Application of cordyceps strain fermentation product to reduce somatic cell count in cow's milk
A technology of bovine milk somatic cells and Cordyceps genus, applied in application, animal feed, animal feed, etc., can solve the problems of dairy cow application, milk fat rate, milk protein rate decrease, calcium content decrease, etc., and achieve the prevention of dairy cow mastitis , reduce the number of somatic cells, and increase the protein content
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Embodiment 1
[0045] Example 1 Application of Acremonium acremonium culture (Cordyceps Xinkang) in dairy cow production
[0046] 1. Preparation of Acremonium acremonium culture (bacteria liquid, bacteria powder, solid culture)
[0047] (1) Preparation of Acremonium acremonium liquid fermentation liquid
[0048] ①Gunni Cordyceps strain: Acremonium terricola MKL18
[0049] ② Liquid fermentation medium
[0050] First-class seed liquid fermentation medium: 600g white sugar, 200g yeast powder, 10g MgSO 4 , 10g KH 2 PO 4 , dissolved in 20L water, natural pH; sterilized by high-pressure steam at 121°C for 15 minutes, and set aside.
[0051] Secondary seed liquid fermentation medium: 6000g white sugar, 2000g soybean powder, 2000g yeast powder, 100gMgSO 4 ,100g KH 2 PO 4 , dissolved in 200L water, natural pH; sterilized by high-pressure steam at 121°C for 15 minutes, and set aside.
[0052] ③ Liquid fermentation
[0053] Liquid seed fermentation: liquid fermentation control parameters are ...
Embodiment 2
[0169] Example 2 Application of Acremonium acremonium culture (Cordyceps Xinkang) in combination with other additives in dairy cow production
[0170] The above-mentioned Acremonium acremonium culture is mixed with different feed additives to prepare a mixed feed additive. There are many additives that can be mixed with the Acremonium acremonium culture, such as: GABA, motherwort, honeysuckle, forsythia, Dandelions, parsnips and more. The following uses GABA and Motherwort as examples to illustrate that Acremonium acremonium culture can be combined with various feed additives and applied in dairy cow production.
[0171] Take GABA and Leonurus fine powder (100 mesh), mix them with the solid fermentation culture of Acremonium acremonium respectively in a ratio of 50:50, and prepare a mixed feed additive for testing, namely: GABA-containing Acremonium acremonium culture mixed feed additive, acremonium acremonium culture mixed feed additive containing motherwort. Add 0.05%, 0.1...
Embodiment 3
[0213] Example 3 Application of the culture of Cordyceps militaris in dairy cow production
[0214] 1. The culture medium formula and culture of Cordyceps militaris
[0215] (1) Cordyceps militaris strain: Paecilomyces militaris;
[0216] (2) culture medium
[0217] ① Slant medium: PDA medium, that is, potato dextrose agar medium. 200g peeled potatoes (boiled juice), 20g glucose, 1g potassium dihydrogen phosphate, 20g agar, 1000ml water. Natural pH.
[0218] ② Liquid seed medium: 2.0% sucrose, 0.4% peptone, 0.3% yeast extract powder, 0.5% corn flour, MgS0 4 7H 2 O 0.02%, K 2 HP0 4 0.04%. Natural pH.
[0219] ③Solid medium: potato starch 83%, corn flour 10%, white sugar 2%, peptone 2%, silkworm chrysalis powder 2.5%, magnesium sulfate 0.2%, ammonium citrate 0.2%, potassium dihydrogen phosphate 0.1%, vitamin B 1 100mg, water PH value 5-7.
[0220] (3) Fermentation method:
[0221] ① Liquid fermentation
[0222] Liquid seed fermentation: liquid fermentation control p...
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