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Bioactive peptide SAPRHGSLGFLPRK and preparation method and application thereof

A technology of biologically active peptides and derivatives, applied in the protein field, to achieve the effect of promoting in vitro proliferation ability, increasing the ability to promote, and improving the ability

Active Publication Date: 2021-05-11
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the functions of 60S ribosomal protein L3 protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide SAPRHGSLGFLPRK and preparation method and application thereof
  • Bioactive peptide SAPRHGSLGFLPRK and preparation method and application thereof
  • Bioactive peptide SAPRHGSLGFLPRK and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]The artificial synthesis of embodiment 1 active peptide SAPRHGSLGFLPRK

[0034] 1. Synthesis of Bioactive Peptides

[0035] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0036] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0037] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0038] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0039]...

Embodiment 2

[0075] The immune activity experiment of embodiment 2 bioactive peptides

[0076] 1. MTT method to determine the in vitro macrophage proliferation ability of the biologically active peptide SAPRHGSLGFLPRK

[0077] 1. Experimental reagents and instruments

[0078] Reagent: experimental animal balb / c mouse (male 6-8 weeks old) Animal Experiment Center, College of Agriculture and Biology, Shanghai Jiao Tong University; mouse spleen lymphocyte-derived bioactive peptide SAPRHGSLGFLPRK obtained in Example 1; 3-(4,5 -Dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) Amresco; LPS (lipopolysaccharide) Sigma; Bovine Serum Albumin (BSA) Genebase; Triple The solution is an aqueous solution containing 10% SDS, 5% isobutanol and 0.012mol / L HCl.

[0079] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus; Dragon WellscanMK3 m...

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Abstract

The invention relates to the field of proteins, and particularly relates to a bioactive peptide SAPRHGSLGFLPRK and a preparation method and application thereof. The amino acid sequence of the bioactive peptide SAPRHGSLGFLPRK is Ser-Ala-Pro-Arg-His-Gly-Ser-Leu-Gly-Phe-Leu-Pro-Arg-Lys. In-vitro immune regulation function verification experiments show that the bioactive peptide SAPRHGSLGFLPRK has a good immune regulation function. The bioactive peptide SAPRHGSLGFLPRK has a remarkable promotion effect on the in-vitro proliferation ability of macrophages, and meanwhile the ability of promoting the nitric oxide induction amount is improved, so that the ability of a body to resist external pathogen infection is improved, the morbidity of the body is reduced, the life quality is improved, and the bioactive peptide has very important significance in developing food, health care products and medicine with the immune regulation function.

Description

technical field [0001] The present invention relates to the protein field, in particular to a biologically active peptide SAPRHGSLGFLPRK and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] Immunomodulatory peptides generally refer to small peptides with relatively small molecular weights that have immunomodulatory activity. The currently disclosed immunomodulatory peptides are generally sm...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12A61K38/17A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61Q19/08A61P37/02A61P29/00A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55Y02A50/30
Inventor 张少辉占文静张伯宇
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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