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A culture medium and technology of peridinium, which is applied in the field of marine microalgae cultivation, can solve the problems of algae cells being easily polluted, and achieve the effects of promoting photosynthesis, comprehensive nutrition, and increasing yield
Active Publication Date: 2017-05-10
QINGDAO UNIV OF SCI & TECH +1
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Problems solved by technology
The traditional method of expanding algae cell cultivation is an open-air cement pond cultivation method, and algae cells are easily polluted
Method used
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Embodiment 1
[0028]This embodiment provides a kind of proto-peridinium culture medium, and the formula of this proto-peridinium culture medium is as follows: NH 4 NO 3 , 30mg; CO(NH 2 ) 2 (urea), 40mg; KH 2 PO 4 , 5 mg; NaHCO 3 , 500mg; MnCl 2 4H 2 O 26g, FeC 6 h 5 o 7 ·5H 2 O (ferric citrate) 20g; VB12, 1×10 -4 mg; VB1, 100mg; KCl, 3mg; Na 2 EDTA 12.4g, chitosan oligosaccharide 1g (molecular weight 1500-2000Da), seawater 1000mL.
[0029] The preparation method of the original peridinium culture medium, comprises the steps:
[0030] 1) Sterilize the seawater for use;
[0031] 2) According to the order and dosage in the medium formula, add it into the sterilized seawater in turn, stir it fully for 5 minutes after adding it, then it can be used for inoculation.
Embodiment 2
[0033] The present embodiment provides a kind of culture method of protodinoflagellate, comprises the steps:
[0034] The first stage: Erlenmeyer flask cultivation; seawater is high-temperature sterilized seawater filtered through a 0.45μm fiber membrane, with a salinity of 30-35. After adding medium components, adjust the pH to 7.9±0.3; indoor cultivation, air-conditioned temperature control , 20±3℃; fluorescent tube lighting, light intensity 5000-7000lux, light-to-dark ratio 12h:12h;
[0035] Use a 3000-5000mL Erlenmeyer flask for culture, the working volume accounts for 3 / 5 of the volume of the flask, and inoculate the algae in the logarithmic growth phase; the inoculation density is 2×10 4 cell / mL-5×10 4 cell / mL; Shake the flask 6 times a day; algae cell density reaches 2×10 5 cell / mL-3×10 5 cell / mL, transferred to the next stage of culture;
[0036] The second stage: culture in mineral water buckets; use 18L mineral water buckets for cultivation, and use sodium hypoch...
Embodiment 3
[0041] Embodiment 3 comparative experiment
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Abstract
The invention discloses a protoperidinuim culture medium and method. The protoperidinuim culture medium is composed of, by weight, 20-100 mg of NH4NO3, 20-60 mg of CO(NH2)2, 1-10 mg of KH2PO4, 100-800 mg of NaHCO3, 15-50 g of MnCl2 4H2O, 10-30 mg of FeC6H5O7 5H2O, 1*10-5-1*10-3 mg of VB12, 50-200 mg of VB1, 0.5-5 mg of KCl, 10-15 mg of Na2EDTA, 0.2-5 g of chitosan oligosaccharide with a molecular weight of 1500-2000 Da, and 1000 mL of seawater. The protoperidinuim culture method comprises the steps of phase one, conical flask culture and phase two, mineral water barrel culture. The protoperidinuim culture medium and method provides the optimal nutritive salt formula for protoperidinuim culture, thereby improving the yield of protoperidinuim poison and reducing the culture cost; compared with traditional outdoor open cement ponds expanded culture manners, culturing protoperidinuim in mineral water barrels is lower in cost, less prone to pollution and easier to operate and can greatly reduce workload, increase the growth speed of the protoperidinuim and shorten the culture period.
Description
technical field [0001] The invention relates to a formula of a protodinoflagellate culture medium and a cultivation method using a mineral water bucket as a container. The invention belongs to the field of marine microalgae cultivation. Background technique [0002] Due to its strong toxicity and unpredictability, shellfish toxins pose a serious threat to consumers' health and safety and offshore ecological security. It has become an ecological disaster and a major marine environmental problem that the international community has paid more attention to in recent years. Azaspiracid (AZA) is the latest but most toxic new type of fat-soluble shellfish toxin discovered among the eight existing shellfish toxins. Because of its strong toxicity, high residue and slow metabolism, it has become a The European Union, the United States, Canada and other countries focus on the object, not only set the limit standard at 160 µg AZA-1 eq / kg (AZA1-3), the value is generally lower than othe...
Claims
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