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Milk somatic cell counting device and method based on intelligent terminal and micro-fluidic chip

A technology of microfluidic chips and milk somatic cells, which is applied in the fields of measuring devices, individual particle analysis, particle and sedimentation analysis, etc., can solve problems such as cumbersome operation steps, color depth dependence, and influence on the reliability of test results, etc., to achieve Low detection cost, easy operation, and easy on-site detection

Inactive Publication Date: 2015-09-09
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese application 201010173697.4 (milk somatic cell detection kit and method) mentions a test kit composed of sample pad, colloidal gold pad, nitrocellulose membrane, water-absorbing filter paper, reaction support, etc. Colloidal gold markers are required in the detection process Dairy cow somatic cell monoclonal antibody and other IgG to complete the color development, and the operation steps are cumbersome
Chinese application 201220232862.3 (milk somatic cell 4-hole detection test paper) provides a sandwich-structured paper-based detection test paper for milk somatic cell detection, but the color depth of this method depends on the reaction substrate and corresponding reaction enzyme on the test paper , and the content of somatic cells must be estimated with the help of a spectrophotometer, which is an indirect test method, which affects the reliability of the test results

Method used

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  • Milk somatic cell counting device and method based on intelligent terminal and micro-fluidic chip
  • Milk somatic cell counting device and method based on intelligent terminal and micro-fluidic chip
  • Milk somatic cell counting device and method based on intelligent terminal and micro-fluidic chip

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example 1

[0077](1) Preparation of staining agent and dilution of milk samples: Add 150uL iodine tincture dropwise to each milliliter of normal saline, invert and mix evenly, and prepare staining agent. Mix the milk sample with the above staining agent at a ratio of 1:19 by volume, and shake it upside down.

[0078] (2) Solution sampling: Add 1uL of the above-mentioned diluted solution dropwise to the injection port of the microfluidic chip, and the observation cavity is filled with the solution to be detected.

[0079] (3) Milk somatic cell imaging: build a microscopic magnification optical path with a double-convex lens, adjust the magnification to 60 times, and use the smartphone camera to clearly image each observation cavity.

[0080] (4) Image and data processing: Call the APP installed on the mobile phone to obtain relevant data such as the concentration of milk somatic cells and the probability of mastitis in dairy cows, and use the network to share the test results.

example 2

[0082] (1) Pretreatment of milk samples. The raw milk sample was diluted 30 times and stained with tincture of iodine, and then 2uL of the sample was added dropwise to the inlet of the microfluidic chip.

[0083] (2) Image enlargement and acquisition. The image sampling area on the microfluidic chip is magnified by using a micro-optical amplification circuit (magnification factor of 100 times), and the camera of the smart terminal is used to take pictures of it.

[0084] (3) Data processing and result display. Using the supporting APP application program, the somatic cells in the image data are counted and processed, and the relevant data such as the concentration of somatic cells and the probability of mastitis in dairy cows are converted, and the detection results are shared through the network.

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Abstract

The invention relates to a milk somatic cell counting device and a milk somatic cell counting method based on an intelligent terminal and a micro-fluidic chip. The milk somatic cell counting device is provided with the intelligent terminal for collecting an image of a fresh milk sample, a miniature optical amplification circuit with one end arranged correspondingly to a camera in the intelligent terminal and used for amplifying the fresh milk sample, and the micro-fluidic chip arranged correspondingly to the other end of the miniature optical amplification circuit and used for counting the somatic cells of the fresh milk sample in the image data. The milk somatic cell counting method comprises the following steps: diluting the fresh milk sample by 5-50 folds, carrying out staining treatment, dropwise adding 5-50 mu L of the sample into an inlet of the micro-fluidic chip; amplifying an image collection area on the micro-fluidic chip by utilizing the miniature optical amplification circuit, and photographing the image collection area by utilizing the camera of the intelligent terminal; and counting the somatic cells in the image data by utilizing matched APP application program. With the milk somatic cell counting device and the milk somatic cell counting method, somatic cells within the field of view are guaranteed to be positioned in a focal plane of the same height, focusing in the imaging process is facilitated, and a clear microscopic image is obtained.

Description

technical field [0001] The invention relates to a milk somatic cell counting device and method. In particular, it relates to a milk somatic cell counting device and method based on an intelligent terminal and a microfluidic chip. Background technique [0002] Milk somatic cell count (SCC) refers to the total number of cells per milliliter of milk, most of which are white blood cells, accounting for about 95% of the somatic cell count. The somatic cell count reflects the quality of milk and the health of dairy cows. Under normal circumstances, the number of somatic cells in milk is generally 20,000 to 200,000 / mL. If its content exceeds 500,000 / mL, the cows will show clinical symptoms of mastitis. When the number of somatic cells is between 200,000 and 500,000 / mL, cows often suffer from invisible mastitis, which is difficult to detect in terms of symptoms, but seriously affects the quality and taste of fresh milk. [0003] Current technologies mostly use direct microscopy, ...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 李姗姗闫姿姿李铁军戴士杰
Owner HEBEI UNIV OF TECH
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