Method for inducing Botryococcus braunii B12 to efficiently accumulate linolenic acid

Abstract

The invention provides a method for inducing Botryococcus braunii B12 to efficiently accumulate linolenic acid. The method is characterized by adopting the following steps : 1) preparation of algae solution : under the conditions of the temperature being 23+ / -2 DEG C, the light intensity being 1900+ / -1001 x and the ratio of light to dark being 12h / 12h, a 1 / 4 BG11 culture medium is adopted for culturing Botryococcus braunii B12 algal strain cells from 28 days to an exponential phase, an algae solution used for plant growth regulator induction is obtained, and BG11 nutritive salt with twice concentration is added once every 14 days during the culture period; 2) accumulation of linolenic acid: 200ml of the algae solution in the exponential phase is taken and placed into a 300ml conical flask, then a plant growth regulator ETH is added, the concentration of the plant growth regulator ETH in the algae solution is 0.01+ / -0.001 mg / L, induction treatment is performed at 23+ / -2 DEG C for 14+ / -1 days, manual shaking of the algae solution is not less than three times every day in the treatment stage, time intervals between adjacent shaking of the algae solution are not less than 2 hours, and the accumulation of linolenic acid in the algae solution is realized. The method is simple and feasible, the cost is low, and the accumulation of the linolenic acid content in the Botryococcus braunii B12 algal strain cells can be remarkably improved in a short term.

Description

technical field [0001] The invention provides a method for efficiently accumulating linolenic acid by inducing the B12 strain of Staphylococcus branici, belonging to the field of biotechnology. Background technique [0002] Botryococcus braunii belongs to Chlorophyta, Chlorophyceae, Chlorococcales, Botryococcusaceae, and Botryococcus. Freshwater unicellular microalgae. Brown Botrytis contains higher exopolysaccharides, fatty acids, especially hydrocarbons. In culture, its hydrocarbon production is 0.3% to 76.0% of the dry weight of biomass, usually 25% to 40%, and up to 86% in natural samples, which is much higher than the hydrocarbon content of other microorganisms (almost all less than 1%), the average calorific value of its hydrocarbon production is relatively high, and 1kg of grape hydrocarbon can be completely burned to release 3.0×10 4 ~4.2×10 4 kJ heat, atmospheric CO after combustion 2 There is no net increase in content and the composition and structure of the ...

AI Technical Summary

Problems solved by technology

Although the above-mentioned relevant patented technologies related to the purification and preparation of linolenic acid are advanced, none of them involves the content of growth regulators inducing Botrytis brachyphylla to accumulate linolenic acid, and the technical process is relatively complicated and expensive, which undoubtedly increases the production cost. and the difficulty of technology promotion