Platelet antibody detection kit and use method thereof

Abstract

The invention provides a platelet antibody detection kit and a use method thereof. The platelet antibody detection kit comprises a detection plate (1), six gel tubes (2) and an opening sealing film (3), wherein the six gel tubes (2) are arranged at the top end of the detection plate (1) in a left and right arranging way; the opening sealing film (3) is arranged above the detection plate (1), and covers the top ends of the six gel tubes (2); each of six gel tubes (2) contains an anti-human globulin reagent and sephadex; the first gel tube and the sixth gel tube are respectively a positive contrast tube and a negative contrast tube, and the second gel tube, the third gel tube and the fourth gel tube are reaction tubes. The platelet antibody detection kit has the advantages that anti-human IgG and IgG sensitized erythrocyte are used as indication systems to replace anti-human IgG-coated aldehydated erythrocyte, the detection result is clear and accurate, and the repeatability is strong; the effect that detection result can be accurately judged by the naked eye and automatic robot at the same time, and the detection result is easily standardized.

Description

technical field [0001] The invention belongs to the technical field of blood transfusion testing, and relates to a platelet antibody detection kit and a use method thereof. Background technique [0002] With the significant increase of patients with tumors and blood system diseases and the rapid development of clinical transfusion medicine, the amount of platelet transfusion is increasing year by year. Platelet transfusion is an effective measure to increase platelet count, prevent bleeding, and protect patients' life safety. Its safe, scientific and effective transfusion has become an inevitable requirement for the development of clinical blood transfusion technology. However, at present, most of the platelet transfusions in my country still use random transfusion (that is, "blind transfusion"). Improper platelets are quickly destroyed and cleared by the body's immune system after transfusion, resulting in frequent occurrence of platelet ineffectiveness, which not only wast...

AI Technical Summary

Problems solved by technology

In order to overcome the practical problems of other clinical platelet antibody detection methods such as low sensitivity, poor specificity, complicated clinical operation, long detection time, difficulty in interpreting results, and lack of corresponding reagent quality control; the existing microcolumn gel method for platelet antibody detection Indicating erythrocytes generally use chemical reagents (such as glutaraldehyde) to formaldehyde the erythrocytes, and then coat anti-human IgG to indicate the reaction results. Erythrocytes destroy the antigen structure, which is easy to cause missed detection, so the use is limited, and it is difficult to meet the clinical requirements for rapid and safe blood matching

Images