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Platelet antibody detection kit, application and detection method

A technology for antibody detection and platelets, applied in the field of blood transfusion testing, can solve problems such as safety risks, complex reaction system, and short validity period

Pending Publication Date: 2021-04-23
宝锐生物科技泰州有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a platelet antibody detection kit, which is used to replace the existing detection kits, so as to alleviate the technical problems of short validity period, complex reaction system and safety risks in the prior art

Method used

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  • Platelet antibody detection kit, application and detection method
  • Platelet antibody detection kit, application and detection method
  • Platelet antibody detection kit, application and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] This embodiment provides a platelet antibody detection kit comprising the following components:

[0115] A. The composition of low ionic strength salt solution (10mL×1) includes 1.8% (g / ml) glycine, 12.5mM potassium dihydrogen phosphate, 12.5mM disodium hydrogen phosphate, 0.002% (g / ml) bromocresol purple , pH is 6.5, conductivity is 4.2mS / cm.

[0116] B. Microplate with U-shaped wells (96 wells, 8 wells x 12 strips, detachable): All U-shaped wells are coated with platelet-specific monoclonal antibodies, and each well contains 250 ng of platelet-specific antibodies. Microplates are sealed in vacuum plastic bags with desiccant.

[0117] C. Positive quality control (1mL×1): On the basis of negative quality control, it contains platelet-specific antibodies.

[0118] D. Negative quality control (1mL×1): It does not contain platelet-specific antibodies and is the serum of healthy individuals with type AB.

[0119] E. Anti-human IgG-coupled microsphere or microparticle pre...

Embodiment 2～6

[0125] This embodiment provides a platelet antibody detection kit, the components are the same as in Example 1, the only difference is that the mass volume percentage (g / ml) of polystyrene red microspheres is 0.05%, 0.1%, 0.2%, 0.3% and 0.4%.

experiment example 1

[0127] In this experimental example, the platelet antibody detection kit provided in the above-mentioned Example 1 is compared with the existing commercially available MASPAT kit kit for platelet antibody detection. The specific methods include:

[0128] Both use the same platelet suspension to detect the same clinical sample, a total of 500 clinical blood samples were detected, and the positive coincidence rate of the platelet antibody detection kit provided in Example 1 was calculated with the test results of the commercially available MASPAT kit as a reference standard and negative coincidence rate.

[0129] The specific implementation steps are as follows (the steps of this kit and the MASPAT kit are the same without special instructions, only the different parts are explained, except that the platelet suspension and the sample are the same, and the components in the respective kits are used for operation).

[0130] (1) Preparation of platelet suspension: Platelet-rich pla...

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Abstract

The invention relates to the technical field of blood transfusion inspection, in particular to a platelet antibody detection kit, application and a detection method, and the platelet antibody detection kit comprises a microwell plate containing U-shaped holes and an anti-human IgG coupled microsphere or particle preparation capable of distinguishing colors by naked eyes. Red blood cells are replaced by microspheres of which the colors can be distinguished by naked eyes, the preservation time can be greatly prolonged under the action of the compound stabilizer, other components in the detection product are synchronously purchased, stored and used, the platelet antibody detection kit becomes a complete kit, and the stable preservation period can reach more than one year; and great convenience is provided for the production and transportation processes of anti-platelet antibody detection products.

Description

technical field [0001] The invention relates to the technical field of blood transfusion testing, in particular to a platelet antibody detection kit, application and detection method. Background technique [0002] Platelet transfusion can be used to prevent and treat bleeding symptoms in patients with decreased platelet count or function deficiency, and to restore and maintain the normal hemostasis and coagulation function of the human body. However, long-term platelet transfusion may lead to serious consequences of ineffective platelet transfusion, that is, two consecutive transfusions of sufficient platelets did not improve the patient's clinical bleeding symptoms, and the platelet count did not increase significantly. Platelet antigens through blood transfusion, pregnancy and bone marrow transplantation can stimulate the body to produce anti-platelet antibodies to sensitize and destroy platelets, causing thrombocytopenia, leading to serious consequences such as petechiae,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N33/531
CPCG01N33/6893G01N33/6854G01N33/577G01N33/54313G01N33/531G01N2800/222
Inventor 严俊师丽佳刘涛蒋友红卿小红
Owner 宝锐生物科技泰州有限公司
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