Yellow fluorescence carbon dots with high quantum yield and preparation method thereof

Abstract

The invention relates to yellow fluorescence carbon dots with high quantum yield. A preparation method of the yellow fluorescence carbon dots is characterized by comprising the following steps: dissolving organic acid and organic amine in water; performing microwave radiation after fully mixing the organic acid and the organic amine, thus obtaining a brown solid crude product; performing reverse-phase silica gel column chromatography purification by taking a mixed solution of the water and methyl alcohol as an eluting agent, thus obtaining the yellow fluorescence carbon dots. The yellow fluorescence carbon dots are characterized in that the average grain diameter is 3.0 to 4.0 nm; bright yellow fluorescent light can be emitted under the irradiation of an ultraviolet lamp, the excitation wavelength is 400 to 420 nm, the emission wavelength is 510 to 550 nm, the luminescent property of solids is shown, and the quantum yield can reach 44 percent. The yellow fluorescence carbon dots prepared by the invention are good in water solubility and stable in fluorescence signals, are suitable for cell imaging under physiological conditions, have a function of nuclear staining and can be widely applied to the fields of analysis and detection, biochemical sensing, biological imaging, biological marking and the like.

Description

Technical field [0001] The invention relates to a fluorescent carbon nano material, in particular to a yellow fluorescent carbon dot with high quantum yield and preparation thereof, and is mainly used for bioimaging and biomarking under physiological conditions. Background technique [0002] As a new member of the carbon material family, carbon dots are one of the most popular carbon nanomaterials after fullerenes, carbon nanotubes, and graphene. Carbon dots are usually composed of four basic elements: C, H, O, and N. The particle size is generally less than 10 nanometers. It has good optical properties, adjustable luminescence range and good light stability. Compared with traditional organic dyes and semiconductor quantum dots, fluorescent carbon dots have good water solubility and chemical stability, easy functionalization, low cytotoxicity and excellent biocompatibility. Once discovered, they have caused great His research interests have shown great application potential in t...

AI Technical Summary

Problems solved by technology

[0003] However, there are still two key problems in the research of carbon dots: first, the quantum yield of fluorescent carbon dots is low and needs to be improved urgently; second, most fluorescent carbon dots emit blue fluorescence, and the emission wavelength is longer There are few reports of fluorescent carbon dots

Since the autofluorescence of biological tissues is mostly blue, blue fluorescent carbon dots are not conducive to the distinction between target signals and background signals when applied to biological imaging; in addition, blue light has poor penetration into tissues, which limits the optical properties of deep tissues in vivo. imaging

Although some excitation wavelength-dependent fluorescent carbon dots can adjust the emission wavelength by adjusting the excitation wavelength, the quantum yield of non-blue light emission decreases rapidly, which limits its application in biology.

Images