The invention provides an MS-free
tissue culture method for bananas. Clump
shoot regeneration tissue culture liquid, subculture
tissue culture liquid and root taking
tissue culture liquid are adopted in the clump
shoot regeneration stage, the subculture stage and the root taking culture stage of bananas respectively, and the lump
shoot regeneration tissue culture liquid, the subculture tissue culture liquid and the root taking tissue culture liquid all contain a cassava
alcohol anaerobic
fermentation liquid culture medium. The technical scheme is adopted, cassava
alcohol anaerobic
fermentation liquid is used for replacing a basic culture medium MS,
normal growth in three different stages of banana tissue culture is not affected at all, production cost is greatly reduced, waste is turned into valuable materials, the method is more
environmentally friendly in the aspect of
ecological environment, cultured banana seedlings can achieve the effect of seedlings which are cultured through MS tissue culture in the prior art, the root taking amount is larger, roots are thicker and stronger, the period of
seedling establishment is earlier than that of the seedlings cultured through the basic MS culture medium,
transplantation and survival of tissue culture seedlings are easier, and favorable conditions are provided for large-scale tissue culture production.