Method for producing biodiesel raw materials from Botryococcus braunii
A technology of botrytis branii and biodiesel, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of low bio-oil content of microalgae, achieve rapid growth, improve quality, and increase production Effect
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Embodiment 1
[0056] (1) Inoculate the triangular shaker flask containing Botrytis vulgaris into a sterile photobioreactor, and cultivate it under light in the sterilized bacteria medium; the photobioreactor is a bubbling closed reactor, including CO 2 Gas storage bottle, gas mixing device and gas flow meter; seed medium and culture conditions are as follows: sea salt 24g / L, CH 3 COONa 0.3g / L, NaNO 3 200mg / L, NaH 2 PO 4 50mg / L, Na 2 EDTA10mg / L, FeSO 4 ·7H 2 O 2.5mg / L, Vitamin B1 0.006mg / L, Vitamin B20.00005mg / L, the light source is a fluorescent lamp, the light intensity is 8Klux, the light quality is white light, the initial pH is 6.0, the ventilation intensity is 0.2VVM, CO 2 The concentration is 0.1%, the culture temperature is 23° C., cultured for 8 days until the logarithmic phase, and the seeds are collected for the next stage of fermentation.
[0057] (2) Cell collection adopts centrifugal collection technology. Under the condition of rotating speed of 4000rmp, centrifuge at ...
Embodiment 2
[0063] The steps are the same as in Example 1, but the photoautotrophic culture conditions are: the light quality is blue light, the light intensity is 9Klux, the initial pH is 6.5, the ventilation intensity is 0.6VVM, CO 2 The concentration is 1.5%, and the culture temperature is 25°C. The oil content reaches 36%, and the CN value is 53.0.
Embodiment 3
[0065] The steps are the same as in Example 1, but the photoautotrophic culture conditions are: the light quality is white light, the light intensity is 10Klux, the initial pH is 7.5, the ventilation intensity is 1.0VVM, CO 2 The concentration is 2.0%, and the culture temperature is 24°C. The oil content reaches 42%, and the CN value is 55.0.
[0066] Beneficial effects of the present invention:
[0067] The present invention provides a method for producing high-quality biodiesel raw material with Botrytis braziliana, in which Botrytis braziliana vulgaris is used as the algae species, using relatively mild light and light quality, sufficient nutrition, suitable nitrogen-to-phosphorus ratio, and suitable Temperature and other conditions can promote the rapid growth of microalgae cells, the accumulation of oil and the stability and improvement of oil quality;
[0068] The present invention can not only improve the oil output of Botrytis branii, but also effectively improve the q...
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