37 results about "Platelet antibody" patented technology

The present invention relates to assay methods used for detecting the presence of PIF, and to PIF peptides identified using this assay. In particular, the present invention relates to flow cytomery assays for detecting PIF. It is based, at least in part, on the observation that flow cytometry using fluorescently labeled antilymphocyte and anti-platelet antibodies demonstrated an increase in rosette formation in the presence of PIF. It is further based on the observation that flow cytometry demonstrated that monoclonal antibody binding to CD2 decreased in the presence of PIF. The present invention further relates to PIF peptides which, when added to Jurkat cell cultures, have been observed to either (I) decrease binding of anti-CD2 antibody to Jurkat cells; (ii) increase expression of CD2 in Jurkat cells; or (iii) decrease Jurkat cell viability. In additional embodiments, the present invention provides for ELISA assays which detect PIF by determining the effect of a test sample on the binding of anti-CD2 antibody to a CD2 substrate.

The invention provides a method belonging to the field of cytometric bead array with high sensitivity for testing platelet specific antibody based on platelet basic experimental study. Platelet lysate is used to incubate the microsphere which is coated with platelet membrane glycoprotein monoclonal antibody, then goat-anti-human IgG polyclonal antibody labeled with phycoerythrin is added, which is analyzed in cytometric bead array. If autoantibodies exist on the surface of platelet, 'microsphere-platelet membrane glycoprotein monoclonal antibody-platelet membrane glycoprotein autoantibodies-goat-anti-human IgG polyclonal antibody labeled with phycoerythrin' complex structure is formed, the fluorescence intensity of the test microsphere is enhanced. The invention is easy to operate, and has mature technology, high sensitivity for testing platelet autoantibodies, which is good for basic experimental study of platelet antibody.

The invention relates to a novel platelet antibody kit using the micro-column gel technique and a preparing method thereof. The novel platelet antibody kit comprises a micro-column gel card, indicating erythrocytes and platelet antigens. The preparing method includes: preparing the micro-column gel card and preparing anti-IgG sensitization aldehydated erythrocytes. The novel platelet antibody kit has the advantages that sensitivity is high, specificity is good, quality is stable, the detection process is short, the preparing method is simple and easy, result are easy to judge, mass samples can be detected at the same time, automation is easy, and the platelet typing technique is made a routine clinical test item.

The invention provides a blood platelet antibody specificity identification method and a kit thereof. The method comprises the following steps: (1) connecting different blood platelet specificity glycoprotein to different fluorescent microsphere surfaces to obtain antigen specificity fluorescent microspheres for standby use; (2) adding the antigen specificity fluorescent microspheres into a sample to be detected to perform an incubation reaction, and washing for standby use; and (3) adding a fluorescent marked anti-human IgG into step (2) to perform the incubation reaction, washing, and performing flow cytometry detection. The blood platelet antibody specificity identification method solves the problems that the blood platelet antibody detection in the prior art mainly targets at an antigen-self blood platelet antibody complex on the surface of the blood platelet and does not detect free blood platelet antibodies in plasma or serum, and the detection result of the blood platelet antibody is imprecise; and moreover, the method is simple in steps, easy in operation, short in time comsuming and higher in efficiency.

The invention discloses an immunofluorescence chromatography test strip for detecting a platelet antibody and a detection method of the platelet antibody. The immunofluorescence chromatography test strip comprises a reaction film, a sample pad, an adsorption pad and a bottom plate, wherein the reaction film is placed on the bottom plate; a detection belt and a quality control belt are fixed onto the reaction film; the sample pad is overlapped with part of one side of the reaction film and placed on the reaction film and the bottom plate; the absorption pad is overlapped with part of the other side of the reaction film and placed on the reaction film and bottom plate; a sampling point is arranged on each of two sides of the sample pad; sample and fluorescence labeled secondary antibodies are dropped and washed; and after the reaction is done, the reaction result is indicated through fluorescein isothiocyanate labeled secondary antibodies. Due to the mode, according to the immunofluorescence chromatography test strip for detecting the platelet antibody and the detection method disclosed by the invention, the detection process is simple, efficient, accurate, low in cost and practical; semi-quantitative or quantitative detection can be realized; safe, efficient and scientific platelet transfusion in clinic can be guaranteed; and the precious platelet resources also can be saved.

The invention provides a platelet antibody detection kit and a use method thereof. The platelet antibody detection kit comprises a detection plate (1), six gel tubes (2) and an opening sealing film (3), wherein the six gel tubes (2) are arranged at the top end of the detection plate (1) in a left and right arranging way; the opening sealing film (3) is arranged above the detection plate (1), and covers the top ends of the six gel tubes (2); each of six gel tubes (2) contains an anti-human globulin reagent and sephadex; the first gel tube and the sixth gel tube are respectively a positive contrast tube and a negative contrast tube, and the second gel tube, the third gel tube and the fourth gel tube are reaction tubes. The platelet antibody detection kit has the advantages that anti-human IgG and IgG sensitized erythrocyte are used as indication systems to replace anti-human IgG-coated aldehydated erythrocyte, the detection result is clear and accurate, and the repeatability is strong; the effect that detection result can be accurately judged by the naked eye and automatic robot at the same time, and the detection result is easily standardized.

The invention relates to the technical field of machines, in particular to a method for artificially synthesizing platelets in vitro in a fluid movement mode. The method comprises a platelet generating device, a vacuum suction device and a platelet enriching device. According to a device and the method for artificially synthesizing platelets in vitro in a fluid movement mode, a turbulence device can be used to simulate a blood turbulence system in the body, induce platelet production, forcibly block quasi-platelet aggregation, and apply a kinetic energy that forces quasi-platelets to split thequasi-platelets self, so that clean platelets can be obtained, and platelet antibodies do not exist. The platelet yield produced by the method is huge, and there is no significant difference in the aspects of functions between mature platelets in the body and the produced platelets. The produced platelets can be directly applied to the clinic, and since the produced platelets is not screened by an in vivo immune system, the produced platelets are not obvious in antigenic property, and no platelet antibodies are generated.

The invention belongs to the technical field of platelet antibody detection, and particularly relates to anti-screening cells suitable for platelet antibody detection and a preparation method and application thereof. The anti-screening cells are prepared by the following method of: 1) mixing three persons of O-type platelets with screened and confirmed antigen spectrums in an isovolumetric manner, cleaning with platelet cleaning solution, centrifuging, discarding supernatant, and retaining platelet precipitates; 2) resuspending the platelet precipitates in the step 1) by using platelet loading liquid, adjusting the cell concentration to (1.0-2.0)*10<10> / ml, and oscillating; and 3) cleaning again with the platelet cleaning solution after oscillation, resuspending the precipitates by using platelet preserving solution after cleaning, and adjusting the cell concentration to (2.0-5.0)*10<7> cells / ml to obtain the anti-screening cells suitable for platelet antibody detection. The platelet anti-screening cells prepared by the method can be applied to platelet antibody detection, and is long in preservation time, convenient to use and good in antigen preservation effect.

The invention relates to a platelet antibody detection method based on a nano material and a platelet preservation method. In the invention, nano composite particles prepared by wrapping PLGA with platelet membrane protein are applied to platelet antibody detection for the first time, which proves that the platelet antibody can be accurately detected by taking human-derived serum containing an anti-platelet antibody as a detection object, and good sensitivity is possessed. In the invention, improvement of clinical diagnosis of platelet immune abnormal diseases is facilitated, matched plateletsare provided for patients, a clinical infusion effect of the platelets is improved, clinical blood transfusion quality is increased, and precious blood resources are saved for the society at the sametime.

An enzyme ¿C linked immune detection method of patient blood platelet and cross ¿C match includes obtaining multiple clone antibody from blood platelet film antigen composite immune abimal and using it to carry out encapsulation then carry out closing, washing plate, adding patient sample, washing plate, adding substrate for color developing and stopping reaction.

The present invention relates to methods for the detection of human platelet alloantigens (HPAs) on human platelets, methods for the detection of human antibodies against HPAs, and diagnostic test devices for carrying our said methods.

The invention belongs to blood platelet clinical test and research and relates to a cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia. The cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia is high in sensitivity and specificity. The method comprises encapsulating beads through anti-human platelet membrane glycoprotein monoclonal antibodies, incubating platelet lysate and the encapsulated beads, and then adding in phycoerythrin marked goat antihuman immune globulin polyclonal antibodies to perform flow cytometry. If autoantibodies exist on the surface of platelets, a bead-platelet membrane glycoprotein monoclonal antibody-platelet membrane glycoprotein autoantibody-phycoerythrin marked goat antihuman immune globulin polyclonal antibody composite structure can be formed and embodied as increase of fluorescence intensity of detecting beads. The cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia is convenient to operate, mature in technology, high in sensitivity of blood platelet autoantibody detection and conductive to fundamental experimental research on platelet antibodies.

The invention provides a method for preparing freeze-dried platelets. The method comprises the following steps: 1) enabling platelets to be in contact with a pretreatment solution and a stationary solution in sequence; 2) freeze-drying the platelets treated in the step 1) in the presence of a freeze-drying preservation solution, wherein the pretreatment solution comprises lysine and collagen, thestationary liquid comprises glutaraldehyde and ethanol, and the freeze-drying preserving fluid comprises serum albumin, PVP and mannitol. Also provided herein are lyophilized platelets prepared by themethod and uses of lyophilized platelets. The freeze-dried blood platelets provided by the invention can keep the activity of blood platelet antigens during long-term storage, and a solution is provided for application of anti-screening blood platelets and standardized application of a blood platelet antibody detection kit.

The invention discloses a fully automatic platelet antibody detecting device. The fully automatic platelet antibody detecting device comprises an outer shell body, a sample loading arm module, an outer ring rotating disc device, a centrifuge, an incubation module, a heat dissipation module, a sample carrier, a reagent carrier, a reading camera, a scanning head, a scanning head supporting plate anda driving device fixing seat. The driving device fixing seat is fixed to the bottom plate of the outer shell body, a driving device is installed on the driving device fixing seat, the outer ring rotating disc device is installed on the driving device, the outer ring rotating disc is in a hollow structure, the centrifuge is installed in the hollow structure of the outer ring rotating disc, the incubation module and the heat dissipation module are arranged in the centrifuge, the sample carrier and the reagent carrier are fixed in thecircumferential direction of the outer ring rotating disc. According to the fully automatic platelet antibody detecting device, full automation of processes such asreagent distribution, sample distribution, oscillating mixing, incubation, centrifugation, washingand interpretation can be achieved, labor intensity of experimenters is greatly reduced, and the inspecting efficiency is improved.

The invention relates to the technical field of blood transfusion inspection, in particular to a platelet antibody detection kit, application and a detection method, and the platelet antibody detection kit comprises a microwell plate containing U-shaped holes and an anti-human IgG coupled microsphere or particle preparation capable of distinguishing colors by naked eyes. Red blood cells are replaced by microspheres of which the colors can be distinguished by naked eyes, the preservation time can be greatly prolonged under the action of the compound stabilizer, other components in the detection product are synchronously purchased, stored and used, the platelet antibody detection kit becomes a complete kit, and the stable preservation period can reach more than one year; and great convenience is provided for the production and transportation processes of anti-platelet antibody detection products.

The invention discloses a water-soluble adhesive rapidly separating method for detecting surfactant molecules of s platelet membrane, comprising steps of: mixing a platelet to be detected and a fluorescently-labeled antibody, and adding same to a water-soluble adhesive chromatography medium to obtain a mixed liquid; then performing centrifugal layering on ttheo mixed liquid and discarding an upper layer; and after adding a buffer solution for resuspending, obtaining a result. By means of combining said water-soluble adhesive chromatography and low-speed centrifugation, the method provided by the invention may separate the antibodies that are not combined with the platelet and other soluble protein molecules in a reaction liquid from the platelet-antibody compounds, which avoids tedious experiment procedures of multiple washing required during the operation process of a conventional method, greatly simplifies operation steps, saves operation time, and improves detection efficiency.

The invention discloses an immune nanometer magnetic bead enzyme-linked immunosorbent assay method for detecting a blood platelet antibody, and the method is characterized by comprising the following steps: (1) mixing magnetized blood platelets with a to-be-detected sample and reacting; (2) separating and washing the magnetized blood platelets which react in the step (1) by using a magnetic force; (3) adding an enzyme-labeled human secondary antibody for reaction; (4) adding a substrate, developing and ending the reaction to obtain a detection result. Through the way, the immune nanometer magnetic bead enzyme-linked immunosorbent assay method for detecting the blood platelet antibody, disclosed by the invention, is simple and convenient in detection process, high in efficiency, accurate and reliable in result and relatively high in application value when being used for clinically diagnosing blood platelet related immune diseases and preventing invalid blood platelet injection.

The invention discloses an immunofluorescence chromatography test strip for detecting a platelet antibody and a detection method of the platelet antibody. The immunofluorescence chromatography test strip comprises a reaction film, a sample pad, an adsorption pad and a bottom plate, wherein the reaction film is placed on the bottom plate; a detection belt and a quality control belt are fixed onto the reaction film; the sample pad is overlapped with part of one side of the reaction film and placed on the reaction film and the bottom plate; the absorption pad is overlapped with part of the other side of the reaction film and placed on the reaction film and bottom plate; a sampling point is arranged on each of two sides of the sample pad; sample and fluorescence labeled secondary antibodies are dropped and washed; and after the reaction is done, the reaction result is indicated through fluorescein isothiocyanate labeled secondary antibodies. Due to the mode, according to the immunofluorescence chromatography test strip for detecting the platelet antibody and the detection method disclosed by the invention, the detection process is simple, efficient, accurate, low in cost and practical; semi-quantitative or quantitative detection can be realized; safe, efficient and scientific platelet transfusion in clinic can be guaranteed; and the precious platelet resources also can be saved.

The invention provides a method for detecting platelet antibody specificity by using flow cytometry. The method comprises the following steps: (1) preparing platelet antigen specific fluorescent microspheres; (2) preparing a detection antibody; (3) preparing a platelet reference substance; (4) detecting a reaction system; and (5) detecting a sample to be detected. The invention also aims at providing a detection kit for detecting the specificity of the platelet antibody by applying the flow cytometry. The kit comprises platelet antigen specific fluorescent microspheres obtained by coating thesurfaces of different fluorescent microspheres with different anti-platelet specific glycoprotein antibodies, a microsphere buffer solution, a platelet reference substance, a platelet lysate, a cleaning solution, calibration microspheres and a detection antibody. The invention discloses a platelet specific antibody detection method. In the prior art, the platelet antibody detection mainly aims atthe platelet antibody of the detected person; compared with the prior art, the method has the advantages that the problem of free platelet antibodies in blood plasma or blood serum is solved, the typeof the platelet specific antigen (HPA) of the antibody existing in a detected person can be further determined, and the method is small in sample use amount, more accurate in result, simple in step,easy to operate and higher in efficiency.

The present invention relates to an in-vitro diagnosis device for detecting and / or identifying antigen and / or antibody from a sample of biological fluid, particularly from a sample of blood or sample of blood components. The invention also relates to different uses of this device such as the detection and / or the identification of red blood cells antigens, platelet antigens, viralantigens, bacterial antigens, parasite antigens, the detection and / or the identification of anti-red blood cells antibodies, antiplatelet antibodies, antiviral antibodies, antibacterial antibodies and antiparasitic antibodies.

The invention provides a traditional Chinese medicine composition for inhibiting and removing aplatelet antibody and curing idiopathic thrombocytopenic purpura, wherein honeysuckle capable of clearing away heat and toxic materials and good at removing blood poisonis is taken as a monarch drug in a prescription, and is used to remove the platelet antibody in the blood. Radix rehmanniae, cortex moutan, red peony root and ligusticum wallichii are taken as an adjuvant drug, and are used to cool blood, clear heat, improve the internal environment of blood heat of a human body, and prevent and control the internal base for the generation of a blood platelet; lithospermum, madder, nodus nelumbinis rhizomatis charcoal and radix notoginseng powder are used to cool blood, remove blood stasis, stop bleeding and improve the signs and symptoms of bleeding; divaricate saposhnikovia root is used to dispel wind and clear heat, and is compatible with the honeysuckle to avoid relapse of the disease, caused by the reason that wind heat enters the interior. Liquorice is a conductant drug, relaxes tension, detoxifies, and coordinates the drug actions of the prescription. The various medicines in the prescription are combined, the prescription is simple but complete, is in great accuracy and hits the pathogenesis, and the various medicines play the roles of detoxifying, cooling blood, dispersing blood stasis, removing ecchymosis and nourishing spleen and kidney together, so that the bleeding of an ITP patient is reduced, and the various clinical symptoms are further alleviated.

The invention discloses a technical platform for establishing plate-type immunofluorescence reaction-PCR instrument detection, and provides a plate-type immunofluorescence kit for platelet antibody detection and a preparation method thereof. The kit comprises a 96-well plate coated with an antigen and an antibody and a fluorescein labeled antibody, and the detection instrument is a fluorescent quantitative PCR instrument. The method and the kit for detecting the platelet antibody by the plate-type immunofluorescence reaction-PCR instrument are high in sensitivity, good in specificity and repeatability and capable of covering various factors causing the platelet antibody, results among holes can be compared more visually and clearly through fluorescence numerical values, misreading and misreading are avoided, and missed diagnosis and misdiagnosis are reduced.

An immunoassay includes forming first mixtures by reacting a combination reagent with serum of a subject; simultaneously forming second mixtures by reacting randomly selected platelet samples with the serum wherein in each mixture there are immunity compounds formed by combining the platelet antigens with predetermined antibodies in the serum, and other platelet antigens and other antibodies in the serum not forming the immunity compounds; preparing an interception device including receptacles and a filter net; placing each mixture in one receptacle; washing the mixtures wherein the mixtures forming the immunity compounds are intercepted by the filter net with others passing through; adding a signal sensing reagent to each receptacle; reacting the signal sensing reagent with the intercepted mixtures forming the immunity compounds to form final products; and performing a signal sensing to determine whether the final products contain anti-platelet antibodies and determine compatibility of cross matching of respective platelet samples.

The invention relates to the mechanical technical field, and in particular, relates to a device for artificially synthesizing platelets in vitro in a fluid motion mode, wherein the device includes a platelet production device, a vacuum suction device and a platelet enrichment device. According to the device and method for artificially synthesizing the platelets in vitro in the fluid motion mode, ablood turbulence system in vivo can be simulated by a self turbulence device, the platelets are induced to produce, quasi platelet aggregation is forcibly prevented, and a kinetic energy to force quasi platelet self-splitting is applied, the clean platelets are obtained, and no platelet antibodies exist. The platelets produced by the method have huge yield and no significant difference from the function of mature platelets in vivo, and can be directly applied in clinic; because of no screening by the immune system in vivo, the produced platelets have no obvious antigenicity and no platelet antibodies are produced.

The invention relates to the technical field of machinery, and discloses a device for artificially synthesizing platelets in vitro in a fluid motion mode, which is characterized by comprising an industrial personal computer, a main control box, a reaction tank, a movable clamping plate, a base, a rocker mechanism, a stand column, a vibration main rod and the like, wherein the main control box is located at the upper end of the stand column, the vibration main rod is located at the lower end of the main control box and in front of the stand column; and the movable clamping plate is located in the middle of the stand column and connected with a long sliding groove in the middle of the stand column through a sliding strip. The device can simulate a blood turbulence system in a body, induce generation of platelets, forcibly prevent quasi-platelet aggregation and apply kinetic energy forcing the quasi-platelet to split to obtain clean platelets; wherein the platelets can be directly applied to clinical application, and the platelets are not screened by an in-vivo immune system, so that the platelets can be rapidly separated from the platelets; the generated platelets have no obvious antigenicity, and no platelet antibody is generated.

The invention discloses a cleaning solution for washing a blood platelet antibody detection kit microporous plate. The cleaning solution mainly comprises sodium dihydrogen phosphate, disodium hydrogenphosphate, Tween 20 and an appropriate amount of purified water and is characterized in that the pH of the cleaning solution is 6.5-7.5 at 25 DEG C, and each 200mL of purified water contains 0.2-0.8gof sodium dihydrogen phosphate, 1.0-2.5g of disodium hydrogen phosphate, 0.5-1.5mL of Tween 20 and 7-9g of sodium chloride; the cleaning solution is used for washing the microporous plate after beingdiluted by 25 times through the purified water. Compared with a conventional cleaning solution, the cleaning solution has the advantages that negative cell button lunar halo can be eliminated evidently, and the cleaning solution is simple to prepare and low in cost; the shelf life of the unopened cleaning solution at room temperature of 10-30 DEG C is four months; the shelf life of the opened cleaning solution is two months.

The invention relates to the field of mechanical technology, in particular to a method for artificially synthesizing platelets in vitro in a fluid motion mode, including a platelet production device, a vacuum suction device and a platelet enrichment device. The device and method for artificially synthesizing platelets in vitro using its own turbulence device can simulate the blood turbulence system in the body, induce the production of platelets, forcibly prevent the aggregation of quasi-platelets, and apply a kinetic energy that forces quasi-platelets to split themselves , to obtain clean platelets without the presence of platelet antibodies. The platelets produced by this method have a huge yield and have no significant difference in function from mature platelets in vivo. They can be directly used in clinical practice, and because they have not been screened by the immune system in vivo, the platelets produced have no obvious Antigenicity, does not produce platelet antibodies.

The invention discloses an internet-based full-automatic platelet antibody detection system and a use method thereof, and belongs to the technical field of medical instruments. A full-automatic platelet antibody detection system based on the Internet comprises a machine body, a control system is arranged in the machine body, a partition plate is arranged in the machine body and divides the machine body into an upper-layer space and a lower-layer space, a centrifugal machine is arranged in the lower-layer space, and a rotating shaft connected with the centrifugal machine is arranged in the upper-layer space. The rotating shaft is provided with a plurality of detection parts which are distributed circumferentially, each detection part comprises two hanging basket assemblies which are symmetrically arranged on the rotating shaft, and the rotating shaft is internally provided with a balance rod; the device can be suitable for inspection of different numbers of detection cards at the same time, balance of the symmetrical hanging basket assemblies on the two sides can be rapidly adjusted, operation is very convenient, the detection work efficiency is improved, practicability is high, the hanging baskets for containing the detection cards are always horizontally placed before and after use, and rapid taking and placing of the detection cards are facilitated.

The invention belongs to the technical field of platelet antibody detection, and in particular relates to an anti-sieve cell suitable for platelet antibody detection, its preparation method and application. The anti-screening cells were prepared by the following method: 1) 3 portions of O-type platelets that had been screened to confirm the antigen profile were mixed in equal volumes, washed with platelet cleaning solution, centrifuged, the supernatant was discarded, and the platelet precipitate was retained; 2) Resuspend the platelet pellet from step 1) in platelet loading solution and adjust the cell concentration to (1.0‑2.0)×10 10 cells / ml, shaking; 3) After shaking, use platelet cleaning solution to wash again. After cleaning, use platelet storage solution to resuspend the pellet, and adjust the cell concentration to (2.0‑5.0)×10 7 cells / ml to obtain anti-sieve cells suitable for platelet antibody detection. The platelet anti-sieve cells prepared by the method can be applied to platelet antibody detection, have a long storage time, are convenient to use, and have good antigen preservation effect.

An application of the Ge-enriched Jinzheng mushroom powder in preparing the medicine for treating lupus erythematosus with high curative effect is disclosed.