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Adipose-derived stem cell loaded nerve conduit and preparation method thereof

An adipose stem cell and nerve conduit technology, which is applied in the field of nerve conduit loaded with adipose stem cells and its preparation, can solve the problems of inability to achieve neural tissue structure remodeling, insignificant effect of material-cell interface, and low regeneration and repair ability of Schwann cells. , to achieve the effect of favoring adhesion and proliferation, increasing specific surface area and cell adhesion sites, and promoting myelination

Active Publication Date: 2022-07-12
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the current tissue engineering nerve scaffold materials can achieve directional topology and provide continuous and effective electrical signal stimulation to induce Schwann cell growth, migration and arrangement, however In long-segment peripheral nerve injury, the regeneration and repair ability of Schwann cells is low, the effect of material-cell interface is not obvious, and the reconstruction of nerve tissue structure cannot be realized

Method used

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  • Adipose-derived stem cell loaded nerve conduit and preparation method thereof
  • Adipose-derived stem cell loaded nerve conduit and preparation method thereof
  • Adipose-derived stem cell loaded nerve conduit and preparation method thereof

Examples

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Effect test

Embodiment 1

[0041] The present embodiment provides a method for preparing a nerve conduit, the steps comprising:

[0042] S1, add 1g of polycaprolactone and 0.8g of polyvinylpyrrolidone into 10mL of dichloromethane / dimethylformamide organic solvent (the volume ratio of dichloromethane and dimethylformamide is 3:1), ultrasonically treated for 30 After one minute, add 2% (w / w) reduced graphene oxide nanoparticles to obtain a spinning solution;

[0043] S2, adding the spinning solution obtained in step S1 into the syringe, the receiving rod receiving distance is 15cm, the rotating speed is 10rpm, the voltage of 15kV is applied, the speed of the propelling pump is 2.5mL / h, and the negative pressure at both ends of the insulating rod is- 1.5kV, after electrospinning, washing with alcohol and water for 3 times to remove polyvinylpyrrolidone, the groove structure can be obtained on the surface of the fiber, and the semi-finished catheter is obtained;

[0044]S3. Inject the cell mixture into the...

Embodiment 2

[0046] The present embodiment provides another method for preparing a nerve conduit, the steps comprising:

[0047] S1, add 1g of polycaprolactone and 0.4g of polyvinylpyrrolidone into 10mL of dichloromethane / dimethylformamide organic solvent (the volume ratio of dichloromethane and dimethylformamide is 3:1), ultrasonically treated for 20 10 minutes later, add 1% (w / w) reduced graphene oxide nanoparticles to obtain a spinning solution;

[0048] S2, adding the spinning solution obtained in step S1 into the syringe, the receiving rod receiving distance is 15cm, the rotating speed is 10rpm, the voltage of 15kV is applied, the speed of the propelling pump is 2.5mL / h, and the negative pressure at both ends of the insulating rod is- 1.5kV, after electrospinning, washing with alcohol and water for 3 times to remove polyvinylpyrrolidone, the groove structure can be obtained on the surface of the fiber, and the semi-finished catheter is obtained;

[0049] S3. Inject the cell mixture i...

Embodiment 3

[0051] The present embodiment provides another method for preparing a nerve conduit, the steps comprising:

[0052] S1, add 1.2g polycaprolactone and 0.8g polyvinylpyrrolidone to dichloromethane / dimethylformamide organic solvent (the volume ratio of dichloromethane and dimethylformamide is 3:1), ultrasonically treat 40 Minutes later, adding 2.5% (w / w) reduced graphene oxide nanoparticles to obtain a spinning solution;

[0053] S2, adding the spinning solution obtained in step S1 into the syringe, the receiving rod receiving distance is 15cm, the rotating speed is 100rpm, the voltage of 15kV is applied, the speed of the propelling pump is 2.5mL / h, and the negative pressure at both ends of the insulating rod is- 1.5kV, after electrospinning, washing with alcohol and water for 3 times to remove polyvinylpyrrolidone, the groove structure can be obtained on the surface of the fiber, and the semi-finished catheter is obtained;

[0054] S3. The inner wall of the semi-finished cathet...

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Abstract

The invention provides a nerve conduit loaded with adipose-derived stem cells and a preparation method thereof.The preparation method comprises the steps that S1, polycaprolactone and polyvinylpyrrolidone are added into a binary organic solvent, after ultrasonic treatment, reduced graphene oxide nanoparticles are added, and a spinning solution is obtained; s2, carrying out electrostatic spinning on the spinning solution by utilizing an electrostatic spinning technology, and washing for a plurality of times to obtain a semi-finished conduit product; and S3, injecting the cell mixture into the semi-finished conduit product to obtain the nerve conduit, the surface of the adipose-derived stem cell-loaded nerve conduit fiber provided by the invention has a groove structure, so that the specific surface area and cell adhesion sites are increased, and adhesion and proliferation of cells are facilitated; by loading the adipose-derived stem cells, the neurotrophic phenotypic effect of the peripheral nerve scaffold is improved, and the adipose-derived stem cells can effectively avoid immunological rejection of transplantation and promote directional growth of axons into nerve conduits and the myelination effect of Schwann cells.

Description

technical field [0001] The invention relates to the field of electrospinning scaffolds, in particular to a nerve conduit loaded with adipose stem cells and a preparation method thereof. Background technique [0002] Peripheral nerve injury is a high-incidence and intractable disease in clinical practice. Due to the mismatch of nerve stumps and the slow growth rate of long-segment peripheral nerve injury, the repair efficiency is often low, resulting in motor and sensory dysfunction of patients, and even teratogenicity and disability in severe cases. For smaller defects, peripheral nerves have a certain regeneration ability and can repair the defect by themselves. The treatment of long-segment peripheral nerve defects is quite challenging. At present, the clinical gold standard is still nerve transplantation. However, this treatment has important defects such as donor limitation, size mismatch, and damage to the donor site, and long-term follow-up found that patients The fun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L29/00A61L29/02A61L29/04A61L29/06A61L29/16A61M25/00D01D5/00
CPCA61L29/06A61L29/041A61L29/005A61L29/16A61L29/02A61M25/0009D01D5/0069A61M2025/006A61L2300/10A61L2300/30A61L2300/412A61L2400/12A61L2430/32C08L67/04C08L39/06A61L27/443A61L27/3878A61L27/383
Inventor 钱运范存义姚湘云欧阳元明黄晨詹雷严至文蒋慧荃王旭孔令驰姚之肖王师堃
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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