Culture method for improving yield of botryococcus polysaccharides

Abstract

The invention discloses a culture method for improving the yield of botryococcus polysaccharides, comprising the following steps: culturing purified botryococcus to a logarithmic growth period, selecting botryococcus liquid in logarithmic growth, performing induced mutation under the irradiation of argon ion laser, then inoculating to a BG11 liquid medium, and culturing under the conditions that the temperature is 28-33 DEG C, the illumination intensity is 43-86mumol.m<-2>.s<-1> and the illumination period is 14 hours every day; performing ultrasonic radiation treatment after culturing 2-3 days, continuously culturing the botryococcus subjected to the ultrasonic radiation treatment for 12-13 days under the conditions that the culture temperature is 28-33 DEG C, the illumination intensity is 43-86mumol.m<-2>.s<-1> and the illumination period is 14 hours every day, and performing centrifugal separation and sterile water washing on the obtained culture solution to obtain botryococcus rich in polysaccharides. By combining the argon ion laser induced mutation with the ultrasonic treatment, the yield of the cultured botryococcus is high, many polysaccharides are accumulated, and the polysaccharide content of the botryococcus can reach 35.99% of the dry weight.

Description

(1) Technical field [0001] The invention relates to a method for cultivating grape algae with antibacterial effect and high polysaccharide production. (2) Background technology [0002] Environmental safety, aquaculture safety and other issues brought about by the over-exploitation of the fishery and the rapid development of the aquaculture industry have become more and more prominent, and the long-term abuse of drugs has resulted in increased bacterial resistance, drug residues, and environmental pollution. Outstanding issue. At the same time, with the development of industrialized high-density farming, a large amount of artificial feed is used, which dissolves a large amount of bait residues and excrement of aquatic animals in the water, causing serious pollution of the breeding water and causing serious damage to the growth and development of the farmed animals. At present, the substitutes of feed antibiotics on the market are mainly probiotics or traditional herbal medi...

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Problems solved by technology

[0004] On the other hand, most of the widely used antibiotics come from terrestrial actinomycetes and fungi, and long-term development has depleted terrestrial microbial resources.

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