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Efficient mesenchymal stem cell culture solution without serum component

A technology of mesenchymal stem cells and culture medium, applied in cell culture active agents, tissue culture, animal cells, etc., can solve the problems of mesenchymal stem cells losing stemness, accidental differentiation of cultured cells, etc., to ensure purity and stemness, ensure Feasibility, growth-promoting effect

Inactive Publication Date: 2019-03-01
沈阳中心血站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The addition of these growth factors may lead to unexpected differentiation of in vitro expanded cultured cells, making mesenchymal stem cells lose their stemness

Method used

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  • Efficient mesenchymal stem cell culture solution without serum component
  • Efficient mesenchymal stem cell culture solution without serum component
  • Efficient mesenchymal stem cell culture solution without serum component

Examples

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Embodiment Construction

[0025] Describe the present invention in detail below in conjunction with accompanying drawing and embodiment, the following description is only the preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the premise of the method of the present invention, can also make Several improvements and supplements should also be considered as the protection scope of the present invention.

[0026] Example.

[0027] 1. Preparation of mesenchymal stem cell culture medium.

[0028] 1. Preparation of human platelet lysate.

[0029] (1) Take 2×10 11 L -1 The machine-collected human platelets were added to a 250ml centrifuge bottle and centrifuged at 6000g for 20min at room temperature.

[0030] (2) Remove the supernatant, add normal saline to wash the platelets, centrifuge at 2000g for 10min at room temperature and suspend with 15ml of normal saline.

[0031] (3) Freeze overnight in a -20°C refrigerat...

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Abstract

The invention relates to the technical field of biology, in particular to a mesenchymal stem cell culture solution. The mesenchymal stem cell culture solution is prepared from the following components: human platelet lysate, mycillin (Pen Strep), long-acting glutamine, a chemotactic factor XCL1, a chemotactic factor CCL3, a heat shock protein (HSP70), a telomerase inhibitor IFN-alpha 2b and a basal culture medium DMEM. The components of the stem cell culture solution are wide in source, cultured mesenchymal stem cells are high in purity, quick in proliferation and good in dryness, and the mesenchymal stem cell culture solution is suitable for extracorporeal large-scale culture to conduct preclinical study and related clinical study.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium of mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (Mesenchymal stem cells) are a type of adult stem cells with multi-directional differentiation potential. obtained from various organizations. Mesenchymal stem cells are non-terminal differentiated cells, which have both the characteristics of mesenchymal cells and the characteristics of endothelial cells and epithelial cells; as a type of pluripotent stem cells, they can develop into bone, Cartilage, muscle and other directions of proliferation and differentiation. Current studies have shown that mesenchymal stem cells have certain therapeutic effects on systemic lupus erythematosus, Parkinson's disease, and spinal cord injury. [0003] In recent years, the research on mesenchymal stem cells has been increasing, and the research results are gradually moving towards clinical applicatio...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N2501/105C12N2501/115C12N2501/135C12N2501/15C12N2501/165C12N2501/21C12N2501/998
Inventor 李剑平李晓丰
Owner 沈阳中心血站
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