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Cleaning solution for washing blood platelet antibody detection kit microporous plate

An antibody detection and microplate technology, which is applied in detergent compounding agent, detergent composition, surface active detergent composition, etc., can solve the problems such as the inability to effectively reduce the background signal signal-to-noise ratio, etc. simple effect

Inactive Publication Date: 2019-06-18
江苏中济万泰生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that the washing liquid in the prior art cannot effectively reduce the signal-to-noise ratio of the background signal, and to provide a washing liquid for platelet antibody detection kit microporous plate washing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] A washing solution for platelet antibody detection kit microwell plate washing,

[0015] Further, every 200 mL of purified water contains 0.2-0.8 g of sodium dihydrogen phosphate, 1.0-2.5 g of disodium hydrogen phosphate, 0.5-1.5 mL of Tween 20 and 7-9 g of sodium chloride. 6.5~7.5.

[0016] Dilute 25 times with purified water before use for washing the microplate.

[0017] Using respectively, the concentrated washing liquid that is currently used, the concentrated washing liquid that was configured 4 months ago, and salt water are easy to carry out washing comparison experiments. The results show that the washing liquid of the present invention can obviously eliminate Negative cells buckle the halo phenomenon.

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Abstract

The invention discloses a cleaning solution for washing a blood platelet antibody detection kit microporous plate. The cleaning solution mainly comprises sodium dihydrogen phosphate, disodium hydrogenphosphate, Tween 20 and an appropriate amount of purified water and is characterized in that the pH of the cleaning solution is 6.5-7.5 at 25 DEG C, and each 200mL of purified water contains 0.2-0.8gof sodium dihydrogen phosphate, 1.0-2.5g of disodium hydrogen phosphate, 0.5-1.5mL of Tween 20 and 7-9g of sodium chloride; the cleaning solution is used for washing the microporous plate after beingdiluted by 25 times through the purified water. Compared with a conventional cleaning solution, the cleaning solution has the advantages that negative cell button lunar halo can be eliminated evidently, and the cleaning solution is simple to prepare and low in cost; the shelf life of the unopened cleaning solution at room temperature of 10-30 DEG C is four months; the shelf life of the opened cleaning solution is two months.

Description

technical field [0001] The invention relates to a washing solution for washing a platelet antibody detection kit for microporous plates. Background technique [0002] In the study, it was found that washing the microtiter plate with PBST, for many ELISA experiments, especially for the detection of antigens or antibodies in serum, the background is high, and the OD value of negative samples is even as high as 0.2-0.5 (the smaller the serum dilution factor, the higher the background ), it is difficult to distinguish from the low-concentration detection target in the sample to be tested, which reduces the sensitivity and accuracy of detection. [0003] Therefore, there is an urgent need for a washing solution that can reduce the signal-to-noise ratio of the background signal. Contents of the invention [0004] The technical problem to be solved by the present invention is to overcome the defect that the washing liquid in the prior art cannot effectively reduce the signal-to-...

Claims

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Application Information

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IPC IPC(8): C11D1/74C11D3/60C11D3/06C11D3/04
Inventor 林裕翔
Owner 江苏中济万泰生物医药有限公司
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