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Immunofluorescence chromatography test strip for detecting platelet antibody and detection method of platelet antibody

An immunofluorescence and chromatographic test strip technology, applied in the field of medical testing, can solve the problems of unguaranteed quality and supply of domestic reagents, expensive SPRCA reagents, and inability to perform quantitative detection, saving platelet resources, reducing bleeding and death. Cases, scientific effects of platelet transfusion

Active Publication Date: 2013-10-02
SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The price of SPRCA reagents from foreign companies is extremely expensive, and the quality and supply of domestic reagents are not guaranteed
At the same time, MAIPA and SPRCA can only do qualitative detection, but not quantitative detection.

Method used

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  • Immunofluorescence chromatography test strip for detecting platelet antibody and detection method of platelet antibody

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] see figure 1 , providing an immunofluorescence chromatography test strip for platelet antibody detection, comprising a reaction membrane 1, a sample pad 2, an absorption pad 3 and a base plate 4, the reaction membrane 1 is located on the base plate 4, and the reaction membrane 1 is A detection strip 5 and a quality control strip 6 are fixed, the sample pad 2 partially overlaps one side of the reaction membrane 1 and is positioned on the reaction membrane 1 and the bottom plate 4, and the absorption pad 3 is connected to the reaction membrane 1. The other side of the membrane 1 partially overlaps and is located on the reaction membrane 1 and the bottom plate 4, and the sample pad 2 includes a first sample application point 7 and a second sample application point 8, and the first sample application point 7 is set on the side away from the sample pad 2 overlapping with the reaction membrane 1 , and the second sample adding point 8 is set close to the side of the sample pad...

Embodiment 2

[0030] Provide a kind of method that the test strip described in embodiment one is used for platelet antibody screening:

[0031] (1) Preparation of test strips

[0032] 1) Preparation of reaction membrane

[0033] Select Millipore HF 180 film with a backing as the reaction film, and cut it into a size of 30×1.8cm for later use. Use 0.01M pH 7.2 phosphate buffer to adjust the concentration of human IgG to 0.5 mg / ml, add Tween-20 with a volume fraction of 1%, and spray it on the surface of the NC membrane as a quality control line using a film-drawing device. The amount of scratch film was 0.5 μl / cm. After mixing monoclonal antibodies specific for HLAⅠ, GPⅡb / Ⅲa, GPⅠa / Ⅱa, GPⅠb / Ⅸ, GPⅣ, adjust the concentration to 0.5mg / ml with 0.01M pH 7.2 phosphate buffer (PBS), add volume Tween-20 with a fraction of 1% is sprayed on the surface of the NC film as a detection line using a film-drawing device. The interval between each line is 5mm. Immediately after scribing the film, put it ...

Embodiment 3

[0054] A method for the specific identification of platelet antibodies by the test strip described in Example 1 is provided:

[0055] (1) Preparation of test strips

[0056] 1) Preparation of reaction membrane

[0057] Select Millipore HF 180 film with a backing as the reaction film, and cut it into a size of 30×1.8cm for later use. Use 0.01M pH 7.2 phosphate buffer to adjust the concentration of human IgG to 0.5 mg / ml, add Tween-20 with a volume fraction of 1%, and spray it on the surface of the NC membrane as a quality control line using a film-drawing device. The amount of scratch film was 0.5 μl / cm. Adjust the concentration of the monoclonal antibodies specific for HLAⅠ, GPⅡb / Ⅲa, GPⅠa / Ⅱa, GPⅠb / Ⅸ, GPⅣ with 0.01M PBS with pH 7.2 to 0.5 mg / ml, and add 1% Tween -20, spray it on the surface of the NC membrane as a detection line with a scratcher, and the interval between each line is 5mm, that is, monoclonal antibodies with different specificities are immobilized on the surf...

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Abstract

The invention discloses an immunofluorescence chromatography test strip for detecting a platelet antibody and a detection method of the platelet antibody. The immunofluorescence chromatography test strip comprises a reaction film, a sample pad, an adsorption pad and a bottom plate, wherein the reaction film is placed on the bottom plate; a detection belt and a quality control belt are fixed onto the reaction film; the sample pad is overlapped with part of one side of the reaction film and placed on the reaction film and the bottom plate; the absorption pad is overlapped with part of the other side of the reaction film and placed on the reaction film and bottom plate; a sampling point is arranged on each of two sides of the sample pad; sample and fluorescence labeled secondary antibodies are dropped and washed; and after the reaction is done, the reaction result is indicated through fluorescein isothiocyanate labeled secondary antibodies. Due to the mode, according to the immunofluorescence chromatography test strip for detecting the platelet antibody and the detection method disclosed by the invention, the detection process is simple, efficient, accurate, low in cost and practical; semi-quantitative or quantitative detection can be realized; safe, efficient and scientific platelet transfusion in clinic can be guaranteed; and the precious platelet resources also can be saved.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to an immunofluorescence chromatography test strip and a detection method for platelet antibody detection. Background technique [0002] There are complex blood group antigens on the surface of platelets, including ABO antigens, HLA-I antigens and HPA antigens (present on platelet membrane glycoproteins GPⅡb / Ⅲa, GPⅠa / Ⅱa, GPⅠb / IX), these antigens can stimulate the body to produce corresponding antibodies and cause related immune diseases. [0003] Many clinical diseases with thrombocytopenia and bleeding as the main symptoms are caused by platelet antibodies, and correcting the symptoms of thrombocytopenia is often a direct requirement for choosing a disease treatment plan. The most common clinical thrombocytopenia diseases include: platelet transfusion ineffectiveness, maternal blood type incompatibility thrombocytopenia, primary autoimmune thrombocytopenia, secondary thrombocytopeni...

Claims

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Application Information

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IPC IPC(8): G01N33/577
Inventor 段生宝李勇尹焕才王红梅丁少华田晶晶陈晔洲史素霞李冬
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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