The invention relates to a cell culture medium. The cell culture medium contains a basal culture medium, a serum substitute, linoleic acid, testosterone, vitamin A, L-ascorbic acid, polylysine, putrescine, and 2-mercaptoethanol. The cell culture medium of the invention can efficiently culture sebaceous gland cells in vitro, so that a cell proliferation rate, a survival rate after resuscitation, and an expression amount of lipid synthesis related proteins of different generations of the sebaceous gland cells are significantly increased, and thereby the assistance for extensively researching sebaceous gland physiological and endocrine functions, exploring pathogenesis of sebaceous gland related diseases, and developing drugs is provided.