Method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves
A technology for the single cell and algae cell of Synechosporium, which is applied in the field of preparation of single cell of Synechosporium, which can solve the problems of long doubling time, single and stable traits, slow growth rate, etc., so as to strengthen the selection of germplasm and speed up the breeding speed , the effect of doubling for a long time
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[0027] 1. Collect the algal cells in the stationary phase and incubate the algal cells with Tween 80
[0028] The algae cells of Synechobacterium enter the stable stage. The algae cells change from green to orange due to the accumulation of carotenoids. At the same time, they will also produce and enrich hydrocarbons and lipids during the metabolic process. Only a very small part of hydrocarbons and lipids will be released. Secreted into the water environment, the oil droplets can be continuously released from the algae by gently pressing the cover glass with tweezers under the microscope, staining and observing with Nile Red fluorescent dye, a large amount of hydrocarbons and lipids can be found According to the accumulation of hydrocarbon lipids, the color of dyeing varies from orange red to golden yellow.
[0029] Use a 500-mesh sieve to collect 100ml of algae liquid (to exclude the single cells released by itself during the growth process) and dissolve it in 10ml of Tween ...
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