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DNA fluorescence in situ hybridization BCR/ABL fusion state detection method and detection system

A fluorescence in situ hybridization and state detection technology, applied in the field of image analysis, can solve the problems of being unable to apply to actual scenarios and slow time consumption, and achieve the effect of automatic detection, high detection efficiency, and promotion of the transformation of diagnostic and therapeutic instruments

Active Publication Date: 2022-07-22
昆明金域医学检验所有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The traditional method of manually counting and diagnosing BCR / ABL fusion status is time-consuming and slow;
[0008] At present, the BCR / ABL fusion state detection method based on CNN neural network automation cannot be applied to actual scenarios due to equipment and other limitations.

Method used

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  • DNA fluorescence in situ hybridization BCR/ABL fusion state detection method and detection system
  • DNA fluorescence in situ hybridization BCR/ABL fusion state detection method and detection system
  • DNA fluorescence in situ hybridization BCR/ABL fusion state detection method and detection system

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Embodiment

[0098] Materials and methods

[0099] Slide preparation, probe hybridization and image acquisition.

[0100] The bone marrow sample was obtained from a medical laboratory.

[0101] Slide preparation and probe hybridization are as follows:

[0102] 1. Mix the sample upside down, take 2ml into a 15ml EP tube, centrifuge at 2200rpm for 4min, (if the blood is not enough to 2ml, do not need to centrifuge, directly add 8ml of 0.075N kcl solution preheated at 37 degrees to the EP tube, when adding Wash the blood collection tube, mix it with a pipette, blow about 100 times).

[0103] 2. Aspirate and discard the supernatant, add 8 ml of 0.075N kcl solution preheated at 37°C to the EP tube, mix with a pipette (about 100 times), and place it in a 37°C water bath for hypotonic treatment for 40min.

[0104] 3. Add 2 ml of fixative solution (the fixative solution is prepared as glacial acetic acid: methanol = 1:3), mix by pipetting (about 40 times), and centrifuge at 2200 rpm for 7 min. ...

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Abstract

The invention belongs to the technical field of image analysis, and discloses a DNA fluorescence in situ hybridization BCR / ABL fusion state detection method and a detection system, which are characterized in that a CNN identification model is utilized to generate pseudonucleus staining of cells from a phase difference image, and FISH cell nucleuses are positioned and classified; and positioning and classifying each independent fluorescence signal, and dividing the number of color development points of the same classification in each photo by the total number of display points in the photo to obtain a BCR / ABL fusion ratio. The invention provides a system for detecting the fusion level of cell nucleuses and BCR / ABL genes by analyzing fluorescence in situ hybridization (FISH) images and calculating the image ratio of the number of abnormal cell nucleuses related to all classified cell nucleuses as an index for classifying the BCR / ABL gene fusion states of corresponding tumor samples. The detection method is high in detection efficiency, accurate in detection result and capable of achieving automatic detection.

Description

technical field [0001] The invention belongs to the technical field of image analysis, and in particular relates to a DNA fluorescence in situ hybridization BCR / ABL fusion state detection method and detection system. Background technique [0002] Currently, DNA fluorescence in situ hybridization (FISH) is one of the tools to study genome fusion, rearrangement and amplification because it directly visualizes the location of gene loci in the 3D space of cells. Traditional DNA FISH uses enzyme-labeled fluorescent probes that hybridize to genomic regions of interest in a sequence-specific manner. [0003] Pathologists analyzed blood tumor samples for BCR / ABL gene fusion status by evaluation with control samples. Detection criteria were defined as BCR / ABL positive status when there was evidence of BCR / ABL gene fusions (equivalent to >10% of contiguous and homogeneous tumor cell nuclei observed within the tumor area) based on counts of at least 200 nuclei within the area. By ...

Claims

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Application Information

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IPC IPC(8): G06T7/00G06T7/12G06K9/62G06N3/04G06N3/08G06V10/764G06V10/80G06V10/82
CPCG06T7/0012G06T7/12G06N3/08G06T2207/10056G06T2207/20152G06N3/045G06F18/241G06F18/254Y02A90/10
Inventor 陈武龙陈欣魏彩霞吴鹏春郅宏芳曾敬富章辉
Owner 昆明金域医学检验所有限公司
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