A kind of anti-sieve cell suitable for platelet antibody detection, its preparation method and application

Abstract

The invention belongs to the technical field of platelet antibody detection, and in particular relates to an anti-sieve cell suitable for platelet antibody detection, its preparation method and application. The anti-screening cells were prepared by the following method: 1) 3 portions of O-type platelets that had been screened to confirm the antigen profile were mixed in equal volumes, washed with platelet cleaning solution, centrifuged, the supernatant was discarded, and the platelet precipitate was retained; 2) Resuspend the platelet pellet from step 1) in platelet loading solution and adjust the cell concentration to (1.0‑2.0)×10 10 cells / ml, shaking; 3) After shaking, use platelet cleaning solution to wash again. After cleaning, use platelet storage solution to resuspend the pellet, and adjust the cell concentration to (2.0‑5.0)×10 7 cells / ml to obtain anti-sieve cells suitable for platelet antibody detection. The platelet anti-sieve cells prepared by the method can be applied to platelet antibody detection, have a long storage time, are convenient to use, and have good antigen preservation effect.

Description

technical field [0001] The invention belongs to the technical field of platelet antibody detection, and in particular relates to an anti-sieve cell suitable for platelet antibody detection, its preparation method and application. Background technique [0002] Platelet antibodies are produced by the body's immunity to platelet surface or related antigens, and the antigens that stimulate platelet antibody production can be self or allogeneic. There are two types of platelet antibodies, one is mainly antibodies against human histocompatibility antigen (HLA) class I antigens, namely platelet surface-associated antibodies (PA IgG), and the other is platelet-specific antibodies against GP antigens (PB IgG ). GP antigens and HLA-I antigens stimulate the body to produce an alloimmune reaction, resulting in exogenous platelet destruction that shortens the lifespan and changes the function of platelets, which is an important reason for the ineffectiveness of platelet transfusion. , ...

AI Technical Summary

Problems solved by technology

However, under normal circumstances, the storage period of platelets is only 5-7 days, and the detection of platelet antibodies depends on platelet antigens, which makes the detection of platelet antibodies more difficult.

At present, there are no commercialized anti-screening cells in my country, so it is difficult to carry out platelet antibody detection clinically. The foreign reagents used in a few blood stations are expensive, and the detection cost is high, which increases the burden on patients.

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