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Method for extracting botryococcus braunii oil from wet botryococcus braunii fronds

A technology of botrytis wet algae and botrytis branii, applied in the field of extracting algae oil, can solve the problems of high energy consumption in the drying process, harsh operating process conditions, and restrictions on wide application, so as to save extraction cost and energy consumption, and the extraction process is easy , The effect of simple operation process

Inactive Publication Date: 2012-08-01
INST OF PROCESS ENG CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The algae oil product of Botrytis braziliana is mainly hydrocarbon algae oil, and its chemical essence is mainly ether lipid substances, which are mainly distributed on the outer cell wall and are easily soluble in various organic solvents. The algae oil does not need to be used in the production of biodiesel The transesterification reaction can save part of the production cost. However, the high extraction cost of the existing hydrocarbon algae oil still limits the use of this microalgae for biodiesel production.
Organic solvent has good dissolubility to this ether lipid class substance, adopts organic solvent to extract mostly now, and the extraction rate of this method is affected by microalgae water content bigger (J.Frenz, C.Largeau, E.Casadevall.Biotechnol and Bioeng, 1989, 34, 755), because the organic solvent is difficult to contact with the hydrocarbon algae oil due to the hindrance of the extracellular hydration layer of the wet algae, the extraction rate is very low when the wet algae hydrocarbon algae oil is extracted. The extraction rate of extraction with n-hexane is generally only about 20%. Therefore, in order to overcome the obstacle of the hydration layer of the wet algae during organic solvent extraction, the wet algae are usually dried to remove the extracellular hydration of the wet algae. layer to obtain dry algae, and then make the dry algae body and non-polar organic solvent fully contact, thereby improving the extraction rate, if supplemented by shock, stirring, ultrasonic and other treatment methods, the extraction rate can reach up to 100%, but dry The process consumes a lot of energy and takes a long time, which increases the cost of hydrocarbon algae oil extraction, prolongs the production cycle, and affects the popularization of this method.
There are also studies on the extraction of hydrocarbon algae oil using supercritical fluid technology (Rui L.Mendes, Beatriz P.Nobre, Miguel T.Cardoso, Ana P.Pereira, Antonio F.Palavra.Inorganica Chimica Acta, 2003, 356, 328 ), but subject to its harsh operating conditions and high equipment investment constraints, the wide application of this method is limited
How to solve the problem that the existing non-polar organic solvents are blocked by the hydration layer on the surface of wet algae cells and cannot contact with hydrocarbon algae oil during the extraction of wet algae hydrocarbon algae oil is the key to improving the extraction rate of wet algae organic solvent extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, rapid and efficient extraction of hydrocarbon algae oil in the wet algae body of Botrytis branii

[0025] Brown botrytis is cultivated in the laboratory or outdoors on a large scale, and the biomass reaches 2g dry weight / L culture solution. A certain volume of microalgae culture solution is collected by filtration, flocculation, sedimentation, centrifugation and other methods to collect wet algae, and the wet algae Put it into a reaction container, add 4mL (1 times the volume of wet algae) ethylene glycol dimethyl ether reagent to the container at room temperature, shake it at 50rpm for 10min, then add 20mL of n-hexane (1 times the volume of wet algae) 5 times) Stir at 300rpm for 20min, collect the extraction solvent containing algae oil, distill under reduced pressure to recover the extractant, and obtain algae oil, extract once, the total extraction rate of algae oil is 84.9%.

Embodiment 2

[0026] Embodiment 2, Rapid and efficient extraction of hydrocarbon algae oil in the wet algae bodies of Botrytis branii

[0027] Brown botrytis is cultivated in the laboratory or outdoors on a large scale, and the biomass reaches 2g dry weight / L culture solution. A certain volume of microalgae culture solution is collected by filtration, flocculation, sedimentation, centrifugation and other methods to collect wet algae, and the wet algae Put it into a reaction container, add 8mL (2 times the volume of wet algae) ethylene glycol dimethyl ether reagent to the container at room temperature, shake it at 80rpm for 5min, then add 10mL of n-hexane (2 times the volume of wet algae) 2.5 times) stirring at 200rpm for 10min, collecting the extraction solvent containing algae oil, distillation under reduced pressure to recover the extractant, and obtaining algae oil, extracting once, the total extraction rate of algae oil was 87.6%.

Embodiment 3

[0028] Example 3, Rapid and efficient extraction of hydrocarbon algae oil in the wet algae body of Botrytis branii

[0029] Brown botrytis is cultivated in the laboratory or outdoors on a large scale, and the biomass reaches 2g dry weight / L culture solution. A certain volume of microalgae culture solution is collected by filtration, flocculation, sedimentation, centrifugation and other methods to collect wet algae, and the wet algae Put it into a reaction container, add 16mL (4 times the volume of wet algae) ethylene glycol dimethyl ether reagent to the container at room temperature, shake it at 100rpm for 8min, then add 5mL of n-hexane (4 times the volume of wet algae) 1.25 times) stirring at 100rpm for 15min, collecting the extraction solvent containing algae oil, distillation under reduced pressure to recover the extractant, and obtaining algae oil, extracting once, the total extraction rate of algae oil was 89.1%.

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Abstract

The invention relates to a method for extracting botryococcus braunii oil from wet botryococcus braunii fronds. The method for extracting botryococcus braunii oil from wet botryococcus braunii fronds comprises carrying out an extraction process on wet botryococcus braunii fronds by glycol dimethyl ether. The method comprises the following steps of 1, carrying out filtration, flocculation, settlement and centrifugation of a botryococcus braunii culture solution to obtain wet botryococcus braunii fronds, 2, putting the wet botryococcus braunii fronds obtained by the step 1 into an extraction container, adding an ether reagent into the extraction container, and carrying out shaking treatment, 3, adding a C5-C10 alkane solvent into the extraction container, carrying out extraction with stirring, and collecting the botryococcus braunii oil-containing extraction solvent, and 4, carrying out reduced-pressure distillation of the botryococcus braunii oil-containing extraction solvent obtained by the step 3 to obtain the botryococcus braunii oil. The method provided by the invention has the advantage that wet botryococcus braunii fronds as raw materials are directly used in hydrocarbon extraction of botryococcus braunii oil so that the problem that wet microalgae has a low extraction ratio and long extraction time is solved.

Description

technical field [0001] The invention relates to the fields of bioengineering technology and chemical energy, in particular, the invention relates to a method for extracting algae oil from wet algae body of Botrytis branici. Background technique [0002] Since the first industrial revolution in the 18th century, the world has been consuming all kinds of fossil energy (such as coal, oil, natural gas, etc.) at an alarming rate, but its reserves on the earth are limited, leading to many energy crises affecting the world economy. Development, the extensive use of fossil energy has caused human beings to face two major crises: energy shortage and environmental degradation. Therefore, the development of renewable new energy and carbon dioxide emission reduction technologies has become an important task in the 21st century. As a new type of renewable energy, the development and utilization of biomass energy can not only help to eliminate the constraints of global economic developmen...

Claims

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Application Information

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IPC IPC(8): C10G1/04
Inventor 刘春朝郑森徐玲王锋郭晨
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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