32results about How to "Enhance in vitro proliferative ability" patented technology

The invention relates to the field of protein, in particular to bioactive polypeptide LPYPYYA as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide LPYPYYA is Leu-Pro-Tyr-Pro-Tyr-Tyr-Ala. In vitro immune function regulation experiments and in vivo anti-aging experiments prove that the polypeptide LPYPYYA has better immune regulation functionand anti-aging activity; on one hand, the bioactive polypeptide LPYPYYA can enhance the in vitro multiplication capacity of lymphocytes and macrophages, improve the capability of the body for resisting external pathogen infection and reduce the disease incidence of the body; on the other hand, the activity of an in vivo antiperoxidase system can be improved; the function of the body for resistingthe exogenous irritation is enhanced, so that the body aging, senility and sickness probability is reduced; very important significance is realized on developing food, health products and medicines with the immune regulation function and the anti-aging function.

The invention relates to the field of bioengineering medical materials and discloses a method for building tissue engineering limbus corneae. The method comprises the following steps of putting fresh pig corneoscleral limbus matrix containing a lamina elastica anterior into a decellularized limbus corneae protecting liquid added with a detergent and nuclease for oscillating acellular treatment; putting decellularized limbus corneae into the decellularized limbus corneae protecting liquid for rinsing to obtain the decellularized limbus corneae; inoculating seed cells into the decellularized limbus corneae for amplification in vitro; obtaining the tissue engineering limbus corneae when the seed cells are amplified to 3-5 layers on the decellularized limbus corneae, wherein the decellularized limbus corneae protecting liquid is a PBS buffer solution containing 5-12g / L hyaluronic acid, 5-20g / L chondroitin sulfate, 3-10g / L dextran and 2.5-5mg / L L-tobramycin. Human limbus corneae can be replaced with the tissue engineering limbus corneae material.

Provided is a polypeptide, the amino acid sequence of which is SLPQ. The polypeptide is prepared by fermented skimmed milk by Lactobacillus helveticus. The polypeptide can enhance immunity of a body, so also provided are food, health products and medicines comprising the polypeptide.

The present invention relates to the field of proteins, in particular to a bioactive peptide GGYGSGGGSGGYGSRRF and its preparation method and application. The amino acid sequence of the bioactive peptide GGYGSGGGSGGYGSRRF is Gly-Gly-Tyr-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly ‑Tyr‑Gly‑Ser‑Arg‑Arg‑Phe. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide GGYGSGGGSGGYGSRRF has a good immune regulation function. The biologically active peptide GGYGSGGGSGGYGSRRF of the present invention can significantly enhance the in vitro proliferation ability of macrophages, can effectively inhibit inflammation caused by oxidation, has a good immune regulation effect, can improve the body's ability to resist external pathogen infection, and reduce the body's morbidity , improving the quality of life is of great significance to the development of food, health products and medicines with immune regulation function.

The present invention relates to the field of proteins, in particular to a bioactive peptide SAPRHGSLGFLPRK and its preparation method and application. The amino acid sequence of the bioactive peptide SAPRHGSLGFLPRK is Ser‑Ala‑Pro‑Arg‑His‑Gly‑Ser‑Leu‑Gly‑Phe‑Leu ‑Pro‑Arg‑Lys. After the verification experiment of immune regulation function in vitro, it was found that the biologically active peptide SAPRHGSLGFLPRK has a good immune regulation function. The biologically active peptide SAPRHGSLGFLPRK of the present invention can significantly promote the in vitro proliferation ability of macrophages, and at the same time increase the ability to induce nitric oxide, thereby improving the body's ability to resist external pathogen infection, reducing the body's morbidity rate, and improving life expectancy. Quality is of great significance to the development of food, health care products and medicines with immune regulation function.