Method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves

Abstract

The invention discloses a method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves, which comprises the following steps of: collecting botryococcus braunii cells in a stable stage, and incubating the botryococcus braunii cells by use of Tween80; breaking the incubated botryococcus braunii cells by use of the mechanical force of ultrasonic waves; separating to obtain a single free cell; detecting the activity of the single cell and the like. The method disclosed by the invention successfully obtains the botryococcus braunii single cells, the nutrient substances and illumination are uniformly absorbed in a culture process, the preparation time is short, and the yield of the botryococcus braunii single cells is high.

Description

technical field [0001] The invention relates to a method for preparing a single cell of Synchoplasma spp., in particular to a method for obtaining a single cell of Symphyllum spp. by using Tween 80 and ultrasonic waves. Background technique [0002] (1) The research status and research value of A. [0003] Since Maxwell et al. found in 1968 that grape alginate populations are full of oily substances composed of unsaturated hydrocarbons, and the composition and structure of hydrocarbons produced by grape algae are very similar to petroleum, microalgae researchers all over the world have paid attention to it. The conditions for its growth and hydrocarbon production have been extensively studied. Botryococcus braunii (also translated Botrytis) is a photoautotrophic single cell widely distributed in land, freshwater and brackish lake water in temperate, tropical and continental climate zones. The hydrocarbon content of green microalgae can be as high as 86% of the dry cell wei...

AI Technical Summary

Problems solved by technology

, in the process of making Synechophaera from laboratory research and cultivation to industrialization, there are still some problems: 1. At present, it is still impossible to realize its large-scale cultivation, and the slow growth rate limits the industrialization of hydrocarbon production by this algae a big obstacle

Cluster algae is a kind of algal body that grows in clusters. The algae body is composed of dozens to hundreds of cells, and the size is about 300-500 μm. Since the algae body grows in colonies, the algae body Mutual shading affects algal body absorption of nutrients and light, etc., algal body growth is slow, and the doubling time is long; 2. Cluster algae are directly separated from nature, and their properties are generally single and stable. In the process of industrialization of energy microalgae, It is difficult to make a breakthrough in the production of hydrocarbons and lipids; 3. The reproductive mode of Synechobacteria is mainly asexual reproduction, mainly by fission. For the selection and breeding of fine algal species, it is difficult to carry out sexual reproduction. Breeding of improved species; 4. The colony growth state of Sclerophyllum not only limits its growth rate, but also the genetic background of multiple cells is not uniform, so it is difficult to carry out mutation breeding by chemical or physical methods

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