting D65 as standard light source to make the distance between sample and standard light source as 45 cm; obtaining the color information of sample through image intaking equipment; 3) counting the color information to gain the average value of corresponding color system component; 4) calculating through the algorithm formula.

The invention discloses an online milk somatic cell electrical detection device and method based on a micro-fluidic technique. The device comprises an air supply source, a reducing valve, a pressure sensor, a liquid container, a throttling valve, a T-shaped micro-channel, micro detection electrodes, a capacitive sensor and a microprocessor, wherein the reducing valve is communicated with the air supply source and the liquid container; the liquid container is connected with the throttling valve; the throttling valve is communicated with the T-shaped micro-channel; the micro detection electrodesare arranged on the upper and lower sides of the T-shaped micro-channel in pairs; the micro detection electrodes are connected with the capacitive sensor; the capacitive sensor is connected with themicroprocessor; the pressure sensor is connected with the microprocessor; the microprocessor is connected with the throttling valve. According to the device disclosed by the invention, the micro-fluidic chip, the micro detection electrodes and the micro-fluidic flow regulation device are integrated into a whole, the detection accuracy of the milk somatic cell count can be improved, the detection cost is reduced, and online quantitative determination of the milk somatic cell count is realized.

A method is provided for the discrimination of cells from other particles, as well as of different types of cells in raw milk samples by impedance microflow cytometry. A method is provided of analysing the quality of raw milk in terms of its bacterial and somatic cell content without the need to pre-treat the milk sample, such that the analysis can take place directly at the production site. One advantage is discriminating and counting somatic cells from milk particles (consisting mainly of lipid vesicles) by high-frequency impedance analysis directly performed on untreated raw milk. Another advantage is that the method allows diagnosing the status of a mastitis infection directly after milking according to the analysis of the somatic cell count. Another advantage is to allow a fast method for determining the bacterial cell count in raw milk directly after milking. In addition, viability of both somatic and bacterial cells can be determined without the need of any cell label. A further advantage is, that the analysis can be obtained in real-time, directly after the raw milk has passed the micro channel of the microfluidic device.

The present invention provided a method for predicting a phenotype in a bovine animal, the method comprising analysing a nucleic acid sample from said animal for the presence of at least one genetic marker known to reside in an Linkage Disequilibrium (LD) block in any one of bovine chromosomes BTA-I to BTA-29, wherein said LD block is associated with said phenotype. The phenotype can be Australia profit ranking (APR), Australian selection index (ASR), protein yield (PROT), protein percent (PROT %), milk volume (MILK), fat yield (FAT), fat percent (FAT %), breeding value overall type (Overall Type), somatic cell count (SCC), and / or breeding value cow fertility (Cow Fertility). Also provided is a linkage disequilibrium unit (LDU) map of any one or more of bovine chromosomes BTA-I to BTA-29, the map comprising a plurality of chromosomal regions, and the regions defined by their co-inheritance across generations substantially as entire linkage disequilibrium (LD) blocks.

The invention discloses a Chinese herbal preparation for preventing and treating dairy cow subclinical mastitis and reducing milk SCC. The Chinese herbal preparation is composed of, by weight part, 6parts of herba taraxaci, 6 parts of herba violae, 4 parts of fructus forsythia, 3 parts of flos lonicerae, 2 parts of rhizoma coptidis, 2 parts of radix paeoniae rubra, 3 parts of radix codonopsis, 3parts of radix angelicae sinensis, 3 parts of fructus trichosanthis and 2 parts of radix glycyrrhizae. The Chinese herbal preparation for preventing and treating dairy cow subclinical mastitis and reducing milk SCC is reasonable in design, and through research on compound traditional Chinese medicines for preventing and treating dairy cow subclinical mastitis and reducing milk SCC, improves the preventing and treating effects of Chinese herbs on dairy cow subclinical mastitis and provides a theoretical basis for comprehensive prevention and treatment on dairy cow subclinical mastitis and reduction of milk SCC with Chinese herbs.

The invention discloses a feed additive for dairy cow concentrate supplement feed, and belongs to the technical fields of animal nutrition and feed. The feed additive comprises arazyme, zinc methionine, magnesium sulfate and sodium diacetate, can improve the milk yield of dairy cow, reduce the somatic cell count in milk, and has certain prevention and treatment effects on dairy cow mastitis.

The invention discloses a micro-fluidic chip used for raw milk body cell counting. The micro-fluidic chip comprises a micro-fluidic channel unit plate arranged on a substrate, wherein a group of micro-fluidic channels for bearing a measured raw milk sample fluid is formed on the micro-fluidic channel unit. Each micro-fluidic channel comprises an inlet for injecting a pre-treated to-be-measured raw milk sample fluid, 2-6 channels which are connected on the inlet at certain ends and are communicated with the inlet, wherein each of the other ends of the 2-6 channels is connected with a fluid collecting cavity for collecting a detected waste liquid; 2-6 micro cavities which are communicated with the channel for placing the to-be-measured raw milk sample fluid are formed in each channel at equal intervals. The distribution, in the micro cavities, of the body cells is limited by the height of the micro cavities, so that the body cells within the field of view are guaranteed to be almost in a focal plane at the same height, and thus, focusing in an imaging process is facilitated to form a clear microscopic image, and accurate counting is realized. The micro-fluidic chip can be directly applied to counting raw milk body cells by a microscopic method, and can be used for remarkably improving the detection precision.

A simple, rapid and accurate method for detecting nucleic acids from milk are disclosed. The methods are useful in determining somatic cell count levels and diagnosing mastitis in cows.

The invention relates to the field of livestock breeding, in particular to a medicament for reducing somatic cell count in milk and a preparation method. The milk produced by treated cows does not contain medicine residues, so that the quality of the milk is improved. The medicament for reducing somatic cell count in milk comprises the following main ingredients: 90-110 parts of raw astragalus membranaceus processed with honey, 50-70 parts of codonopsis pilosula, 30-50 parts of bighead atractylodes rhizome, 20-40 parts of fructus schisandrae, 20-40 parts of medlar, 20-40 parts of jujube, 50-70 parts of radix scutellariae processed with wine, 50-70 parts of sophora and 10-30 parts of tropaeolum. The preparation method of the medicament for reducing somatic cell count in milk specifically comprises the following steps of: mixing the raw astragalus membranaceus processed with honey, the codonopsis pilosula, the bighead atractylodes rhizome, the fructus schisandrae, the medlar, the jujube, the radix scutellariae processed with wine, the sophora and the tropaeolum, and crushing into 60-80-mesh fine powder; adding water into the prepared fine powder, and evenly stirring; implanting saccharomycetes; fermenting for 12-14 hours; and drying.