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Bioactive polypeptide LPYPYYA as well as preparation method and application thereof

A technology for biologically active polypeptides and derivatives, applied in the protein field, can solve the problems of poor digestibility and absorption, no public performance, etc., and achieve the effects of reducing the incidence of the body, improving the vitality, and adjusting the body's immune activity and anti-aging activity.

Active Publication Date: 2018-04-13
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent discloses a macromolecular substance with poor digestibility and absorption. At the same time, the macromolecular biological activity disclosed in the patent has immune activity, and it does not disclose whether it has other properties

Method used

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  • Bioactive polypeptide LPYPYYA as well as preparation method and application thereof
  • Bioactive polypeptide LPYPYYA as well as preparation method and application thereof
  • Bioactive polypeptide LPYPYYA as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The artificial synthesis of embodiment 1 active peptide LPYPYYA

[0040] 1. Synthesis of Bioactive Peptides

[0041] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0042] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0043] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0044] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0045] 5. We...

Embodiment 2

[0081] Example 2 Bioactive Peptide Regulating Body Immunity Activity Experiment

[0082] 1. MTT method to determine the in vitro lymphocyte proliferation ability of biologically active polypeptide LPYPYYA

[0083] 1. Experimental materials and instruments:

[0084]Reagents and materials: experimental animals balb / c mice (male 6-8 weeks old, Animal Experiment Center, School of Agriculture and Biology, Shanghai Jiaotong University); milk-derived bioactive polypeptide LPYPYYA obtained in Example 1; mouse lymphocyte extract (purchased from Suo Laibao); RPMI1640 medium (purchased from GIBCO); 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT, purchased from from Amresco Company); Concanavalin (ConA, purchased from Sigma Company); bovine serum albumin (BSA, purchased from Genebase Company); pepsin (purchased from Sigma Company); pancreatin (Corolase PP, purchased from AB Company ).

[0085] Equipment: LRH-250F biochemical incubator, Shanghai Heng Technology Co., Ltd...

Embodiment 3

[0111] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0112] 1. The effect of biologically active polypeptide LPYPYYA on the structure of spleen tissue in vivo

[0113] 1. Experimental reagents and instruments:

[0114] Reagents: experimental animal ICR mice (male 5 weeks old), Shanghai Experimental Animal Center; D-gal, Sinopharm Chemical Reagent Co., Ltd.; paraformaldehyde, Sinopharm Chemical Reagent Co., Ltd.; sodium chloride, Sinopharm Chemical Reagent Co., Ltd. Ltd.; the milk-derived bioactive polypeptide LPYPYYA obtained in Example 1.

[0115] Instruments and equipment: CM-230 Mole ultra-clean water, Shanghai Moller Scientific Instrument Co., Ltd.; MilliporeMILLEX GP0.22μm filter membrane, Millipore Corporation; GL-22M high-speed refrigerated centrifuge, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.

[0116] 2. Experimental method:

[0117] (1) Modeling of animal aging models

[0118] After one week of adaptive feeding, the ICR mice were div...

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Abstract

The invention relates to the field of protein, in particular to bioactive polypeptide LPYPYYA as well as a preparation method and application thereof. The amino acid sequence of the bioactive polypeptide LPYPYYA is Leu-Pro-Tyr-Pro-Tyr-Tyr-Ala. In vitro immune function regulation experiments and in vivo anti-aging experiments prove that the polypeptide LPYPYYA has better immune regulation functionand anti-aging activity; on one hand, the bioactive polypeptide LPYPYYA can enhance the in vitro multiplication capacity of lymphocytes and macrophages, improve the capability of the body for resisting external pathogen infection and reduce the disease incidence of the body; on the other hand, the activity of an in vivo antiperoxidase system can be improved; the function of the body for resistingthe exogenous irritation is enhanced, so that the body aging, senility and sickness probability is reduced; very important significance is realized on developing food, health products and medicines with the immune regulation function and the anti-aging function.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide LPYPYYA and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soybean peptides, and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A23L33/18A61K38/17A61K8/64A61P39/06A61P37/02A61Q19/08
CPCA23L33/18A23V2002/00A61K8/64A61K38/00A61Q19/08C07K14/4732A23V2250/55A23V2200/302A23V2200/324
Inventor 张少辉张伯宇李云飞汪超林学海李阜烁范梦珠陈静
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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