32results about How to "Enhance in vitro proliferative ability" patented technology

The invention relates to a culture medium containing human umbilical cord mesenchymal stem cell exudates and a preparation method and applications thereof. An umbilical cord mesenchymal stem cell finite cell line is established to identify biological characteristics of the umbilical cord mesenchymal stem cell, and the preparation method for the human umbilical cord mesenchymal stem cell exudates is established to verify that the human umbilical cord mesenchymal stem cell (HUCMSC) exudates can promote cell regeneration, improve cell function and suppress cell apoptosis in vitro. A detection of enzyme-linked immunosorbent assay (ELISA) shows that human umbilical cord mesenchymal stem cell exudates contain abundant cell factors which can promote cell proliferation and suppress cell apoptosis, such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF).

The invention relates to a skin renewal method. According to the skin renewal method, hair follicle stem cells are used as seed cells and silica gel dressing is used as a new cell transplantation carrier. The skin renewal method comprises the following steps: 1) cultivating and amplifying hair follicle stem cells; 2) cultivating dermal cells; 3) mixing the hair follicle stem cells cultivated and amplified in the step 1) and the dermal cells cultivated in the step 2); 4) dropwise adding mixed cells obtained in the step 3) into silica gel dressing for incubation; and 5) transplanting the cells and the silica gel dressing used in the step 4) to a part required to be subjected to skin renewal. The method provided by the invention not only can renew functional skin in the treatment of large-area skin injuries, but also can provide a simple validation method for the multipotency test on hair follicle stem cells in scientific research activities.

The invention relates to the field of a preparation method and application of a traditional Chinese medicine effective component and in particular relates to a preparation method of rhizoma polygonatipolysaccharide and application of the rhizoma polygonati polysaccharide to the preparation of an immunological enhancement medicine. The rhizoma polygonati polysaccharide is obtained by processing raw rhizoma polygonati, extracting and separating; when an extraction method provided by the invention is adopted, the extraction rate of the rhizoma polygonati polysaccharide is high and the content oftotal polysaccharide is high; the proliferation activity of the rhizoma polygonati polysaccharide on normal mouse spleen cells is improved; cyclophosphamide induced spleen and thymus gland index of mice with low immunity also can be improved; the in-vitro proliferation capability of spleen and thymus gland cells under the stimulation of concanavalin A is enhanced; the phagocytic function of macrophage of the mice with the low immunity and the capability of secreting IL-6 and TNF-alpha are enhanced; the rhizoma polygonati polysaccharide has actual application value in the aspect of treatment of diseases including immunodeficiency diseases, tumors and the like.

The invention provides a fourth-generation CAR-T cell and an application thereof. The fourth-generation CAR-T cell expresses a chimeric antigen receptor specifically binding to an antigen, a secretoryIL-7 and an NFAT regulatory CCL19,wherein the signal peptide of the IL-7 is an IL-10 signal peptide; and a promoter of the CCL19 is an NFAT regulatory promoter. According to the invention, the original signal peptide of the IL-7 is changed into the signal peptide of T cell secretory protein IL-10, and the NFAT regulatory promoter is adopted as the promoter of CCL19, so that the CAR-T cell can secrete the IL-7 to the outside of the cell and conditionally express the CCL19, the proliferation of the CAR-T cell is promoted, immune cells are recruited to the inside of tumors to the greatest extent, and the anti-tumor efficacy of the CAR-T is obviously improved.

The invention relates to a method for slowing down ovarian surface epithelial cell vicious transformation. During ovarian surface epithelial cell vicious transformation, active vitamin D (1,25(OH)2D3) is added through each passage and after each culture medium replacement so as to slow down the ovarian surface epithelial cell vicious transformation. The invention further provides application of the vitamin D in the preparation of medicine for treating ovarian cancer. By the long-term use of low dosage of vitamin D, 25-hydroxyvitamin D or active vitamin D, the tumorigenic ability of ovarian surface epithelial cells in the body of a nude mouse can be weakened effectively.

The invention relates to a T cell proliferation method and an application thereof, and belongs to the field of biomedicine. The method comprises the following steps: obtaining a cell cellosilk, wherein the cell cellosilk comprises an inner core and a fiber outer layer wrapping the inner core; wherein the inner core is derived from a cell suspension containing T cells, and the fiber outer layer is derived from a high polymer material; and culturing the cell cellosilk to obtain the T cell containing the fiber outer layer. According to the T cell proliferation method provided by the invention, the in-vitro proliferation capability of the T cells is improved, the survival rate of the T cells is increased, a new thought is provided for in-vitro culture of the T cells, and a new cell culture method is provided for adoptive immunotherapy.

The invention discloses a method for preparing an isolated hair follicle in a process of treating scar by hair follicle stem cell transplantation. The method comprises the following steps: extractionof hair follicles, separation of the hair follicles, completely separating the hair follicle units into single hair follicles under an electron microscope, and keeping the extracted skin appendages such as sebaceous glands, sweat glands and the like as far as possible; for scars in a smooth hairless area, removing lower 1 / 3 of hair papilla, so that the hair follicle could not enter the next growthcycle, and no final hair grows in the scar area; in vitro culture of the hair follicles, putting the isolated single hair follicle into a special culture solution for culturing for later use; planting the hair follicle stem cells in a specific area of the scar on the basis of hair follicles. According to the method, through a hair follicle in-vitro culture technology, the single hair follicle isseparated and inactivated by adopting a separation method different from a hair transplantation operation, and is cultured in the special culture solution, so that growth and proliferation of hair follicle stem cells are facilitated. The method can achieve purposes of inhibiting scar hyperplasia, softening and repairing scars, and removing and beautifying scars.

The invention relates to the field of protein, in particular to bioactive polypeptide LPYPYYA as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide LPYPYYA is Leu-Pro-Tyr-Pro-Tyr-Tyr-Ala. In vitro immune function regulation experiments and in vivo anti-aging experiments prove that the polypeptide LPYPYYA has better immune regulation functionand anti-aging activity; on one hand, the bioactive polypeptide LPYPYYA can enhance the in vitro multiplication capacity of lymphocytes and macrophages, improve the capability of the body for resisting external pathogen infection and reduce the disease incidence of the body; on the other hand, the activity of an in vivo antiperoxidase system can be improved; the function of the body for resistingthe exogenous irritation is enhanced, so that the body aging, senility and sickness probability is reduced; very important significance is realized on developing food, health products and medicines with the immune regulation function and the anti-aging function.

The invention discloses a technical method for treating scars by hair follicle stem cell transplantation. The technical method comprises the following steps: treatment of scar areas; Extraction and separation of hair follicles; in vitro-culture of the hair follicles; wherein specially-prepared anesthetics are used, and through a preparation change and innovation of scar anesthetics, the purposes of improving scar blood supply, softening scars and facilitating the growth of hair follicle stem cells are achieved; and transplantation of the hair follicle stem cells. Through in-vitro culture technology of the hair follicles, FUE technology is adopted to extract and separate single hair follicle, the single hair follicle is put into a special culture solution to be cultured, and a special anesthetic is prepared and used, thus achieving the purposes of improving scar blood supply, softening scars and facilitating growth of hair follicle stem cells, thus finally realizing the effects of inhibiting scar hyperplasia, softening and repairing the scars and removing and beautifying the scars.

The invention relates to a culture medium containing human umbilical cord mesenchymal stem cell exudates and a preparation method and applications thereof. An umbilical cord mesenchymal stem cell finite cell line is established to identify biological characteristics of the umbilical cord mesenchymal stem cell, and the preparation method for the human umbilical cord mesenchymal stem cell exudates is established to verify that the human umbilical cord mesenchymal stem cell (HUCMSC) exudates can promote cell regeneration, improve cell function and suppress cell apoptosis in vitro. A detection of enzyme-linked immunosorbent assay (ELISA) shows that human umbilical cord mesenchymal stem cell exudates contain abundant cell factors which can promote cell proliferation and suppress cell apoptosis, such as hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (bFGF).

The invention relates to the field of proteins, and particularly relates to a bioactive peptide SAPRHGSLGFLPRK and a preparation method and application thereof. The amino acid sequence of the bioactive peptide SAPRHGSLGFLPRK is Ser-Ala-Pro-Arg-His-Gly-Ser-Leu-Gly-Phe-Leu-Pro-Arg-Lys. In-vitro immune regulation function verification experiments show that the bioactive peptide SAPRHGSLGFLPRK has a good immune regulation function. The bioactive peptide SAPRHGSLGFLPRK has a remarkable promotion effect on the in-vitro proliferation ability of macrophages, and meanwhile the ability of promoting the nitric oxide induction amount is improved, so that the ability of a body to resist external pathogen infection is improved, the morbidity of the body is reduced, the life quality is improved, and the bioactive peptide has very important significance in developing food, health care products and medicine with the immune regulation function.

The invention provides a construction method for a hepatic progenitor cell-like cell bank. The method includes the following steps: performing transformation culture on human primary hepatocyte cultures derived from different donors, performing cryopreservation treatment, performing propagation culture, performing primary passage treatment, performing virus infection, performing secondary passagetreatment, performing continuous screening culture and performing continuous passage culture. According to the construction method for the heterogeneous immortal hepatic progenitor cell-like cell bankprovided by the invention, the human primary hepatocyte cultures derived from the donors are firstly subjected to transformation culture before proliferation culture, so that the method is beneficialto giving good proliferation performance to the human primary hepatocyte cultures, and culture parameters are controlled in the subsequent stage, so that obtained immortal hepatic progenitor cell-like cell lines derived from the different donors have good in-vitro proliferation ability. The invention also provides an application of the liver progenitor cell-like cell bank and the cell lines obtained by the construction method.

The invention discloses a method for preparing and using hair follicle stem cell regeneration tissue mud in the process of treating concave scars through hair follicle stem cell and hair follicle surrounding skin regeneration tissue transplantation. The method comprises the following steps: 1, extracting a complete hair follicle unit; 2, separating hair follicle stem cells and skin regeneration tissues around hair follicles; 3, in-vitro culture and activation of hair follicle stem cells; 4, preparation of hair follicle stem cell regeneration tissue mud; and 5, filling concave scars. The method comprises the following steps: extracting hair follicle units to obtain skin regeneration tissues around hair follicle stem cells, culturing by using a special in-vitro culture solution, and further activating the hair follicle stem cells by using an anti-sensitivity instrument, so as to prepare tissue cell mud which can be used for filling; the purposes of filling and leveling concave scars, inhibiting scar hyperplasia, softening and smoothing the scars and repairing and beautifying the concave scars are achieved.

The invention relates to the field of protein, in particular to bioactive polypeptide LPYPYYA as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide LPYPYYA is Leu-Pro-Tyr-Pro-Tyr-Tyr-Ala. In vitro immune function regulation experiments and in vivo anti-aging experiments prove that the polypeptide LPYPYYA has better immune regulation functionand anti-aging activity; on one hand, the bioactive polypeptide LPYPYYA can enhance the in vitro multiplication capacity of lymphocytes and macrophages, improve the capability of the body for resisting external pathogen infection and reduce the disease incidence of the body; on the other hand, the activity of an in vivo antiperoxidase system can be improved; the function of the body for resistingthe exogenous irritation is enhanced, so that the body aging, senility and sickness probability is reduced; very important significance is realized on developing food, health products and medicines with the immune regulation function and the anti-aging function.

The invention relates to a method for facilitating in vitro multiplication of a bovine skeletal muscle satellite cell. The method comprises the following steps: obtaining a target sequence containing a bovine miR-133b gene sequence; building an expression vector pEGFP-C1-miR133b; transfecting the bovine skeletal muscle satellite cell by the pEGFP-C1-miR133b. By application of the method for facilitating in vitro multiplication of the bovine skeletal muscle satellite cell, the in vitro multiplication ability of the bovine skeletal muscle satellite cell is improved by obtaining over-expression miniature RNA miR-133b of the bovine skeletal muscle satellite cell and using the effect of the miR-133b, and differentiation is effectively inhibited. The over-expression vector of the miR-133b is built by using the pEGFP-C1, and amplification and differentiation of a bovine muscle satellite cell are regulated by the miniature RNA, so as to obtain a high-purity bovine muscle satellite cell. A good cell model is provided for researching the function and the effect of each regulatory factor in amplification and differentiation processes of the muscle satellite cell.

The present invention relates to the field of proteins, in particular to a bioactive peptide KQSLPPGLAVKDLK and its preparation method and application. The amino acid sequence of the bioactive peptide KQSLPPGLAVKDLK is Lys‑Gln‑Ser‑Leu‑Pro‑Pro‑Pro‑Gly‑Leu‑Ala‑Val‑Lys ‑Asp‑Leu‑Lys. The in vitro immune function regulation experiment proved that the biologically active peptide KQSLPPGLAVKDLK has a good immune regulation function. The biologically active peptide KQSLPPGLAVKDLK of the present invention has the ability to enhance the in vitro proliferation of macrophages and lymphocytes, improve the ability of the body to resist external pathogen infection, reduce the incidence of the body, improve the quality of life, and is useful for the development of food and health products with immune regulation functions. and drugs are of great importance.

The invention relates to the field of bioengineering medical materials and discloses a method for building tissue engineering limbus corneae. The method comprises the following steps of putting fresh pig corneoscleral limbus matrix containing a lamina elastica anterior into a decellularized limbus corneae protecting liquid added with a detergent and nuclease for oscillating acellular treatment; putting decellularized limbus corneae into the decellularized limbus corneae protecting liquid for rinsing to obtain the decellularized limbus corneae; inoculating seed cells into the decellularized limbus corneae for amplification in vitro; obtaining the tissue engineering limbus corneae when the seed cells are amplified to 3-5 layers on the decellularized limbus corneae, wherein the decellularized limbus corneae protecting liquid is a PBS buffer solution containing 5-12g / L hyaluronic acid, 5-20g / L chondroitin sulfate, 3-10g / L dextran and 2.5-5mg / L L-tobramycin. Human limbus corneae can be replaced with the tissue engineering limbus corneae material.

The invention relates to the technical field of biology, and discloses a DNA molecule and a preparation method and application thereof, and a recombinant virus and a preparation method and application thereof. The DNA molecule contains a 5'non-coding region sequence, a coding sequence of a capsid protein C into which a coding sequence of Nano luciferase is inserted, a coding sequence of a membrane and a membrane protein precursor prM, a coding sequence of an envelope protein E, a coding sequence of a non-structural protein and a 3 'non-coding region sequence which are connected in series, the 5'non-coding region sequence, the coding sequence of the capsid protein C, the coding sequence of the membrane and membrane protein precursor prM, the coding sequence of the envelope protein E, the coding sequence of the non-structural protein and the 3 'non-coding region sequence are derived from the yellow fever virus. The cDNA sequence corresponding to the genome RNA of the recombinant virus is the same as the sequence of the DNA molecule. The DNA molecule and the recombinant virus can be used for screening yellow fever virus treatment drugs.

The invention relates to the field of proteins, in particular to a biologically active peptide RLAFIAHPKLG and a preparation method and application thereof. The amino acid sequence of the biologically active peptide RAFIAHPKLG is Arg-Leu-Ala-Phe-Ile-Ala-His-Pro-Lys-Leu-Gly . Through in vitro immunomodulatory function experiments, it was found that the biologically active peptide RLAFIAHPKLG has a good immunomodulatory function. The bioactive peptide RLAFIAHPKLG of the present invention has the ability to enhance the in vitro proliferation ability of lymphocytes, can promote macrophages to secrete cytokines, improve the body's ability to resist infection by external pathogens, reduce the body morbidity rate, improve the quality of life, and has immunomodulatory effects on development Functional foods, health products and medicines are of great significance.

The invention relates to the field of protein, in particular to a bioactive peptide KQSLPPGLAVKDLK as well as a preparation method and application thereof, and the amino acid sequence of the bioactivepeptide KQSLPPGLAVKDLK is Lys-Gln-Ser-Leu-Pro-Pro-Gly-Leu-Ala-Val-Lys-Asp-Leu-Lys. In-vitro immune function regulation experiments prove that the bioactive peptide KQSLPPGLAVKDLK has a very good immune regulation function. The bioactive peptide KQSLPPGLAVKDLK disclosed by the invention has the effects of enhancing the in-vitro proliferation capability of macrophages and lymphocytes, improving thecapability of resisting external pathogen infection of an organism, reducing the morbidity of the organism and improving the quality of life, and has very important significance for developing foods,health care products and medicines with an immunoregulation function.

The present invention relates to the field of proteins, in particular to a bioactive peptide IAHPKLGKRIR and its preparation method and application. The amino acid sequence of the bioactive peptide IAHPKLGKRIR is Ile‑Ala‑His‑Pro‑Lys‑Leu‑Gly‑Lys‑Arg‑Ile‑Arg . In vitro immune function regulation experiments proved that the biologically active peptide IAHPKLGKRIR has a good immune regulation function. The biologically active peptide IAHPKLGKRIR of the present invention can promote the proliferation ability of lymphocytes in vitro, improve the immunity of the human body, and at the same time have a significant promotion effect on the ability of macrophages to phagocytize neutral red in vitro, improve the body's ability to resist external pathogen infection, reduce the body's It is of great significance to develop food, health care products and medicines with immune regulation function and improve the quality of life.

The invention relates to a skin renewal method. According to the skin renewal method, hair follicle stem cells are used as seed cells and silica gel dressing is used as a new cell transplantation carrier. The skin renewal method comprises the following steps: 1) cultivating and amplifying hair follicle stem cells; 2) cultivating dermal cells; 3) mixing the hair follicle stem cells cultivated and amplified in the step 1) and the dermal cells cultivated in the step 2); 4) dropwise adding mixed cells obtained in the step 3) into silica gel dressing for incubation; and 5) transplanting the cells and the silica gel dressing used in the step 4) to a part required to be subjected to skin renewal. The method provided by the invention not only can renew functional skin in the treatment of large-area skin injuries, but also can provide a simple validation method for the multipotency test on hair follicle stem cells in scientific research activities.

The invention relates to the field of protein, in particular to a bioactive peptide SEPKPIFF as well as a preparation method and application thereof, and the amino acid sequence of the bioactive peptide SEPKPIFF is Ser-Glu-Pro-Lys-Pro-Ile-Phe. In-vitro immunoregulation function verification experiments prove that the bioactive peptide SEPKPIFF has a very good immunoregulation function. The bioactive peptide SEPKPIFF can enhance the in-vitro proliferation capacity of lymphocytes, can effectively inhibit inflammation caused by oxidation of a body, improves the capacity of the body for resisting external pathogen infection, reduces the morbidity of the body, is greatly helpful to treatment of chronic diseases caused by inflammation, and has very important significance in developing food, health care products and medicines with an immunoregulation function.

The invention relates to a cell culture medium. The cell culture medium contains a basal culture medium, a serum substitute, linoleic acid, testosterone, vitamin A, L-ascorbic acid, polylysine, putrescine, and 2-mercaptoethanol. The cell culture medium of the invention can efficiently culture sebaceous gland cells in vitro, so that a cell proliferation rate, a survival rate after resuscitation, and an expression amount of lipid synthesis related proteins of different generations of the sebaceous gland cells are significantly increased, and thereby the assistance for extensively researching sebaceous gland physiological and endocrine functions, exploring pathogenesis of sebaceous gland related diseases, and developing drugs is provided.

The present invention relates to the field of proteins, in particular to a biologically active polypeptide FSPANKKLTPKKY and its preparation method and application. The amino acid sequence of the biologically active polypeptide FSPANKKLTPKKY is Phe-Ser-Pro-Ala-Asn-Lys-Lys-Leu-Thr-Pro-Lys ‑Lys‑Tyr. Through the in vitro immune function regulation experiment, it is verified that the polypeptide FSPANKKLTPKKY has a good immune regulation function. The biologically active peptide FSPANKKLTPKKY of the present invention can significantly enhance the in vitro proliferation ability of lymphocytes, and has the ability to increase the induction of macrophage nitric oxide under certain concentration conditions, improve the body's ability to resist external pathogen infection, and reduce the body's morbidity , improving the quality of life is of great significance to the development of food, health products and medicines with immune regulation function.

The invention discloses a mesenchymal stem cell culture medium without serum and xenogeneic components and application thereof. The present invention firstly discloses a mesenchymal stem cell culture medium, comprising a basal medium and an additive, the basal medium is a DMEM / F12 medium, and the additive is a human platelet lysate and / or human lipocalin 2. The invention further discloses the application of the mesenchymal stem cell culture medium in culturing mesenchymal stem cells. The mesenchymal stem cell medium provided by the present invention has no serum and no heterologous components, which ensures the safety of clinical application; the components are simple, the stability between batches is good, and the cell expansion ability in vitro is strong, which meets the requirements of industrial transformation; The immunoregulatory ability and multi-directional differentiation ability of mesenchymal stem cells are well maintained to ensure their effective function in clinical treatment.

The present invention relates to the field of proteins, in particular to a bioactive peptide SAPRHGSLGFLPRK and its preparation method and application. The amino acid sequence of the bioactive peptide SAPRHGSLGFLPRK is Ser‑Ala‑Pro‑Arg‑His‑Gly‑Ser‑Leu‑Gly‑Phe‑Leu ‑Pro‑Arg‑Lys. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide SAPRHGSLGFLPRK has a good immune regulation function. The biologically active peptide SAPRHGSLGFLPRK of the present invention can significantly promote the in vitro proliferation ability of macrophages, and at the same time increase the ability to induce nitric oxide, thereby improving the body's ability to resist external pathogen infection, reducing the body's morbidity rate, and improving life expectancy. Quality is of great significance to the development of food, health care products and medicines with immune regulation function.

The invention relates to application of m6A demethylase FTO in inhibition of NPM1 mutant leukemia cell proliferation, and belongs to the technical field of biomedical research. The technical problem to be solved by the invention is to provide a new molecular target for treating NPM1 mutant leukemia. The expression level of FTO in NPM1 mutant leukemia is higher than that of non-NPM1 mutant leukemia, and high expression of FTO is related to poor prognosis of a patient; the shRNA lentiviral vector or drug meclofenac sodium is utilized to target FTO, and the growth capacity of leukemia cells can be effectively inhibited. The m6A demethylase FTO is expected to become a novel molecular treatment target and a clinical prognosis index of the NPM1 mutant leukemia.