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Water-soluble adhesive rapidly separating method for detecting surfactant molecules of platelet membrane

A separation method and a technique for activating molecules, which is applied in the field of medical detection, can solve problems such as cumbersome and time-consuming operations, and achieve the effects of saving operation time, improving detection efficiency, and simplifying operation steps

Inactive Publication Date: 2017-06-13
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

The washing process is cumbersome and time-consuming, which is limited in clinical application

Method used

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  • Water-soluble adhesive rapidly separating method for detecting surfactant molecules of platelet membrane

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Embodiment 1

[0021] see figure 1 , providing a rapid aqueous gel separation method for the detection of activated molecules on the surface of platelet membranes, see below for details.

[0022] The reagents and instruments used in the aqueous gel rapid separation method include: fluorescently labeled anti-CD63, fluorescently labeled anti-GPⅡb / Ⅲa, fluorescently labeled anti-P-selectin, physiological saline, aqueous gel chromatography medium, water bath, centrifugation machine, flow cytometer.

[0023] The composition of the aqueous gel chromatography medium and its preparation method have been disclosed in the invention patent with the patent number ZL201310710675.0, and the patent has obtained the patent right. In this example, the aqueous gel chromatography medium is obtained by dissolving 5 g of polysucrose and 0.1 g of gelatin in 100 mL of water.

[0024] The steps of the aqueous gel rapid separation method for the detection of platelet membrane surface activation molecules are:

[0...

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Abstract

The invention discloses a water-soluble adhesive rapidly separating method for detecting surfactant molecules of s platelet membrane, comprising steps of: mixing a platelet to be detected and a fluorescently-labeled antibody, and adding same to a water-soluble adhesive chromatography medium to obtain a mixed liquid; then performing centrifugal layering on ttheo mixed liquid and discarding an upper layer; and after adding a buffer solution for resuspending, obtaining a result. By means of combining said water-soluble adhesive chromatography and low-speed centrifugation, the method provided by the invention may separate the antibodies that are not combined with the platelet and other soluble protein molecules in a reaction liquid from the platelet-antibody compounds, which avoids tedious experiment procedures of multiple washing required during the operation process of a conventional method, greatly simplifies operation steps, saves operation time, and improves detection efficiency.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a rapid separation method of water-based glue for detection of activated molecules on the surface of platelet membranes. Background technique [0002] With the development of society and economy, the incidence of cardiovascular disease continues to increase, and the mortality rate ranks first among various diseases. Therefore, the study of early warning and prevention techniques for cardiovascular diseases has become an important issue in current clinical medical research. [0003] Platelet activation plays a very important role in the occurrence and development of cardiovascular diseases. Platelets are in a resting state in normal blood circulation. When the vascular endothelium is damaged or under the action of certain physiological and pathological stimuli, platelets undergo activation reactions such as adhesion, deformation, aggregation and release. The main pa...

Claims

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Application Information

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IPC IPC(8): G01N1/34
CPCG01N1/34
Inventor 陈晔洲李勇王红梅段生宝丁少华蒙青林魏双施
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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