156results about How to "Easy to survive" patented technology

The related treatment method for domestic garbage comprises: adding 0.5-1.0% aerobic microbe into the fermentation room; breaking, air separating, and collecting exudate for standby; taking first fermentation for 15-25day to let water content in compost material as 30-40%, and collecting exudate for standby; adding aerobic microbe again and former exudates to keep water content as 45-55%for second static fermentation for 25-35day. This invention has well effect with low cost.

The invention discloses a sprout cultivation method and device of machine-plugging rice seed, which comprises the following parts: sprout cultivation bed with frame structure, substrate, covering film and micro-hole permeating film. the sprout cultivation method comprises the following steps: 1. paving substrate; 2. paving soil on the sprout cultivation bed; strickling the surface; 3. irrigating to make bottom soil reach saturated condition; 4. weighing seed; sowing evenly; 5. covering sowing soil evenly; 6. covering film to conserve soil moisture; 7. managing seed bed; 8. cutting block to obtain the plant for plugging.

The invention relates to the technical field of soil improvement, and concretely discloses a saline-alkaline land soil conditioner, and a preparation method and an application thereof. Raw materials of the conditioner comprise 400-600 parts of a biomass decomposed material, 150-242 parts of a pyroligneous liquor, 200-300 parts of charcoal powder, 4-10 parts of urea and 2-10 parts of a microbial agent. All the components are synergistically combined, so the conditioner contains abundant organic matters, provides favorable conditions for crop growth, can improve the physical and chemical properties of the soil and slowly release nutrients, improves the fertilizer utilization rate and the water holding capacity of the soil, effectively reduces the pH value of the soil and the salt content ina soil solution, delays accumulation of salt in the surface soil, and has a good improvement effect on saline-alkaline land soil.

The invention discloses a siderophores-producing bacillus. The strain is paenibacillus illinoisensis preserved in China General Microbiological Culture Collection Center (CGMCC) on August 29, 2014, wherein the address of CGMCC is Courtyard No.3, No. 1 of Beichen West Road, Chaoyang District, Beijing, and the Preservation No. is CGMCC No. 9620. The strain of the siderophores-producing bacillus can produce siderophores in an iron-limited culture medium. Under the conditions of pots and fields, after a bacterium suspension of the siderophores-producing strain of the siderophores-producing bacillus is subjected to seed soaking treatment before peanut seeding and is subjected to root-irrigation treatment in the growth period; in calcareous soil, the strain can obviously improve the iron nutrition of peanuts in the growth period, and can increase the chlorophyll content of peanuts, enhance the root activity of peanuts, promote the accumulation of nitrogen, phosphorus and potassium in peanut plant bodies, increase the biomass of plants, improve the quality of peanuts and increase the yield of peanuts.

The invention discloses a method for breeding Australian freshwater lobsters. The method is characterized by comprising the following steps of building a lobster pond, and selecting a place which is great in air circulation and free of pollution sources, faces the sun and has convenient irrigation and drainage conditions; cleaning and disinfecting the pond, wherein quick lime and water are used for cleaning the lobster pond, and the consumption of the mixture is 100 kg per mu; conducting stocking of young lobsters, wherein the specification of the young lobsters is 2-4 cm, and the density of stocking is 6,000-8,000 per mu; putting baits, wherein the baits are put once in the morning and evening every day, the feeding amount accounts for 5-10% of the weight of the lobsters, and the feedingamount in the evening accounts for 70-80% of the total feeding amount of the whole day; managing the lobster pond, wherein checking and patrols need to be carried out frequently at the ordinary time,and aquatic plants on the bottom of the pond are supplemented in time; conducting disease prevention and control, wherein prevention and control are carried out mainly, and disinfection of the body surfaces of lobster bodies is the most important work during disease prevention and control in order to prevent pathogens from being brought into the pond; checking whether or not purse nets around thepond are firm enough at the regular time; conducting harvesting, wherein stocking and harvesting are conducted in the same year. Lobsters bred according to the method are large in size, fat, rich in nutrient and high in growth speed, yield and survival rate.

The invention relates to a skin renewal method. According to the skin renewal method, hair follicle stem cells are used as seed cells and silica gel dressing is used as a new cell transplantation carrier. The skin renewal method comprises the following steps: 1) cultivating and amplifying hair follicle stem cells; 2) cultivating dermal cells; 3) mixing the hair follicle stem cells cultivated and amplified in the step 1) and the dermal cells cultivated in the step 2); 4) dropwise adding mixed cells obtained in the step 3) into silica gel dressing for incubation; and 5) transplanting the cells and the silica gel dressing used in the step 4) to a part required to be subjected to skin renewal. The method provided by the invention not only can renew functional skin in the treatment of large-area skin injuries, but also can provide a simple validation method for the multipotency test on hair follicle stem cells in scientific research activities.

The invention discloses a yield-increase type culture method of polygonum capitatum. The method is that the reasonable seedling medium culturing and growth management are performed; a seedling bed is utilized for inoculating, rapidly propagating, cutting, reproducing and breeding; the stem sections of the seedlings in the seedling bed are utilized; one section with one bud and one leaf is inoculated from the seedling bed and then cut and planted into the soil; the specific field transplanting, field management and harvesting methods are adopted. The method has the characteristics of increasing the purity and quality of polygonum capitatum, high and stable in yield, and easy to be popularized.

The invention provides an organic compound microbial fertilizer and a preparation method thereof. The organic compound microbial fertilizer comprises the following materials according to mass percentage: 5%-10% of zymophyte liquid, 70%-80% of turfy soil, 14.5%-24.8% of rotten chicken manure and 0.2%-0.5% of diammonium hydrogen phosphate. The organic compound microbial fertilizer and the preparation method thereof have the advantages of being simple in operation process, short in production cycle, low in cost, complete in product function, high in quality, good in application effect, environmental-friendly and easy to popularize and providing a high-quality microbial organic fertilizer product for agricultural production.

The invention discloses a high-efficiency aerobic fermentation device of organic wastes. The high-efficiency aerobic fermentation device is characterized in that a storage bin is connected with material crushing and mixing equipment which is connected with a fermentation reaction bin; the fermentation reaction bin is internally provided with a stirring device, microbial strains are placed in the fermentation reaction bin which is connected with a blower; the fermentation reaction bin is internally provided with a temperature sensor and an oxygen concentration sensor; a finished-product output hole at the bottom part of the fermentation reaction bin is connected with a finished-product stack and the storage bin; a waste-gas exhausting hole at the top part of the fermentation reaction bin is connected with a waste-gas treating device; and a PLC controls the whole device. The high-efficiency aerobic fermentation device disclosed by the invention has the advantages that the occupied area is small, the fermentation period is short, the mechanization degree is high, tail gas has no secondary pollution, and a finished product meets the requirements on reduction, harmlessness, stabilization and recycling and reaches national standards in China.

The invention relates to the field of landfill, and discloses a combined landfill site closure system. The combined landfill site closure system comprises a landfill site body, wherein impermeable layers are arranged on the bottom part and the side surfaces of the landfill site body, drainage pipelines are arranged in the impermeable layers, and the drainage pipelines are connected with a sewage treatment station; an inner cavity of the landfill site body is filled with solid waste, a covering layer is arranged on the solid waste, and the covering layer is composed of a first crushed stone layer, a first geomembrane, a drainage net and a vegetation layer from bottom to top in sequence; and an exhaust pipeline is arranged in the first crushed stone layer, and an exhaust network is connectedwith a gas gathering station. Through the combined landfill site closure system, the problems that the impermeable performance of the landfill site closure system is poor, resources are wasted, and the landfill amount of the landfill site body is reduced due to the fact that a large amount of earth and stone is used in an existing landfill site closure system are solved, and the problem that leachate of the landfill site body pollutes groundwater is also solved.

The invention discloses an air layering seedling culture method of a red maple, and belongs to a seedling culture method of the ornamental red maple. The seedling culture method includes the following steps of peeling, mud ball packing, red maple plant nutrient and water management enhancing and field planting. A rooted branch which is directly separated from a red maple plant in an air layering mode is used as a seedling for planting, it is avoided that the seedling does not easily survive due to the fact the diameter of the seedling is too small, the large self-rooting seedling with the diameter being about 4.5 centimeters and the height being about 2 meters can be cultured within a short time, and the growth rate of the seedling and trunk forms are increased by effectively managing the seedling field planting process. The seedling can be rooted and bred on the branch to be clipped from the red maple plant, and therefore waste is utilized to culture the seedling, and seedling culture cost of the red maple is lowered. The steps of the air layering seedling culture method of the red maple are simple, the seedling of the red maple can be cultured on various occasions, and the application range is wide.

The invention discloses a mobile dun salix cheilophila planting method, which comprises the following steps that: sapling is fetched from salix cheilophila seed trees in spring, the sapling is soaked for more than 20 days and then is transported to a planting region, and the sapling is long stalked and deep planted on mobile dunes. By adopting the technical scheme provided by the invention, the cost is low, the forestation method is simple, the survival rate is high, and the formation speed is fast.

The invention relates to a new method for preparing bio-oil by treating duckweed biomass by using a hydrothermal method. The method is characterized by comprising the following steps of: (1) grinding dry duckweeds in a grinder to obtain fine duckweed particles; (2) weighing the treated duckweeds and putting the weighed duckweeds in a high-pressure reactor and adding water to react; and (3) extracting a mixture obtained through reaction by using organic solvents, separating and filtering extracts to obtain an organic phase, and carrying out rotary evaporation to remove the organic solvents, so as to obtain the bio-oil. The method for preparing the bio-oil with a duckweed biomass as a raw material has the beneficial effects that the raw material has high photosynthesis efficiency, strong adaptive capability to environment, short growth cycle, low energy consumption, little early-stage investment and low cost, is easy to cultivate in a large scale, does not occupy farmlands and relieves dependence on fossil fuel; besides, the high-moisture duckweeds do not need to be dried in a hydrothermal process, so that the energy consumption in early-stage treatment is low; and therefore, the method has good economic and social benefits.

The invention discloses a GPC3 (glypican-3) monoclonal antibody hybridoma strain 8G6, and a preparation method and application thereof, which belong to the technical field of cell engineering. The preservation code of the GPC3 monoclonal antibody hybridoma strain 8G6 is CGMCC (china general microbiological culture collection center) No.5427. The preparation method of the GPC3 monoclonal antibody hybridoma strain 8G6 includes: using GPC3 protein as immunogen to immunize a mouse; and fusing mouse splenocytes having serum titer more than 1:104 with SP2 / 0 myeloma cells, using an HATRPMI-1640 medium to screen fusion cells, and finally obtaining the hybridoma strain 8G6 by ELISA (enzyme-linked immunosorbent assay) and repeated limiting dilution. The GPC3 monoclonal antibody hybridoma strain 8G6 is high in yield of secretory antibodies, and is easy to survive, and the secreted monoclonal antibodies are high in titer, sensitive in reaction, easy to detect, low in production cost and widely applicable to detection of GPC3 protein expression.

The invention provides a coal mine domestic wastewater treatment device and a treatment method. The coal mine domestic wastewater treatment device comprises a grille well, an adjustment tank, a pre-sedimentation tank, and a simultaneous biological oxidation tank (SBOT), wherein the grille well, the adjustment tank, the pre-sedimentation tank, and the SBOT are orderly communicated along a coal mine domestic wastewater flow direction. The SBOT is separated into N little tanks along a direction perpendicular to the bottom of the SBOT. Each one of the N little tanks is provided with an upper flowchannel, carriers intercepted by an upper carrier interception grille and a lower carrier interception grille and a lower flow channel close to the bottom of the SBOT, wherein an aerated pipe is arranged between the lower carrier interception grille and the lower flow channel and a dredge pipe is arranged at the bottom of the SBOT and is communicated with the outside environment. The coal mine domestic wastewater treatment device and the treatment method can effectively reduce the content of organic matters in coal mine domestic wastewater, the content of ammonia nitrogen in coal mine domestic wastewater and the content of total nitrogen in coal mine domestic wastewater, avoid the use of a secondary sedimentation tank and an activated sludge backflow system, greatly simplify coal mine domestic wastewater treatment processes, and reduce coal mine domestic wastewater treatment device costs.

The invention discloses a remediation method for an acid heavy metal contaminated soil. The method mainly uses a botanical Jatropha curcas which has outstanding tolerance to extreme soil environments featured by high-concentration heavy metal residuals, being lacking of so many nutrient elements, having poor soil texture and the like; and in the method, seedlings are transferred in the contaminated place for field planting after starting seeds indoors so as to steadily remedy the contaminated soil. After being remedied by the Jatropha curcas, pH of the soil is improved, biological available heavy metal is greatly reduced, and harm for the environment is greatly relieved, so that the method has obvious ecological benefits. And simultaneously, production value per mu of bioenergy in each year is at least RMB 2850 yuan, so that the method has obvious economic benefits.

A closed live box on a fishing boat comprises a box body and is characterized in that the box body is arranged at the middle part of a deck of a boat body; a cabin cover is arranged at an opening in the upper end of the box body in an oppositely closed manner; a water inlet is formed in the side surface of the box body; a water drainage opening is formed in the bottom of the box body; the water inlet is connected with a water supply pipeline; a seawater pump is arranged on the water supply pipeline; the water drainage opening is connected with a water drainage pipeline; a sea bottom door is arranged at the bottom of the boat body; and the sea bottom door is in communication with the water supply pipeline and the water drainage pipeline. The closed live box on the fishing boat has the advantages that the structure is reasonably designed, the influence of the live box on stability is changed from an uncontrolled state into a controlled state because stability calculation is taken into consideration in the design stage, the influence of a free liquid surface and hidden dangers in the safety aspect are eliminated, and the economic benefits of the fisherman is also guaranteed.

The invention discloses a white chrysanthemum cultivating method in the technical field of cultivation of Chinese medicinal plants. The method comprises the following steps of weeding a seedling field; selecting chrysanthemum seedlings; breeding the seedlings to strengthen the seedlings, i.e., applying manure and plant ash into the seedling field according to the proportion that 100 to 300kg of the manure and 50 to 150 kg of the plant ash are applied to every mu of seedling field; trenching a planting field; digging holes, i.e., digging the holes on the middle line of a trench surface; transplanting, i.e., transplanting the chrysanthemum seedlings which are cultured through field planting into the holes, wherein two seedlings are planted in each hole; layering, i.e., layering for the first time: after the chrysanthemum seedlings grow to the height of 30 to 50cm, applying an organic fertilizer to the two sides of each row of chrysanthemum seedlings, wherein the dosage of the organic fertilizer is 1,000kg / mu, during layering, respectively separating branches to the two sides, enabling the branches to fall d to the ground, and putting soil onto the branches every 8 to 12cm; layering for the second time: when the length of the newly grown branches is 18 to 22cm, pushing down the branches in the direction where the branches are sparse, putting the soil onto the branches, and finishing layering for the second time before the end of July; pinching, and the like. The method is high in systematic degree, and fine in data and management program; the yield of the cultivated white chrysanthemum is high, so that the economic benefit is increased.

The invention discloses a pakchoi water culture method. The method comprises the following steps of seed treatment and germination accelerating, sowing, transplanting, management and harvesting. A water culture nutrient solution is prepared by adding 30-50 mL of tofu waste water, 10-20 mL of tea bran water, 20-30 mL of molasses fermentation liquor, 220-250 mg of ammonium nitrate, 5-8 mg of sodium molybdate, 3-5 mg of borax, 3-5 mg of calcium nitrate, 6-10 mg of ferrous sulfate, 1-3 mg of manganese sulfate, 1-3 mg of zinc sulfate and 1-3 mg of copper sulfate into each liter of pigsty liquid dung. The pH value of the nutrient solution is adjusted to be 5.8-6.5 by using citric acid. The electric conductivity is controlled to be 1.5-2.5 mS / cm, and the dissolved oxygen amount is 4-5 mg / L. Waste water resources are fully utilized, waste is turned into wealth, organic, safe and pollution-free pakchoi is harvested, and the popularization value is relatively high.

The invention belongs to the field of medical instruments, and discloses a cartilage defect repair material. The cartilage defect repair material is prepared from cartilage microparticles and II-typecollagen. The cartilage microparticles in the cartilage defect repair material are wide in source, which can be autogenous cartilage microparticles and allogenic cartilage microparticles, so that repeated operations can be avoided, and a wound in a patient is reduced. The cartilage defect repair material disclosed by the invention contains cartilage cells with repair capacity and active extracellular matrixes, and furthermore, a collagen material is high in biocompatibility, so that cells multiplied in the cartilage defect repair material or cells migrating from the body of a host are easy tosurvive and can be further multiplied. The cartilage defect repair material disclosed by the invention is required to be cultivated in vitro, and can be directly applied to repair of a cartilage defect.

The invention relates to the technical field of indoor insect breeding, in particular to an indoor mealybug and Aenasius bambawalei Hayat breeding method. The method has the advantages that water-cultured achyranthes bidentata is used to breed phenacoccus solenopsis tinsley and the Aenasius bambawalei Hayat, the developmental duration of the phenacoccus solenopsis tinsley and the Aenasius bambawalei Hayat can be shortened effectively, the survival rate and egg number per female of the phenacoccus solenopsis tinsley are increased, the parasitism rate and eclosion rate of the Aenasius bambawaleiHayat to the phenacoccus solenopsis tinsley are increased, and the method is especially applicable to the indoor breeding and researches of the mealybugs and the parasitic natural enemy of the mealybugs.

The invention provides water body ecology oxygenating equipment. The water body ecology oxygenating equipment is used in the cooperation of a physical lung-type oxygenating biological reef. The distance between the equipment and the reef is 1 to 2 meters. The equipment comprises at least one transparent hollow light guiding column, the surface of the light guiding column is covered by a layer made of a hydrophobic smooth material, the light guiding column is provided with a windmill or sweep blade, a floating plate, and a stirring device from top to bottom, the lower part of the light guiding column is also provided with an algae adhering part, and the bottom of the light guiding column can be rotated or shaken and is fixed on the water bottom. The provided equipment can stably provide oxygen to multiple water bodies to help the restoration and reconstruction of ecology in water, has the advantages of high efficiency, low cost, and simpleness in installation and maintenance, and can provide environment where organisms can survive easily.

The invention relates to a multi-enzyme system used for separating patient tumor tissue-originated primary cells. The system comprises components of, by weight: 0.02 to 0.2% of collagenase I-IV, 0.001 to 0.01% of hyaluronidase, 0.01 to 0.1% of trypsin, 0.02 to 0.2% of proteinase neutral, 0.001 to 0.01% of deoxyribonuclease I, and 5 to 50U / ml of elastase. The invention also relates to an application method of the system, an application of the system, and a kit formed by the system. The multi-enzyme system is advantaged in reasonable design. With the system, patient tumor tissue-originated primary cells can be separated with high specificity and high sensitivity. The separated primary cells are characterized by high survival rate, high purity, easy cryopreservation and easy resuscitation. The system and the kit can be used in medicine experimental evaluation, medicine toxicity testing, and medicine therapeutic performance determination. The system, the method and the kit are suitable to be widely popularized.

The invention discloses a floating type water quality improvement device, which comprises a purification unit shell, a hull, a drug pot and an oxygen-increasing pump. One end of the hull top is equipped with a motor box through support rods, and a storage battery is mounted at the bottom inside the motor box, a solar panel is mounted at the top of the motor box, and the solar panel is connected to the storage battery through a photo-voltaic controller, the drug pot is mounted on the hull below the motor box, ducts are installed on both sides of the drug pot bottom, and pass through the hull bottom inclinedly, and one ducts' ends far from the drug pot are both equipped with a penetration apparatus. The floating type water quality improvement device provided by the invention is equipped with the oxygen-increasing pump and stirring blades, the gas ejected from the oxygen-increasing pump is rapidly mixed with water under the stirring of the stirring blades, thus facilitating oxygen content increase of water, facilitating survival of aquatic species, and facilitating improvement of water quality.