Method for preparing microalgae biodiesel
A technology of microalgae and biodiesel, which is applied in the fields of biofuel, petroleum industry, and fat production. It can solve the problems of low biodiesel production, low catalytic efficiency, and long reaction time, and achieve strong environmental adaptability and improved catalytic efficiency. , the effect of large reaction interface
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Embodiment 1
[0026] Preparation of Botrytis branii culture medium: Glucose 25mg / L, NaNO 3 1.8g / L, KH 2 PO 4 1.0mg / L, chitosan 1.2mg / L, biogas slurry 8mg / L, f2 vitamin 4mL and seawater 1L;
[0027] A method for preparing microalgae biodiesel, comprising the following steps:
[0028] S1. Cultivation of Botrytis braziliana: use Botrytis brawny as raw material, inoculate it into the culture medium at an inoculation density of 4 million cells / mL, and irradiate it with red and blue LED supplementary light for 10h / day, and the red light in the LED supplementary light The ratio with blue light is 1:1, the light intensity is controlled at 4500LX, the culture temperature is controlled at 25°C, cultured for 7 days, the density of Botrytis brachyphylla reaches 60 million cells / mL, and the Botrytis brachyphylla cells are collected;
[0029] S2. Grease extraction: After concentrating and drying the staphylococcal algae cells collected in step S1, use ultrasonic waves to break the wall of staphylococ...
Embodiment 2
[0034] Preparation of Botrytis branii culture medium: Glucose 20mg / L, NaNO 3 2.2g / L, KH 2 PO 4 1.1mg / L, chitosan 1.0mg / L, biogas slurry 7mg / L, f2 vitamin 2mL and seawater 1L;
[0035] A method for preparing microalgae biodiesel, comprising the following steps:
[0036]S1. Cultivation of Botrytis braziliana: use Botrytis brawny as raw material, inoculate it into the culture medium at an inoculation density of 3 million cells / mL, and irradiate it with red and blue LED supplementary light for 12h / day, and the red light in the LED supplementary light The ratio with blue light is 2:1, the light intensity is controlled at 4200LX, the culture temperature is controlled at 24°C, cultured for 7 days, the density of Botrytis brachyphylla reaches 60 million cells / mL, and the cells of Botrytis brachyphylla are collected;
[0037] S2. Grease extraction: After concentrating and drying the staphylococcal algae cells collected in step S1, use ultrasonic waves to break the wall of staphyloc...
Embodiment 3
[0042] Preparation of Botrytis branii culture medium: Glucose 30mg / L, NaNO 3 2.4g / L, KH 2 PO 4 1.3mg / L, chitosan 0.8mg / L, biogas slurry 9mg / L, f2 vitamin 3mL and seawater 1L;
[0043] A method for preparing microalgae biodiesel, comprising the following steps:
[0044] S1. Cultivation of Botrytis braziliana: use Botrytis brawny as raw material, inoculate it into the culture medium at an inoculation density of 5 million cells / mL, and irradiate it with red and blue LED supplementary light for 14h / day, and the red light in the LED supplementary light The ratio with blue light is 3:1, the light intensity is controlled at 4000LX, the culture temperature is controlled at 23°C, cultured for 8 days, the density of Staphylococcus branici reaches 60 million cells / mL, and the cells of Staphylococcus branici are collected;
[0045] S2. Grease extraction: After concentrating and drying the staphylococcal algae cells collected in step S1, use ultrasonic waves to break the wall of staphy...
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